Tr0Pt43 leaf specific expression MSA F3N114.13—\_ raacie2t low ality transporter Te _-—~ expressed primary in beat high afinity transooner ‘expressed ony in root —— ‘epidermis and conex leaf specific eroression | To14s0 Sy, se r20K19.22 ‘T31P16.170 eae towatinyareporer—‘rassea.t3 exprescedin vascular eypressee primarily eat seve of roots and leaves m ” — 100 Fig 1. Dendogram showing the amino acid sequence relation ship between different Arabidopsis sulfate transporters. The sequences are referenced In Table 3, are indicated by their genomic locus designation. The cencogram was prepared using the GCG, Inc. program PAUPSearch. The properties of trans- porters that have been studied are indicated, This information references the work of Takahashi et al., (1999a; 1999p; 2000),Ne we he ©. Qo 0 - SULFURYLASE tyCOP-0-8-0- ong-o 7 Sot =I OO” +PPi 6. OH OH ‘sulfate ATP. APS 2GSH wi Ye =< ‘cy Kase REDUCTASE 4 208. o=s 4:¢-:0-0-8-0- a = O60 o oH 8 opo paps Fig 2. Reactions catalyzed by ATP sulfurylase, APS reductase and APS kinase. APS AMP APR-SH APR-S-SO3° cst ‘APR-S-SG S03” Fig 3. Proposed reaction mechanism for the catalytic cycle of APS reductase. The model is based on the work of Weber et al, (2000).