Entamoeba histolytica (causing amoebiasis)
cma Optom te
e. leone ng
othe envionment teres
at ‘rere
(Ctopreste Pret wt)
videos P.O BB
De ay
rate ticity estat ser
cone ‘atu route nective .
48 ProtozoologyMebane
‘Cronin nrg membrane
ian ‘aematophagous
ayesone
Parcels erntton rom Estamos cl antes! commana
Untaind prepartons
E naea Trephazate Ecol
cower Ofepesn Corpus ean
Car toeite Puowopeds Baal
sate ovement Stugpish
Pape Nepal
i Ns recta gas wh
ewraly Ring ct gor
Aedbloodcats RBCs) ——_esions ‘cues cy. veoh )
coe, baci no RBCS
“18-80 15-50 ym
Precystand nie ost
emt Ofosam = arlar
yb ake ins la cng
Rosaretasie cyanide! incon sre tac
tes Sromdaltars
Glycogen masses: Giycogen masses:
pet
fae ‘Supe Ran
ane Wat Corps tain
ous otis
‘treacle nel ih re ‘react a wih
cenit tensome coe hanonores
m Ratetcromialten oars shoe
0-20 ft trary 1-35
Intestinal protozoa 49Entamoeba histolytica (causing amoebiasis) (continued)
Morphology (continued)
Ioaineprparatons
E netytca Wri Ect
rn, stise ‘syoopen Brown, compet
Srsyoanie yew Oflam Cconsiocus ganrty
Yoon ingwn cena Nal Nace marian wih
‘cen anesome
eto of arose) anos ty
‘Stains by ron homatonyin
“=
ap
Pky pon Se nr
2 ee
zane
a
el
ss mer
‘Sonal black central dt Karyosome ec acct @ &
ee ae ea
me how comin plautt
| wa
| ee Ta
ee oe
oat
aoa |
_
ie ee
a sonra
:
Se ee es
Compal rset See toot
‘ecules maybe
a
50 ProtozoologyPathology
Invasion of the large intestine
=
seen
goer
eo tcretine
Eee
“Tho primary ——_rvasoneh mcsavia cys
weet apr ay
oneness wi esa
“eee pac ir necescassng poston
ae
Fleseshane? Lag beind-esenson
Extrsionin Musclris rucesee lave eit
mucosa ‘ecumuaton of aresbae supertalk
(Gtr exons of crac
Formation Atscrses may cones iri
cfebuses Tusa
(eres ay dou wth eset
enon
Deep sare mucosae every perc
fxonsion —_{areclyrva esse)
‘age moss eub-minoan, venme
sduoaonea
Petts
Fenore
Surordng areca wacton ane
Rblatepollratin
‘amass under cedenaaus mucosa with
“real scar reco ave ad anosbas
Siroundeg gandonaous ese ora min oops,
‘a tries,
Bart aca fea tase
xtritostinal lesions 23052
Intestinal protozoa 51Entamoeba histolytica (causing amoebiasis) coninaa)
‘Eazsntestnal sions in amoebiasis
eens nt Fate hens et Fath hen
i se
i i a
f Ma om =
Ot on
Patra
(Serco
Sean en
frm at
bari
oc sures ses
SSoming tee eo
+ regular margins + Amoabae digest pathways ints tissue terte
Nee? andthe
‘ie sy +E rng ein ree onon
Direc extension Faemaiogenous pread
‘52 ProtozoologyLaboratory diagnosis
Diagnosis depends primarily on demonstration of haematophagous trophozoites of E. histolytica in stool samples, aspirates
from intestinal and other organs, biopsy material (pinch biopsy at proctoscopy or sigmoidoscopy and surgical biopsy from
clsewhere) and in mucus from rectal ulcers. LISA are available for the detection of Einfamoebu antigen and specific E. histolytica
lectin antigen in faecal samples. Serology is the method of choice for diagnosis of amoebic liver disease.
Facal appearances in ameblcdyeniany
‘Character
Nakad ye Faoalatior ‘Almas poset
Moos Noten
Notaburcat
Microscopie 1 Baca Numerous
2 Pie cale Scan, wel present
5. Re hod cl tenn ova
| Lge macrophages Notateatre
5 Charcot Lydencysas May be preset bt are rorspacte
6. Howmatohagisropbznes of htaics reser
‘Cure Usual ined retro rosa abort lath ELISA used fo spe enseton
FE ois fom ot bephazole. Enee eechophores tom cared tophaente
SSanatoratve.
Haamatphagous amosberophootes
(@) ist mirescopy (Tiere san (HAE sain
Notes
‘Vegetative E. histolytica when seen is actively motile and moves purposefully, There are finger-like, clear pseudopodia and
Ingested red cells. No nucleus can be seen. Precysts or cysts found in semi-formed or solid stool have typical nuclear characteristics
(1-4 nuclei) and glycogen and chromiial bars can be demonstrated
Diagnostic tests
Polymorph leucocytosis, Examination of stool samples may show cysts and trophozoites of E. histolytica. Serological tests (FAT,
ELISA, cellulose acetate precipitin, latex agglutination) but serology is postive in no more than 75% of cases of amoebic colitis
Examine aspirated material for F histolytica. Histology of rectal and colon biopsy material
Intestinal protozoa 53Other intestinal amoebae
Life cycle
So. ten
(seen fe at 7
"ron envionment inten |
Eneystation
(exept noi
Peete
‘yats vgaave fares fDi)
TE enrereon normed sno,
epee oe und arose
Morphology
Unstained
‘Vegetative forms (vephozotee)
Evfanoebe ci Enlinex nano ltarosta——Detamcobo——Entanoate —Enumoabeclipor—_Enanocbs
‘nso ‘aa Snover ms
‘Sue um SSB TSE
Moat ‘Soa Feyacive ——Veyscive ave
| te lite tre ovr ‘Aundart ‘Aountere
Peewdopod | Bunt naiy Suni mariy ‘But. cesr_—Leatitecear—Fngve, car Faget, ear Frere, doar
wus oe .% E
‘Ahave arr ecto cyt Ingest BCH Noingne RCE Moingned RBC
Endopiasm cc Sreearans fo ia rosea ings Ingest
Nucleus igo Gene Gen Two,cnleion Garey
| tacos = ‘tao ino roe
Preys
runt up, dacarge ed pares bata ec)Entamoeba coi Endoimax nana Iedamoaba——Dietamaeba “Entamoeba Entamoeba dispar Entamoeba
atch ‘aoyea ‘naman
‘Ofeplean ‘With hematoxyin, stars Bushey
ae Stain black xcept gycogen as clear area
‘Nusoor Ai
sion | PO @ g
Merérane Tee Thin Tick Deleate
Crromatin on Coase None Soneliras Nore Fin granuies
‘mererane ‘andar
otra! Central gran ‘Smal ool
Kiem | Oominrgeety Lae Lane! rane
‘Fan natwork | Maybe comin Nochromain —Nociromain Decale fis. No on seen
ets
Fariass Haass
Pathogenicity | Harioss Hrmioss armies Dspued Invasive commensal commensal
| comes arta conmer
Entamoebs col ests odamooba butschi cysts Entaroebs hstoytealdspar cysts
Intestinal protozoa 55