-is a gold standard method for demonstration of organism using
culture media Culture Media -it gives an artificial environment stimulating natural condition necessary for bacterial growth Basic constituents of culture media a) Water- is hydrogen and oxygen source b) Electrolytes-e.g. NaCl etc c) Peptone- is a complex mixture of partially digested protein -it consists of proteases, polypeptide, AAs and inorganic salts (phosphorus, K, Mg) and accessory growth factor (riboflavin) d) Meat Extract- contains protein degradation products, carbohydrates and inorganic salts. e) Blood / Serum- 5-10% defibrinated sheep blood is used - used for enriching culture media f) Agar- obtained for sea weeds -its chief constituent is long chain polysaccharide - Concentration used 2-3% -used to solidify culture media because of its high gelling strength - Unique character melting at 980C and solidifying at 420C