Exercise 1: Cell Transport Mechanisms and Permeability: Activity 3: Simulating Osmotic Pressure Lab Report

Pre-lab Quiz Results

You scored 100% by answering 4 out of 4 questions correctly.
1. Which of the following is true of osmosis?
You correctly answered: c. It is a type of diffusion.
2. Which of the following occurs when a hypertonic solution is added to cells?
You correctly answered: d. The cells shrink.
3. The variable that affects osmotic pressure is
You correctly answered: a. the concentration of nondiffusing solutes.
4. The net movement of water would be into the cell in a
You correctly answered: b. hypotonic solution.

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Experiment Results
Predict Question:
Predict Question 1: What effect do you think increasing the Na+ Cl- concentration will have?
Your answer : a. increased pressure
Predict Question 2: What do you think will be the pressure result of the current experimental conditions?
Your answer : a. no change in pressure
Stop & Think Questions:
Why do you think there is no pressure change?
You correctly answered: b. Sodium is able to diffuse through the pores.
Why do you think there was no pressure change?
You correctly answered: c. There is no net movement of water because the solute concentrations are the same.
Experiment Data:
Run Number
1
2
3
4
5
6
7

Solute
Na+ ClNa+ ClNa+ ClGlucose
Glucose
Glucose
Albumin
Glucose

MWCO
20
20
50
100
100
200
200
200

Start Conc. L
5.00
10.00
10.00
8.00
8.00
8.00
9.00
0.00

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Pres. L
170
340
0
136
0
0
153
153

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Start Conc. R
0.00
0.00
0.00
0.00
8.00
0.00
0.00
10.00

Pres. R
0
0
0
0
0
0
0
0

Rate
0.0000
0.0000
0.0167
0.0000
0.0000
0.0036
0.0000
0.0044

Post-lab Quiz Results

You scored 100% by answering 4 out of 4 questions correctly.
1. If you double the concentration of a nondiffusible solute that is on one side of a membrane, the osmotic pressure will
You correctly answered: c. double.
2. Which membrane did not allow Na+ Cl- to pass through?
You correctly answered: a. 20 MWCO
3. When a solute is able to diffuse through a membrane
You correctly answered: b. equilibrium is reached.
4. Water diffuses
You correctly answered: c. toward solutes.

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Review Sheet Results

1. Explain the effect that increasing the Na+ Cl- concentration had on osmotic pressure and why it has this effect. How well
did the results compare with your prediction?
Your answer:
Si en primera instancia el NaCl no atravez la membrana de 20MWCOO y por ende el agua y el soluto tenen una presion
osmotica, se puede asumir que al duplicar la concentracion del soluto la presion aumentara.
2. Describe one way in which osmosis is similar to simple diffusion and one way in which it is different.
Your answer:
La difusion simple y la osmosis no necesitan energia celular.
La difusion simple transporta diferentes solutos como los liposolubles y los muy pequeos que se difunden a traves de la
membrana, en cambio la osmosis es una dialisis que solo transporta agua.

3. Solutes are sometimes measured in milliosmoles. Explain the statement, Water chases milliosmoles.
Your answer:
Como el agua va de una mayor concentracion a una menor. Cuando en una parte de la celula ya sea exterior o interior se
acumula una mayor concentracion de soluto, el agua siempre se va a desplazar hacia la mayor concentracion de soluto ya
que tiene que estar en equilibrio y esto se clasifican como soluciones isotonicas, hipertonica e hipotonicas.
4. The conditions were 9 mM albumin in the left beaker and 10 mM glucose in the right beaker with the 200 MWCO
membrane in place. Explain the results. How well did the results compare with your prediction?
Your answer:
Como la glucosa se tranporta en una membrana de 200MWCO este estaria en quilibrio pero la albumina es muy grande
para trasladarse por esta membrana asi que este no afectaria en la presion del otro.
Mi prediccion fue la correcta.

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