Entamoeba histolytica (causing amoebiasis) cma Optom te e. leone ng othe envionment teres at ‘rere (Ctopreste Pret wt) videos P.O BB De ay rate ticity estat ser cone ‘atu route nective . 48 ProtozoologyMebane ‘Cronin nrg membrane ian ‘aematophagous ayesone Parcels erntton rom Estamos cl antes! commana Untaind prepartons E naea Trephazate Ecol cower Ofepesn Corpus ean Car toeite Puowopeds Baal sate ovement Stugpish Pape Nepal i Ns recta gas wh ewraly Ring ct gor Aedbloodcats RBCs) ——_esions ‘cues cy. veoh ) coe, baci no RBCS “18-80 15-50 ym Precystand nie ost emt Ofosam = arlar yb ake ins la cng Rosaretasie cyanide! incon sre tac tes Sromdaltars Glycogen masses: Giycogen masses: pet fae ‘Supe Ran ane Wat Corps tain ous otis ‘treacle nel ih re ‘react a wih cenit tensome coe hanonores m Ratetcromialten oars shoe 0-20 ft trary 1-35 Intestinal protozoa 49Entamoeba histolytica (causing amoebiasis) (continued) Morphology (continued) Ioaineprparatons E netytca Wri Ect rn, stise ‘syoopen Brown, compet Srsyoanie yew Oflam Cconsiocus ganrty Yoon ingwn cena Nal Nace marian wih ‘cen anesome eto of arose) anos ty ‘Stains by ron homatonyin “= ap Pky pon Se nr 2 ee zane a el ss mer ‘Sonal black central dt Karyosome ec acct @ & ee ae ea me how comin plautt | wa | ee Ta ee oe oat aoa | _ ie ee a sonra : Se ee es Compal rset See toot ‘ecules maybe a 50 ProtozoologyPathology Invasion of the large intestine = seen goer eo tcretine Eee “Tho primary ——_rvasoneh mcsavia cys weet apr ay oneness wi esa “eee pac ir necescassng poston ae Fleseshane? Lag beind-esenson Extrsionin Musclris rucesee lave eit mucosa ‘ecumuaton of aresbae supertalk (Gtr exons of crac Formation Atscrses may cones iri cfebuses Tusa (eres ay dou wth eset enon Deep sare mucosae every perc fxonsion —_{areclyrva esse) ‘age moss eub-minoan, venme sduoaonea Petts Fenore Surordng areca wacton ane Rblatepollratin ‘amass under cedenaaus mucosa with “real scar reco ave ad anosbas Siroundeg gandonaous ese ora min oops, ‘a tries, Bart aca fea tase xtritostinal lesions 23052 Intestinal protozoa 51Entamoeba histolytica (causing amoebiasis) coninaa) ‘Eazsntestnal sions in amoebiasis eens nt Fate hens et Fath hen i se i i a f Ma om = Ot on Patra (Serco Sean en frm at bari oc sures ses SSoming tee eo + regular margins + Amoabae digest pathways ints tissue terte Nee? andthe ‘ie sy +E rng ein ree onon Direc extension Faemaiogenous pread ‘52 ProtozoologyLaboratory diagnosis Diagnosis depends primarily on demonstration of haematophagous trophozoites of E. histolytica in stool samples, aspirates from intestinal and other organs, biopsy material (pinch biopsy at proctoscopy or sigmoidoscopy and surgical biopsy from clsewhere) and in mucus from rectal ulcers. LISA are available for the detection of Einfamoebu antigen and specific E. histolytica lectin antigen in faecal samples. Serology is the method of choice for diagnosis of amoebic liver disease. Facal appearances in ameblcdyeniany ‘Character Nakad ye Faoalatior ‘Almas poset Moos Noten Notaburcat Microscopie 1 Baca Numerous 2 Pie cale Scan, wel present 5. Re hod cl tenn ova | Lge macrophages Notateatre 5 Charcot Lydencysas May be preset bt are rorspacte 6. Howmatohagisropbznes of htaics reser ‘Cure Usual ined retro rosa abort lath ELISA used fo spe enseton FE ois fom ot bephazole. Enee eechophores tom cared tophaente SSanatoratve. Haamatphagous amosberophootes (@) ist mirescopy (Tiere san (HAE sain Notes ‘Vegetative E. histolytica when seen is actively motile and moves purposefully, There are finger-like, clear pseudopodia and Ingested red cells. No nucleus can be seen. Precysts or cysts found in semi-formed or solid stool have typical nuclear characteristics (1-4 nuclei) and glycogen and chromiial bars can be demonstrated Diagnostic tests Polymorph leucocytosis, Examination of stool samples may show cysts and trophozoites of E. histolytica. Serological tests (FAT, ELISA, cellulose acetate precipitin, latex agglutination) but serology is postive in no more than 75% of cases of amoebic colitis Examine aspirated material for F histolytica. Histology of rectal and colon biopsy material Intestinal protozoa 53Other intestinal amoebae Life cycle So. ten (seen fe at 7 "ron envionment inten | Eneystation (exept noi Peete ‘yats vgaave fares fDi) TE enrereon normed sno, epee oe und arose Morphology Unstained ‘Vegetative forms (vephozotee) Evfanoebe ci Enlinex nano ltarosta——Detamcobo——Entanoate —Enumoabeclipor—_Enanocbs ‘nso ‘aa Snover ms ‘Sue um SSB TSE Moat ‘Soa Feyacive ——Veyscive ave | te lite tre ovr ‘Aundart ‘Aountere Peewdopod | Bunt naiy Suni mariy ‘But. cesr_—Leatitecear—Fngve, car Faget, ear Frere, doar wus oe .% E ‘Ahave arr ecto cyt Ingest BCH Noingne RCE Moingned RBC Endopiasm cc Sreearans fo ia rosea ings Ingest Nucleus igo Gene Gen Two,cnleion Garey | tacos = ‘tao ino roe Preys runt up, dacarge ed pares bata ec)Entamoeba coi Endoimax nana Iedamoaba——Dietamaeba “Entamoeba Entamoeba dispar Entamoeba atch ‘aoyea ‘naman ‘Ofeplean ‘With hematoxyin, stars Bushey ae Stain black xcept gycogen as clear area ‘Nusoor Ai sion | PO @ g Merérane Tee Thin Tick Deleate Crromatin on Coase None Soneliras Nore Fin granuies ‘mererane ‘andar otra! Central gran ‘Smal ool Kiem | Oominrgeety Lae Lane! rane ‘Fan natwork | Maybe comin Nochromain —Nociromain Decale fis. No on seen ets Fariass Haass Pathogenicity | Harioss Hrmioss armies Dspued Invasive commensal commensal | comes arta conmer Entamoebs col ests odamooba butschi cysts Entaroebs hstoytealdspar cysts Intestinal protozoa 55