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  • Bacterial Culture - Is A Gold Standard Method For Demonstration of Organism Using Culture Media

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    Prativa Rajbhandari
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    Bacterial culture is a standard method for demonstrating bacterial growth using culture media, which provides an artificial environment simulating natural conditions for bacteria to grow. Basic constituents of culture media include water, electrolytes, peptone (a mixture of partially digested proteins, amino acids, inorganic salts, and growth factors), meat extract (containing protein breakdown products, carbohydrates, and inorganic salts), and optionally blood or serum to enrich the media. Agar, obtained from seaweeds and used at a 2-3% concentration, is also included to solidify the culture media due to its high gelling strength and ability to melt at 98°C and solidify at 42°C.

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    Bacterial Culture

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    Bacterial culture is a standard method for demonstrating bacterial growth using culture media, which provides an artificial environment simulating natural conditions for bacteria to grow. Basic constituents of culture media include water, electrolytes, peptone (a mixture of partially digested proteins, amino acids, inorganic salts, and growth factors), meat extract (containing protein breakdown products, carbohydrates, and inorganic salts), and optionally blood or serum to enrich the media. Agar, obtained from seaweeds and used at a 2-3% concentration, is also included to solidify the culture media due to its high gelling strength and ability to melt at 98°C and solidify at 42°C.

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    3 views1 page

    Bacterial Culture - Is A Gold Standard Method For Demonstration of Organism Using Culture Media

    Uploaded by

    Prativa Rajbhandari
    Bacterial culture is a standard method for demonstrating bacterial growth using culture media, which provides an artificial environment simulating natural conditions for bacteria to grow. Basic constituents of culture media include water, electrolytes, peptone (a mixture of partially digested proteins, amino acids, inorganic salts, and growth factors), meat extract (containing protein breakdown products, carbohydrates, and inorganic salts), and optionally blood or serum to enrich the media. Agar, obtained from seaweeds and used at a 2-3% concentration, is also included to solidify the culture media due to its high gelling strength and ability to melt at 98°C and solidify at 42°C.

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    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
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    of 1

    Bacterial Culture

    -is a gold standard method for demonstration of organism using


    culture media
    Culture Media
    -it gives an artificial environment stimulating natural condition
    necessary for bacterial growth
    Basic constituents of culture media
    a) Water- is hydrogen and oxygen source
    b) Electrolytes-e.g. NaCl etc
    c) Peptone- is a complex mixture of partially digested protein
    -it consists of proteases, polypeptide, AAs and inorganic salts
    (phosphorus, K, Mg) and accessory growth factor (riboflavin)
    d) Meat Extract- contains protein degradation products,
    carbohydrates and inorganic salts.
    e) Blood / Serum- 5-10% defibrinated sheep blood is used
    - used for enriching culture media
    f) Agar- obtained for sea weeds
    -its chief constituent is long chain polysaccharide
    - Concentration used 2-3%
    -used to solidify culture media because of its high gelling strength
    - Unique character melting at 980C and solidifying at 420C

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