Elechophoresis (A2) In electro phoveacs there i$ no mobile phoae, ancl Only Gama, hit herdral molecules, besve thomghy The buffs sludion oboirg the plate . The origineh sput will pave bain Sepscetad too Horse spats. eo 4 t-2 setriaee moe Par shtamic eed oyna yane reaults of stechophera sts of plyetse, gistemic oxi ond lysine of pH=7 9° ti cH—C—o- tni—cH—C— Ht—cH—c—o" CH cH r n = rE [ rn Ne Iysine struchores of glycine , glutamce accel omel Lysine ot pila 7Get Elechophovasis , Paper ofectophersuis even thansh con ba usscl cto Sepsrote minphere of fone but the metho ct is jmprrersa . Get cleat phoraats 43 Commonly usec iin industrial, analutical or yassorch thulohmtals, fer paper wets ter paper wicks sample oan cathase ufer gel electophoresis apparatus. Tha moxn Compohmts vord ih a get obeecrophereacs aholysis ores + an phtetophorssis chamber. +o gel support meclinin soaked ih conchcctg buff +O Meana of _ on electric firlol- 29, a powerpack, + probs for detackilg and for mesainiig the sapere ral bro lacey * me srtracting the inde vidhack products ,Gut chet phortss covers a range of trehei puss thet is uaa to Séprrate, analyse anol purify michorss f biological Imoleenks such as proterns ondd mackie ely. Thase techarpuss con be ocloptad to : TPrecours tha rellstive bmoates of Macro mola cules + prepare. brackere acre ancl pLypsptides for Sepusheitg tha compohudt Mnehomtrs » 29. purhe onol pyrimidine baste amd altho acccls . *Saperate. proteus , 80 antibodies con ba ravsad , Redon Gel Elactophorssds + The Sasple mixture is placed ih the Sample well. The tmivoture is Sepscotnel into itt constitusnds Appigng con best’ Pik ts the gal ; which is socked in a Leguid buffer , The gh as a sponge —Lihe stuchire bagel or a theres - dimensional polymere artwork, It haa the trrcture of a folly - Gok oe word becauar their plopertics con be Preccsnlg comdrollsce duming thech preparcdioh , Om al hay are move Artes Cobley stuble a0 a Support median «The componsets ih the Sample bitters hove an elecbiced charge becouse probing, Like amino oes, Carrg either om ouerall positive on ovrrall ragative chorgs , deponcling on the pl of thesr enuiton mast. Nucleic aeide such aa decry riborrclece accck CWA) are Uauslly negodively charged (at the phosphate Groupe in the chasn) at the pH vorcl Loy thes Siprradion uachy slecthapho vases The Woleeuntes move th rhepons to an ghedr-cal Frobet applicd acrons the bixtuns . The vote of prograss of tha Molecules depends on thar Sine, chorge and shepe. On Stparetion, thee Componsnds are concentrated into hawde or zoken , Each band ow Bore. Com be quowhifredt vathg a Variety of musthocts Users smsitive tebwignss, sch 0a bioassays on silva steehang 1 Aharnts a0 Swath as (oY Com be defected,Applicetioe of Gel Elechopharasca . Checking the adulteration ot Poo ela + chromosome Sapuanecieg “DNA Finger priwhing : chawraefarising the chemicals reaporarchle frm aller gn'c Symptome + screen jnfonts’ melk for a-lacte globulin , protrin whith is lethal for small babire, Sceprroions baascl on Rete of Wa'qnacio, The vate of migredion of toch componset Ih the, mixture aenoss the grb becomes constomt when the focea of attrach'on batiienn the wluchoole ono ths, sppositely charged comporsnt 14 spac to tha bichon faver of the madiuts ragishing the wechion for thot port'culer spaccae . The relative votes of which compohsds mous ancl Hthuncfore the exhiet to which play typsrate are inPlerreak by the shrangth of the Aske, the nehere ofthe gel amol the fuarewdbiing buffer: Ha velocity of o molecule is chvactly prpectional to the voltage gradient ators the gel. 2. Size: Smaller molacukes io grate qaichen than larger molecules Comping te sane charga . So Shape ? & molecule with lote of side ~chaina expenieneey more fictohal ruistance than a Lineon molecule of the same mass ancl chonge onc will therefore move bore. slouty . & Buffer pH : proteche exist 2 gutttervons Like. mine acids, omel Cam bs ether positively or hag atively chargrl becouse they coutacn hoth acidic anol howe groups. The axtrt and devection of Porisation depincls on the pl of the buffer . S. Terapecatune 1 & HSE Im trmprraturs Com upssd op elacho phoresis , but com alee davctovethe proteita, Tha temprrotoya iS horirally controllecd by cooling the plate with a Plow of water vnrdarnacdh . Tivo ts og whicdrephror-ees If a numdosr of components ih salple have sihe'lon electrophoretic mobilitize , a complte seprrotion moy nat be achraved . Tints cose , oct the buffer medium ob the gol Support Con be alfmnsd . nothin option is to conduct Botany alatropherii in dleffer st buffers that con often Stpor ake components + ‘Exsro'se | a. White down the formula for the Fuvin ih which pers volite weulel marily ecict ih agucons solution . CHa c-H He-c-H rs HINT Soy H b. How wold thas change if A Small amunnd OF Aychrogen Fone were actolict ? C. What factors determing the vate of movemet of melecdes daring abubephoress ? a. CHs ue ae «1 40 eG - OG. 6. The COOM Grau becomes protonotect. Gis CH; pl Meso | eHt > e~e-H HN-C=c% ° sor Non Afi-C~ 0% q cee ee cH H C. Elachsphorers — rate of movement of mo lecnls determinad by 1 Change , size ; shape omel tempered .Exsrecse 2 A mixture of these Bhi oes iS Seperctad by gol elect phovssds The three cimino acrels Ore glycine, Valine ancl phenyl alasits . HsN-CH-CoOH H,N-CH-Cool — H2N-CH-Coolt i 4 Hel, chs glyene Gly ) Cus q ali ne (Val valine (Val) alenita (he) a. The ebecthophovsain i6 Caried oh ina buffer solushion of pH=l0 Drom Ha fons prasont ih theta alkokina condshions , br Draw a chetch of the electrogram yor wonkel topect (vieweet Brom abuve), labelling the aimiie acitle 00 Gly, Vat anc Phe. CH) Exploty Yorn onstuse fo pot bli), Worksn, at FH =(0, preset of OH” In hgh concentradtion oH +H t—> Heo E ee N-CH— oo” HW -CH-COOT Han CH 00" neds baa aly) manny be forthset, onotthe 7 largut CPhAy) ©. amino curd «Gly Val Phe mowing tine we ae Tease ie r = stort vot Phe Gi) Each of the ton will have &-I chorga ot pll=00, so the size, oF the Pens 15 the only fector Involved Ih thee seprachon . The molecales ars supinted according to sree, with ¢he gmmallatSome medical apphicefions of gek theetropharesds Neural tube malformations com be ditedtect fom protec leaking From the nuevos system of a Portus jato Fhe auariod'c Plucd Cth Plait enveloping the forts). Analysing ther protetne con tndcofe spiral probleme in new —borh babies + i des Our Koo petypephele, spots show up ih o wo — dimansionol abuctropherogram of hutnon swsot Hawg of thane hove been pravonaly Umidestified . Alcohet obise . The avfout of atcohot abuse Com be inveah'gatect by ina blood. This }s because. excess aloohed is assoccated with changsa im aocdic proterna om ol abrcoprateine 1h blood plasma. “Bish eye! dese nse + Ths is an inherited condition ih which Lipid 43 laid down in the eysa making them appecr opogue Like Fish's 2yey, Hs madicak maine 's dyslipoprstesnanacinia Protein sarpler from prople with ths condshion hove heen anakysrol, suggsofing thet there is Leficisncy in on angyice System associatucl with [eprct hastabo L'sHeart otlacke . Blood samples ore taken ot regular intervals Conce & dag ow tlorae Bags after the intial chest par). Studits are becrg done to | ent tig bmrohe Sensitive Smarkor’ protetne which will indicate eorlg blockage ih bloock vessels . AsSeseing fetnesg When @ praon is very mack ont of condition thine cam be a serious inerease ithe abound oF Protesn jh urike, which ean be detected by tuo demensional electra phoram - As wolk aa being a poitter to lack of phys ical Fithau, Phereasrh protech content jh the Unie Con alee be @ possibe indbicador of the ohtet of Lahetes .