Beta Thearpeutics Startup Co

Fixed Costs
Equipment
Autocalve
Homogeniser
Waters Acqquity UPLC System Instrument with Computer Liquid Chromatograph
-80 C Freezer
4 C Refridgerator
Veriti Thermal Cycler
20 L Microbial Fermenter BIOSTAT
Small Centrifuge
Computer
Microplate Reader (SpectraMax Plus 384 Absorbance Microplate Reader)
Microfuge
Microtitre incubator
Visible Spectrophotometer
Colony Counter
Chairs
Total

Number of Units
1
1
1
1
1
2
1
1
1
1
1
1
1
1
4

Genetic Construct
TMSB4X gene insert within pUC57 vector, 4g (@ $0.35/bp)

Number of Units
1

LIMS 2 - 20 square metre Wet Lab space ($ 1100 p.a/m2)

LIMS 2 - 10 square metre Dry Lab space ($ 750 p.a/m2)
Total

N/A
N/A

Ongoing Rent Cost (per annum)

Beta Thearpeutics Startup Cost Estimations

Supplier
Tuttnauer
Stuart Equipment
Waters Alliance via www.conquerscientific.com
WolfLabs via sales@wolflabs.co.uk
VWR International
Applied Biosystems
Sartorius Stedim
Stuart Equipment
Dell Australia
Beckman Coulter
Bio Rad
Stuart Equipment
Jenway
Stuart Equipment
Lotus Tech (labfurniture.net.au)

Price per Unit (AUD)

Supplier
GenScript

Price per Unit (AUD)

4000.00
2100.00
47363.72
4701.6
2,105.25
4835.70
23196.60
1693.00
799.00
2800.00
240.00
1995.00
3256.00
1015.00
924.00
101024.87

229.95

num)
La Trobe University
La Trobe University

22000.00
7500.00
29500.00

Reagent Costs
Reagents
PCR Reagents (if kit unavailable)
MgCl2(100g)
PBS (100 tablets)
TRIS hydrochloride pH 8.5(100ml)
NaCl (1kg)
Total
Culture Prep
Terrific Broth (Powderv500 g)
One Shot BL21 (DE3)strain Escherichia coli culture kit
Kanamycin Sulphate 25 g
Total

Supplier
Sigma-Aldrich
Sigma-Aldrich
Sigma-Aldrich
Sigma-Aldrich

Thermo Fisher Scientific

Thermo Fisher Scientific
Thermo Fisher Scientific

Purification
Ni Seraphose 6 Fast Fow Resin 500 ml
GE Healthcare
SDS (500 g)
Thermo Fisher Scientific
Coomassie Blue G-250 (50 g)
Thermo Fisher Scientific
Imidazole 500 g
Thermo Fisher Scientific
Tris-Tricine 16.5% Gel Cartridge (pkg 2)
Bio Rad
Glycerol (1 L) 99.5
Promega
Sephadex G-25 resin (for desalting and buffer exchange) 1kg [$413.00/GE Healthcare
Total
UPLC System
Alliance
Waters Acquity UPLC BEH C18 Column (particle diameter 1.7m;Waters
column
length 100mm)
Sigma-Aldrich
Trifluoroacetic acid 1L (Reagent Plus)
Acetonitrile anhydrous 100 ml 98%
Sigma-Aldrich
Gravity Flow Columns (Econo-Pac- 50 Columns)
Bio-Rad
Total
Protein Concentration
Bradford Assay reagents
Protein Stability

Thermo Fisher Scientific

Value AUD
72.5
155
190
115
532.5

154.7
752.37
253.1
1160.17

7612
348.2
204.57
192
40
108
4130.00
12635

825
616
85.5
420
1947

221.82

ELISA kit 48-strip-well

mybiosource.com

753.8292

Labware
1.5 ml Microcentrifuge Tubes (pack of 500)
0.5 ml Microcentrifuge Tubes (pack of 1000)
Pipettes ($582/unit)
100-1000 l
10-100 l
0.5-10 l
Agar Dishes (Pack of 500)
Lab Coats
Disposable Gloves
Microplates (80 Plates/order)
Safety Glasses
Waste Bin
Total

Supplier
Sigma-Aldrich
Sigma-Aldrich
Eppendorf
Eppendorf
Eppendorf
Fisherbrand
The Coop ($24.95
Ansell
Eppendorf
ebay.com.au
seton.net.au

Value AUD
19.5
106
1164
1164
1164
205
199.6
17.17
303.6
5
62.65
4410.52