BioEdit version 7.2.

5 (12/11/2013) Restriction Mapping Utility

(c)1998, Tom Hall
gb|CP012712.1|:845921-847621 Geobacillus thermoglucosidasius strain DSM 2542, co
mplete genome Restriction Map
2/2/2016 3:08:52 PM
1701 base pairs
Translations: none
Restriction Enzyme Map:
1
ATGAAAAAATGTGATGTAATGGCACAAGTAGAACAAAAGTTGAAAGAAATATATGGACAGGAATACAAAGAG
GAATATGC 80
1
TACTTTTTTACACTACATTACCGTGTTCATCTTGTTTTCAACTTTCTTTATATACCTGTCCTTATGTTTCTC
CTTATACG 80
TspDTI
MnlI

81
AGTGGCATTCCGCTCTTTCATCGAGCGTTGGGAAGCAAAACAATGGCGAAAAAACGAGCCGCTGTCAGAGAA
GAATGTTT 160
81
TCACCGTAAGGCGAGAAAGTAGCTCGCAACCCTTCGTTTTGTTACCGCTTTTTTGCTCGGCGACAGTCTCTT
CTTACAAA 160
BsmI
BsrBI
Hin4I
MspA1I
Hin4I
TspRI
BtsI
TspDTI

161
ATTTAATCACGTATGGTGACAGCATTTTTGAAAAAGGGAAACCAGCGTTAGAGACACTGCATCGATTTTTGA
AGGAGCAA 240
161
TAAATTAGTGCATACCACTGTCGTAAAAACTTTTTCCCTTTGGTCGCAATCTCTGTGACGTAGCTAAAAACT
TCCTCGTT 240
MboII
BsaAI
HphI
Hin4I
BtsI
TspRI
SfaNI
Hin4I
Hin4I
ClaI
Hin4I
BsmAI

241
GTAGGCGACATCATTACAGATGTCCACTTGCTTCCGATGTTTCCATATACATCCGATGACGGTTTTTCAGTG
ACAGATTA 320
241
CATCCGCTGTAGTAATGTCTACAGGTGAACGAAGGCTACAAAGGTATATGTAGGCTACTGCCAAAAAGTCAC
TGTCTAAT 320
Hpy8I
FokI
BstF5I
Hin4I
TspRI
Hin4I
FokI

TspDTI

321
TCGGAAAATTCATCCGAAACTTGGCGGTTGGGAACAAATTGAAGCGATGGCGGCGGATTATCGTCTCATGTT
TGACTTTG 400
321
AGCCTTTTAAGTAGGCTTTGAACCGCCAACCCTTGTTTAACTTCGCTACCGCCGCCTAATAGCAGAGTACAA
ACTGAAAC 400
ApoI
Hin4I
MwoI
E
ciI
Tth111I
BstF5I
BslI
HpyF10VI
BsmBI
Hin4I
Bs
mAI

401
TCGCTAATCATATTTCGAAATCGAGCGAATGGTTTCAGCGGTATTTAGCGGACGATCCAAAATATGAGCATT
ATTTTATT 480
401
AGCGATTAGTATAAAGCTTTAGCTCGCTTACCAAAGTCGCCATAAATCGCCTGCTAGGTTTTATACTCGTAA
TAAAATAA 480
BstBI
XmnI
MspA1I
AlwI
TspGWI

481
CCGAAAGATCCGTCTTTTGATACAAGCAACGTCATTCGTCCGCGCACGACACCGTTGTTCCATGAGTATCAA
GGGATTCA 560
481
GGCTTTCTAGGCAGAAAACTATGTTCGTTGCAGTAAGCAGGCGCGTGCTGTGGCAACAAGGTACTCATAGTT
CCCTAAGT 560
AlwI
TspD
TI
BstYI

561
TCGGGTGAAAACGGCTTGGACAACATTTAGCGAAGATCAAATAGATTTGAATTTCCGGCATTTCCCTGTGCT
ATTAGAAA 640
561
AGCCCACTTTTGCCGAACCTGTTGTAAATCGCTTCTAGTTTATCTAAACTTAAAGGCCGTAAAGGGACACGA
TAATCTTT 640
HphI
BceAI
MboII
ApoI

641
TGACCGATATTTTGCTTGAGTATGCGCATAGACACGGCACGAGCATCCGGTTAGATGCGATCGGCTTCATTT
GGAAGGAA 720
641
ACTGGCTATAAAACGAACTCATACGCGTATCTGTGCCGTGCTCGTAGGCCAATCTACGCTAGCCGAAGTAAA
CCTTCCTT 720
SmlI
FspAI
BpuEI BstF5I
SfaNI
BsiEI
BbvI
TaqII FspI
BssSI
BsaWI
TspDTI

FokI

SfaNI

PvuI
BceAI

721
TCTGGTACAAGCTGCATGCACTTGCCGCAAACGCATGCAATTATTCAGTTATGGCGCCTTCTTCTCGACTAT
TTTCAGCC 800
721
AGACCATGTTCGACGTACGTGAACGGCGTTTGCGTACGTTAATAAGTCAATACCGCGGAAGAAGAGCTGATA
AAAGTCGG 800
Cac8I
MwoI MwoI
NspI
MboII
Hpy
188III
NspI
HpyF10VI Cac8I
BanI
SphI

HpyF10VI
SphI

KasI
BsaHI
NarI
NlaIV
SfoI
BbeI
HaeII

801
GAACACGAATATCATTACAGAAACGAACGTGCCGCATGATGAAAATATTCGTTATTTTGGTAATGGGATGAA
CGAGGCGC 880
801
CTTGTGCTTATAGTAATGTCTTTGCTTGCACGGCGTACTACTTTTATAAGCAATAAAACCATTACCCTACTT
GCTCCGCG 880
XmnI
TspDTI
Mnl
I
FokI
SspI
BstF5I

881
ATATGGTTTACCAATTTTCTTTGCCGCCGCTCGTGTTATATACGTTTACGGTCCATCATTCTCGCAAGTTGA
CAGAGTGG 960
881
TATACCAAATGGTTAAAAGAAACGGCGGCGAGCACAATATATGCAAATGCCAGGTAGTAAGAGCGTTCAACT
GTCTCACC 960
NdeI
Hpy8I
BsrBI
Hpy8I
HincII
TspDTI
BssSI
Hpy8I

961

GCGAAAACGATTGATAAAGTTTCCGACAGCGCTACGTATTTTAACTTTTTAGCGAGCCACGACGGAATCGGC

ATGCGTCC 1040
961
CGCTTTTGCTAACTATTTCAAAGGCTGTCGCGATGCATAAAATTGAAAAATCGCTCGGTGCTGCCTTAGCCG
TACGCAGG 1040
AfeI
MmeI
Cac8I
HgaI
Cac8I
HaeII
NspI
BsaAI
SphI
SnaBI
TspGWI

1041
GACAGAAGGGATTTTGACGGAAGAAGAGAAACAACTGCTTGTGGACAAAGTATTACAAAACGGTGGGAAAGT
GTCCTATA 1120
1041
CTGTCTTCCCTAAAACTGCCTTCTTCTCTTTGTTGACGAACACCTGTTTCATAATGTTTTGCCACCCTTTCA
CAGGATAT 1120
BslI
EarI
TspGWI
Hpy8I
MmeI

MboII
MboII

1121
AAACGAACACGGATGGCAGCCAATCGGTGTATGAGCTGAATATTAATTATTTTGATGCGCTTATTAACAAAG
GTGTCGAT 1200
1121
TTTGCTTGTGCCTACCGTCGGTTAGCCACATACTCGACTTATAATTAATAAAACTACGCGAATAATTGTTTC
CACAGCTA 1200
BstF5I
BbvI
SspI
TspGWI
FokI

AseI
SfaNI

1201
GTGACCGAGCAGCAGCAAGTTCAAAAAATGCTTGCAGCTCATGCGATTTTGTTATCGGTCATTGGTGTTCCG
GCGATTTA 1280
1201
CACTGGCTCGTCGTCGTTCAAGTTTTTTACGAACGTCGAGTACGCTAAAACAATAGCCAGTAACCACAAGGC
CGCTAAAT 1280
TaqII
Cac8I
BstAPI
Hin
4I
BbvI
MwoI BbvI
Hi
n4I
BbvI
HpyF10VI
TaqII

1281

TTATCACTCTTTACTCGGTTCGCGCAATGATTATAAAGGGCTGGAACAATCGGGAATTCCGCGCCGGATAAA

TCGCGAAA 1360
1281
AATAGTGAGAAATGAGCCAAGCGCGTTACTAATATTTCCCGACCTTGTTAGCCCTTAAGGCGCGGCCTATTT
AGCGCTTT 1360
Hin4I
BsrDI
Hpy188III
Hpy188III
Hin4I
PsiI
ApoI
NruI
EcoRI

1361
AACTGGAGTACAGCCAAATTGTTCGTGAACTACAAACGAATTCTCGGCGCTGCGCGATTTTCCGTGGATTGA
AACGAATG 1440
1361
TTGACCTCATGTCGGTTTAACAAGCACTTGATGTTTGCTTAAGAGCCGCGACGCGCTAAAAGGCACCTAACT
TTGCTTAC 1440
BsrI
BpmI
BbvI
HaeII
BsaJI
FokI EciI
TatI
Eco57MI
ApoI
TspGWI
BtgI
Hpy188III

EcoRI

Hpy8I

1441
ATCGCCATCCGCCGCACACAATCGGCATTTTCCCCGTACGCCCCGCAGCAAGTGATCGAACTAGGCGATCAC
GTATTTGC 1520
1441
TAGCGGTAGGCGGCGTGTGTTAGCCGTAAAAGGGGCATGCGGGGCGTCGTTCACTAGCTTGATCCGCTAGTG
CATAAACG 1520
BsaBI
BsiWI
FauI
BbvI
BceA
I
BsaAI
BstF5I

1521
CGTAAAAAGAACGAACGAACAGACTGGCGACTCGATATATTTTGTTGTCAACGTGACACCAGCCGAAATTGC
TGTCGATT 1600
1521
GCATTTTTCTTGCTTGCTTGTCTGACCGCTGAGCTATATAAAACAACAGTTGCACTGTGGTCGGCTTTAACG
ACAGCTAA 1600
MlyI
HincII
Mw
oI
PleI
Hpy8I
HpyF10VI
BsrI

1601
TAGGAGTAAGCGGGGAAGATTTATGGACGGGAGAAAAAATAAACAGATCGTTCCGGTTGCTTCCGTATCAGT
TTGTGTGG 1680
1601
ATCCTCATTCGCCCCTTCTAAATACCTGCCCTCTTTTTTATTTGTCTAGCAAGGCCAACGAAGGCATAGTCA
AACACACC 1680
FauI
MboII
TspGWI
T

aqII

Hin4I
BsaWI
BsaXI

1681
1681

GTGAAGGAGAAATTGTCTTAA
CACTTCCTCTTTAACAGAATT
HphI

1701
1701

Restriction table:
Enzyme
Recognition
frequency Positions
__________________________________________________________________________
AfeI
AGC'GCT
1
991
AlwI
GGATCnnnn'n_
2
449, 482
ApoI
r'AATT_y
4
327, 610, 1335, 1399
AseI
AT'TA_AT
1
1164
BanI
G'GyrC_C
1
774
BbeI
G_GCGC'C
1
778
BbvI
GCAGCnnnnnnnn'nnnn_
7
719, 1149, 1222, 1225, 1247,
1397
1498
BceAI
ACGGCnnnnnnnnnnnn'nn_
3
588, 691, 1505
BpmI
CTGGAGnnnnnnnnnnnnnn_nn'
1
1385
BpuEI
CTTGAGnnnnnnnnnnnnnn_nn'
1
677
BsaAI
yAC'GTr
3
171, 996, 1513
BsaBI
GATnn'nnATC
1
1445
BsaHI
Gr'CG_yC
1
775
BsaJI
C'CnnG_G
1
1423
BsaWI
w'CCGG_w
2
687, 1653
BsaXI
ACnnnnnCTCCnnnnnnn_nnn'
1
1679
BsiEI
CG_ry'CG
1
702
BsiWI
C'GTAC_G
1
1476
BslI
CCnn_nnn'nnGG
2
341, 1046
BsmI
GAATG_Cn'
1
86
BsmAI
GTCTCn'nnnn_
2
206, 389
BsmBI
CGTCTCn'nnnn_
1
389
BsrI
ACTG_Gn'
2
1368, 1549
BsrBI
CCG'CTC
2
93, 910
BsrDI
GCAATG_nn'
1
1312
BssSI
C'ACGA_G
2
679, 911
BstAPI
GCAn_nnn'nTGC
1
1241
BstBI
TT'CG_AA
1
416
BstF5I
GGATG_nn'
6
290, 331, 684, 873, 1138, 144
6
BstYI
r'GATC_y
1
487
BtgI
C'CryG_G
1
1423
BtsI
GCAGTG_nn'
2
87, 215
Cac8I
GCn'nGC
5
737, 756, 1015, 1034, 1233
ClaI
AT'CG_AT
1
223
EarI
CTCTTCn'nnn_
1
1059
EciI
GGCGGAnnnnnnnnn_nn'
2
389, 1439
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
1
1385
EcoRI
G'AATT_C
2
1335, 1399
FauI
CCCGCnnnn'nn_
2
1491, 1604
FokI
GGATGnnnnnnnnn'nnnn_
6
277, 318, 671, 880, 1145, 143

3
FspI
FspAI
HaeII
HgaI
Hin4I

TGC'GCA
rTGC'GCAy
r_GCGC'y
GACGCnnnnn'nnnnn_
GAynnnnnvTCnnnnnnnn_nnnnn'

1
1
3
1
8

Hin4I

GAbnnnnnrTCnnnnnnnn_nnnnn'

HincII
HphI
Hpy8I
8

GTy'rAC
GGTGAnnnnnnn_n'
GTn'nAC

2
3
7

666
666
778, 993, 1411
1025
128, 205, 237, 304, 336, 1267
1299, 1679
160, 205, 237, 304, 336, 1267
1299
951, 1570
188, 577, 1693
265, 889, 927, 951, 1084, 138

Hpy188III
HpyF10VI
KasI
MboII
28
MlyI
MmeI
MnlI
MspA1I
MwoI
NarI
NdeI
NlaIV
NruI
NspI
PleI
PsiI
PvuI
SfaNI
SfoI
SmlI
SnaBI
SphI
SspI
TaqII
TaqII
TatI
TspDTI
84
TspGWI
1653
TspRI
Tth111I
XmnI

TC'nn_GA
GCn_nnnnn'nGC
G'GCGC_C
GAAGAnnnnnnn_n'

4
5
1
6

1570
785, 1332, 1355, 1385
372, 746, 752, 1242, 1590
774
163, 605, 773, 1073, 1076, 16

GAGTCnnnnn'
TCCrACnnnnnnnnnnnnnnnnnn_nn'
CCTCnnnnnn_n'
CmG'CkG
GCnn_nnn'nnGC
GG'CG_CC
CA'TA_TG
GGn'nCC
TCG'CGA
r_CATG'y
GAGTCnnnn'n_
TTA'TAA
CG_AT'CG
GCATCnnnnn'nnnn_
GGC'GCC
C'TyrA_G
TAC'GTA
G_CATG'C
AAT'ATT
GACCGAnnnnnnnnn_nn'
CACCCAnnnnnnnnn_nn'
w'GTAC_w
ATGAAnnnnnnnnn_nn'

1
2
2
2
5
1
1
1
1
3
1
1
1
4
1
1
1
3
2
3
1
1
7

1544
1008, 1064
64, 868
142, 439
371, 745, 751, 1241, 1589
775
882
776
1356
739, 758, 1036
1544
1314
702
229, 693, 685, 1165
776
656
996
739, 758, 1036
847, 1162
659, 1220, 1246
1669
1368
17, 88, 320, 548, 697, 855, 8

ACGGAnnnnnnnnn_nn'

480, 1038, 1073, 1145, 1412,

_nnCAsTGnn'
GACn'n_nGTC
GAAnn'nnTTC

3
1
2

87, 222, 315

398
432, 846

Enzymes that cut five or fewer times

Enzyme
Recognition
frequency Positions
__________________________________________________________________________
AfeI
AGC'GCT
1
991
AlwI
GGATCnnnn'n_
2
449, 482
ApoI
r'AATT_y
4
327, 610, 1335, 1399
AseI
AT'TA_AT
1
1164
BanI
G'GyrC_C
1
774
BbeI
G_GCGC'C
1
778

BceAI
BpmI
BpuEI
BsaAI
BsaBI
BsaHI
BsaJI
BsaWI
BsaXI
BsiEI
BsiWI
BslI
BsmI
BsmAI
BsmBI
BsrI
BsrBI
BsrDI
BssSI
BstAPI
BstBI
BstYI
BtgI
BtsI
Cac8I
ClaI
EarI
EciI
Eco57MI
EcoRI
FauI
FspI
FspAI
HaeII
HgaI
HincII
HphI
Hpy188III
HpyF10VI
KasI
MlyI
MmeI
MnlI
MspA1I
MwoI
NarI
NdeI
NlaIV
NruI
NspI
PleI
PsiI
PvuI
SfaNI
SfoI
SmlI
SnaBI
SphI
SspI
TaqII

ACGGCnnnnnnnnnnnn'nn_
CTGGAGnnnnnnnnnnnnnn_nn'
CTTGAGnnnnnnnnnnnnnn_nn'
yAC'GTr
GATnn'nnATC
Gr'CG_yC
C'CnnG_G
w'CCGG_w
ACnnnnnCTCCnnnnnnn_nnn'
CG_ry'CG
C'GTAC_G
CCnn_nnn'nnGG
GAATG_Cn'
GTCTCn'nnnn_
CGTCTCn'nnnn_
ACTG_Gn'
CCG'CTC
GCAATG_nn'
C'ACGA_G
GCAn_nnn'nTGC
TT'CG_AA
r'GATC_y
C'CryG_G
GCAGTG_nn'
GCn'nGC
AT'CG_AT
CTCTTCn'nnn_
GGCGGAnnnnnnnnn_nn'
CTGrAGnnnnnnnnnnnnnn_nn'
G'AATT_C
CCCGCnnnn'nn_
TGC'GCA
rTGC'GCAy
r_GCGC'y
GACGCnnnnn'nnnnn_
GTy'rAC
GGTGAnnnnnnn_n'
TC'nn_GA
GCn_nnnnn'nGC
G'GCGC_C
GAGTCnnnnn'
TCCrACnnnnnnnnnnnnnnnnnn_nn'
CCTCnnnnnn_n'
CmG'CkG
GCnn_nnn'nnGC
GG'CG_CC
CA'TA_TG
GGn'nCC
TCG'CGA
r_CATG'y
GAGTCnnnn'n_
TTA'TAA
CG_AT'CG
GCATCnnnnn'nnnn_
GGC'GCC
C'TyrA_G
TAC'GTA
G_CATG'C
AAT'ATT
GACCGAnnnnnnnnn_nn'

3
1
1
3
1
1
1
2
1
1
1
2
1
2
1
2
2
1
2
1
1
1
1
2
5
1
1
2
1
2
2
1
1
3
1
2
3
4
5
1
1
2
2
2
5
1
1
1
1
3
1
1
1
4
1
1
1
3
2
3

588, 691, 1505

1385
677
171, 996, 1513
1445
775
1423
687, 1653
1679
702
1476
341, 1046
86
206, 389
389
1368, 1549
93, 910
1312
679, 911
1241
416
487
1423
87, 215
737, 756, 1015, 1034, 1233
223
1059
389, 1439
1385
1335, 1399
1491, 1604
666
666
778, 993, 1411
1025
951, 1570
188, 577, 1693
785, 1332, 1355, 1385
372, 746, 752, 1242, 1590
774
1544
1008, 1064
64, 868
142, 439
371, 745, 751, 1241, 1589
775
882
776
1356
739, 758, 1036
1544
1314
702
229, 693, 685, 1165
776
656
996
739, 758, 1036
847, 1162
659, 1220, 1246

TaqII
TatI
TspRI
Tth111I
XmnI

CACCCAnnnnnnnnn_nn'
w'GTAC_w
_nnCAsTGnn'
GACn'n_nGTC
GAAnn'nnTTC

1
1
3
1
2

1669
1368
87, 222, 315
398
432, 846

Position
Enzyme(s)
__________________________________________________________________________
17
TspDTI
ATGAAnnnnnnnnn_nn'
64
MnlI
CCTCnnnnnn_n'
86
BsmI
GAATG_Cn'
87
TspRI
_nnCAsTGnn'
87
BtsI
GCAGTG_nn'
88
TspDTI
ATGAAnnnnnnnnn_nn'
93
BsrBI
CCG'CTC
128
Hin4I
GAynnnnnvTCnnnnnnnn_nnnnn'
142
MspA1I
CmG'CkG
160
Hin4I
GAbnnnnnrTCnnnnnnnn_nnnnn'
163
MboII
GAAGAnnnnnnn_n'
171
BsaAI
yAC'GTr
188
HphI
GGTGAnnnnnnn_n'
205
Hin4I
GAynnnnnvTCnnnnnnnn_nnnnn'
205
Hin4I
GAbnnnnnrTCnnnnnnnn_nnnnn'
206
BsmAI
GTCTCn'nnnn_
215
BtsI
GCAGTG_nn'
222
TspRI
_nnCAsTGnn'
223
ClaI
AT'CG_AT
229
SfaNI
GCATCnnnnn'nnnn_
237
Hin4I
GAynnnnnvTCnnnnnnnn_nnnnn'
237
Hin4I
GAbnnnnnrTCnnnnnnnn_nnnnn'
265
Hpy8I
GTn'nAC
277
FokI
GGATGnnnnnnnnn'nnnn_
290
BstF5I
GGATG_nn'
304
Hin4I
GAynnnnnvTCnnnnnnnn_nnnnn'
304
Hin4I
GAbnnnnnrTCnnnnnnnn_nnnnn'
315
TspRI
_nnCAsTGnn'
318
FokI
GGATGnnnnnnnnn'nnnn_
320
TspDTI
ATGAAnnnnnnnnn_nn'
327
ApoI
r'AATT_y
331
BstF5I
GGATG_nn'
336
Hin4I
GAynnnnnvTCnnnnnnnn_nnnnn'
336
Hin4I
GAbnnnnnrTCnnnnnnnn_nnnnn'
341
BslI
CCnn_nnn'nnGG
371
MwoI
GCnn_nnn'nnGC
372
HpyF10VI
GCn_nnnnn'nGC
389
EciI
GGCGGAnnnnnnnnn_nn'
389
BsmBI
CGTCTCn'nnnn_
389
BsmAI
GTCTCn'nnnn_
398
Tth111I
GACn'n_nGTC
416
BstBI
TT'CG_AA
432
XmnI
GAAnn'nnTTC
439
MspA1I
CmG'CkG
449
AlwI
GGATCnnnn'n_
480
TspGWI
ACGGAnnnnnnnnn_nn'
482
AlwI
GGATCnnnn'n_
487
BstYI
r'GATC_y
548
TspDTI
ATGAAnnnnnnnnn_nn'
577
HphI
GGTGAnnnnnnn_n'
588
BceAI
ACGGCnnnnnnnnnnnn'nn_

605
610
656
659
666
666
671
677
679
684
685
687
691
693
697
702
702
719
737
739
739
745
746
751
752
756
758
758
773
774
774
775
775
776
776
778
778
785
846
847
855
868
873
880
882
884
889
910
911
927
951
951
991
993
996
996
1008
1015
1025
1034

MboII
ApoI
SmlI
TaqII
FspAI
FspI
FokI
BpuEI
BssSI
BstF5I
SfaNI
BsaWI
BceAI
SfaNI
TspDTI
BsiEI
PvuI
BbvI
Cac8I
NspI
SphI
MwoI
HpyF10VI
MwoI
HpyF10VI
Cac8I
NspI
SphI
MboII
BanI
KasI
BsaHI
NarI
NlaIV
SfoI
BbeI
HaeII
Hpy188III
XmnI
SspI
TspDTI
MnlI
BstF5I
FokI
NdeI
TspDTI
Hpy8I
BsrBI
BssSI
Hpy8I
HincII
Hpy8I
AfeI
HaeII
BsaAI
SnaBI
MmeI
Cac8I
HgaI
Cac8I

GAAGAnnnnnnn_n'
r'AATT_y
C'TyrA_G
GACCGAnnnnnnnnn_nn'
rTGC'GCAy
TGC'GCA
GGATGnnnnnnnnn'nnnn_
CTTGAGnnnnnnnnnnnnnn_nn'
C'ACGA_G
GGATG_nn'
GCATCnnnnn'nnnn_
w'CCGG_w
ACGGCnnnnnnnnnnnn'nn_
GCATCnnnnn'nnnn_
ATGAAnnnnnnnnn_nn'
CG_ry'CG
CG_AT'CG
GCAGCnnnnnnnn'nnnn_
GCn'nGC
r_CATG'y
G_CATG'C
GCnn_nnn'nnGC
GCn_nnnnn'nGC
GCnn_nnn'nnGC
GCn_nnnnn'nGC
GCn'nGC
r_CATG'y
G_CATG'C
GAAGAnnnnnnn_n'
G'GyrC_C
G'GCGC_C
Gr'CG_yC
GG'CG_CC
GGn'nCC
GGC'GCC
G_GCGC'C
r_GCGC'y
TC'nn_GA
GAAnn'nnTTC
AAT'ATT
ATGAAnnnnnnnnn_nn'
CCTCnnnnnn_n'
GGATG_nn'
GGATGnnnnnnnnn'nnnn_
CA'TA_TG
ATGAAnnnnnnnnn_nn'
GTn'nAC
CCG'CTC
C'ACGA_G
GTn'nAC
GTy'rAC
GTn'nAC
AGC'GCT
r_GCGC'y
yAC'GTr
TAC'GTA
TCCrACnnnnnnnnnnnnnnnnnn_nn'
GCn'nGC
GACGCnnnnn'nnnnn_
GCn'nGC

1036
1036
1038
1046
1059
1064
1073
1073
1076
1084
1138
1145
1145
1149
1162
1164
1165
1220
1222
1225
1233
1241
1241
1242
1246
1247
1267
1267
1299
1299
1312
1314
1332
1335
1335
1355
1356
1368
1368
1385
1385
1385
1388
1397
1399
1399
1411
1412
1423
1423
1433
1439
1445
1446
1476
1491
1498
1505
1513
1544

NspI
SphI
TspGWI
BslI
EarI
MmeI
TspGWI
MboII
MboII
Hpy8I
BstF5I
TspGWI
FokI
BbvI
SspI
AseI
SfaNI
TaqII
BbvI
BbvI
Cac8I
BstAPI
MwoI
HpyF10VI
TaqII
BbvI
Hin4I
Hin4I
Hin4I
Hin4I
BsrDI
PsiI
Hpy188III
ApoI
EcoRI
Hpy188III
NruI
BsrI
TatI
BpmI
Eco57MI
Hpy188III
Hpy8I
BbvI
ApoI
EcoRI
HaeII
TspGWI
BsaJI
BtgI
FokI
EciI
BsaBI
BstF5I
BsiWI
FauI
BbvI
BceAI
BsaAI
MlyI

r_CATG'y
G_CATG'C
ACGGAnnnnnnnnn_nn'
CCnn_nnn'nnGG
CTCTTCn'nnn_
TCCrACnnnnnnnnnnnnnnnnnn_nn'
ACGGAnnnnnnnnn_nn'
GAAGAnnnnnnn_n'
GAAGAnnnnnnn_n'
GTn'nAC
GGATG_nn'
ACGGAnnnnnnnnn_nn'
GGATGnnnnnnnnn'nnnn_
GCAGCnnnnnnnn'nnnn_
AAT'ATT
AT'TA_AT
GCATCnnnnn'nnnn_
GACCGAnnnnnnnnn_nn'
GCAGCnnnnnnnn'nnnn_
GCAGCnnnnnnnn'nnnn_
GCn'nGC
GCAn_nnn'nTGC
GCnn_nnn'nnGC
GCn_nnnnn'nGC
GACCGAnnnnnnnnn_nn'
GCAGCnnnnnnnn'nnnn_
GAynnnnnvTCnnnnnnnn_nnnnn'
GAbnnnnnrTCnnnnnnnn_nnnnn'
GAynnnnnvTCnnnnnnnn_nnnnn'
GAbnnnnnrTCnnnnnnnn_nnnnn'
GCAATG_nn'
TTA'TAA
TC'nn_GA
r'AATT_y
G'AATT_C
TC'nn_GA
TCG'CGA
ACTG_Gn'
w'GTAC_w
CTGGAGnnnnnnnnnnnnnn_nn'
CTGrAGnnnnnnnnnnnnnn_nn'
TC'nn_GA
GTn'nAC
GCAGCnnnnnnnn'nnnn_
r'AATT_y
G'AATT_C
r_GCGC'y
ACGGAnnnnnnnnn_nn'
C'CnnG_G
C'CryG_G
GGATGnnnnnnnnn'nnnn_
GGCGGAnnnnnnnnn_nn'
GATnn'nnATC
GGATG_nn'
C'GTAC_G
CCCGCnnnn'nn_
GCAGCnnnnnnnn'nnnn_
ACGGCnnnnnnnnnnnn'nn_
yAC'GTr
GAGTCnnnnn'

1544
1549
1570
1570
1589
1590
1604
1628
1653
1653
1669
1679
1679
1693

PleI
BsrI
HincII
Hpy8I
MwoI
HpyF10VI
FauI
MboII
TspGWI
BsaWI
TaqII
Hin4I
BsaXI
HphI

GAGTCnnnn'n_
ACTG_Gn'
GTy'rAC
GTn'nAC
GCnn_nnn'nnGC
GCn_nnnnn'nGC
CCCGCnnnn'nn_
GAAGAnnnnnnn_n'
ACGGAnnnnnnnnn_nn'
w'CCGG_w
CACCCAnnnnnnnnn_nn'
GAynnnnnvTCnnnnnnnn_nnnnn'
ACnnnnnCTCCnnnnnnn_nnn'
GGTGAnnnnnnn_n'

Enzymes that do not cut:

_________________________________________________________
AarI, AatII, AccI, Acc65I, AclI, AflII, AflIII, AgeI, AhdI, AleI, AloI, AloI
AlwNI, ApaI, ApaLI, AscI, AsiSI, AvaI, AvrII, BaeI, BaeI, BamHI, BanII, BbsI
BbvCI, BcgI, BcgI, BciVI, BclI, BfrBI, BglI, BglII, BlpI, Bme1580I, BmgBI, BmrI
BmtI, BplI, Bpu10I, BsaI, BsaXI, BseMII, BseRI, BseYI, BsgI, BsiHKAI, BsmFI
Bsp1286I, BspCNI, BspEI, BspHI, BspMI, BsrFI, BsrGI, BssHII, BstEII, BstXI
BstZ17I, Bsu36I, DraI, DraIII, DrdI, EaeI, EagI, Eco57I, EcoICRI, EcoNI, EcoO109
I
EcoRV, FalI, FseI, HindIII, HpaI, KpnI, MfeI, MluI, MscI, MslI, NaeI, NcoI, NgoM
IV
NheI, NotI, NsiI, PacI, PciI, PflMI, PmeI, PmlI, PpiI, PpiI, PpuMI, PshAI, PspOM
I
PsrI, PsrI, PstI, PvuII, RsrII, SacI, SacII, SalI, SanDI, SapI, SbfI, ScaI, SexA
I
SfcI, SfiI, SgrAI, SmaI, SpeI, SrfI, StuI, StyI, SwaI, XbaI, XcmI, XhoI, XmaI
ZraI