Worksheet 3

Multiple Choice:
1. What force(s) drive protein folding?
a. The entropy of water
b. Hydrophobic interactions
c. IMFs
d. A and B
2. Common obstacles to protein folding do not include:
a. Aggregation of HB regions
b. Non-native disulfide bridge formation
c. High pH environments
d. Isomerization of proline
3. When does the molten globule state form?
a. 1M234
b. 12M 3 4
c. 123M4
d. M1234
4. Prions (Proteinaceous Infectious Only) do not
a. Allow higher B-sheet content which spreads to other proteins
b. Can lead to formation of amyloid fibrils
c. Cause Alzheimers disease
d. Cause proteins to completely unfold
5. Chaperonins are
a. Non-specific and recognize exposed HB patches
b. Prevent aggregation in response to high heat levels
c. All of the above
d. Necessary for all protein folding
6. With site directed mutagenesis, we can maintain protein stability via
a. Disulfide bridges
b. GP or A
c. N-terminus with more (-) charged a.a. and C-terminus with (+) a.a.
d. Hydrophobicity in the core cavity
e. All of the above
Short Response:
7. Protein folding is statistically impossible if it is completely ____________.
This conundrum is known as ___________________.
8. How did Anfinsen solve this conundrum? (describe his experiment and
give conclusions):

9. Circle the correct answer(s)

a. GroEL has 7 subunits with apical (alpha/beta), intermediate
(alpha/beta), equatorial (alpha/beta).
b. Trp bound to recognition helices open HTH dimers (25/29/34/38) A
apart for proper DNA bidning

10.GroES is made of _____________&________________&________________

11.GroEL never has 2 GroES units bout t it because of ____________ inhibiton.
12.CAP (catabolic gene activating protein)
a. Has what motif?______________
b. Functions as ______#____mer
c. Activated by ___________ (allosteric effector)
d. ________bonds between protein and DNA bps.
13.Draw the Zn finger
a. Label the a.a.s that stabilize the Zn metal, N-terminal and Cterminal

14.Leu Zipper
a. What motif?________________
b. Has a _________ repeat.
c. Almost every 4th residue is _____________
d. ________________ core is a major contributor to stability
e. Distal (basic/acidic) region on each helix reacts with the
(major/minor) groove, so it likely possesses what 2 a.a.s?
_____________
15.T cell receptors recognize __________ proteins when a(n) _____________ is
presented on it.
16.IgG is a ____#_____mer: with ___ light chains and 2 _________
a. Ig fold has a _______________ motif
b. Each light chain contains ____ Ig Folds, and each heavy chain
contains ___ Ig folds
c. Distorted conformations are stabilized by ______ bonds.
d. Antigen recognition loops are located at the __-terminus, where it is
highly variable
e. Antigens bind to the loops via ____bonds
f. Each Ig recognizes ___ antigen
g. Different B cells produce different ______
h. Papain cleaves the ______ region.