CHEM 3380 Sp16 Quiz 5

Name____Key______________

Use correct significant figures in your answers when appropriate.

Radioactivity:
1. (1.5 pts) What are the three main types of emitted radioactivity?
- particles
- particles
- rays

2. (2 pts) Calculate the half-life of an isotope from the following experimental

measurements:
At t = 0, activity is 600 disintegrations per minute (dpm). After 1 hour, the activity
is 400 disintegrations per minute.
you may use the following: ln N/N0 = -t t1/2 = 0.693/
where N0 = number of radioactive atoms present at time t = 0, and N at time t
ln (400/600) = = - (60 minutes) solve for
-0.405 = -(60)
0.00675 =

t1/2 = 0.693/
= 0.693/0.00675
= 102 minutes or if you solved in hours, = 1.7 hours

3. (1.5 pts) In the process of scintillation counting (shown schematically below),

what is the process that gives rise to a signal (i.e. what are the circle,
benzene starred ring, and brown molecule representing?)

-emitter

solvent in
excited state

fluor that
absorbs energy from
solvent and emits
fluorescent signal

Ligand Binding:
4. (1pt) Consider the following ligand binding interaction:
L+M

LM

What are 2 important assumptions made about the binding interaction?

several correct answers see first page of notes from ligand binding

Consider following Scatchard plot:

2.000
1.500

v/[L]
1.000
0.500
0.000
20
0

20

40

60

80

100

v
5. (1 pt) What does the value of the x-intercept mean? the value of n = total
binding sites
6. (2 pts) How would you determine the formation constant for the binding
interaction shown in the Scatchard plot? (What constant from the plot is
equivalent to the formation constant?)
How does this relate to the
dissociation constant?
the slope is equal to -Kf
y intercept = nKf
Kf = 1/ Kd
7. (1 pt) What information does a Hill plot tell you?
it tells you whether you have cooperative binding and whether it is positive or
negative cooperativity