CHEMISTRY PROJECT ON COCONUT WATER STUDENT PARTICULARS:- > NAME:-Dipmallya Sen > CLASS:-XII > BOARD ROLL NO:- 6617658 > SESSION:-2014-2015 aVOLE DEELEELELELEEEEEEEbEEEELOLbIE TEACHER’S CERTIFICATE This is to certify that Dipmallya Sen of class XII/F (SCIENCE) of Hem Sheela Model School,Durgapur who is going to appear in AISCE -2015 has completed all his practical project in Chemistry during the session 2014-2015 with sincerity under my guidance. Therefore ,I certify that this project has been done according to the prescribed CBSE rules and regulation. External Examiner Internal Examinerrobb bbb ob bbbbbbbbbobbbbobbbou bla ty ls o ot, viet ACKNOWLEDGEMENT 1,Dipmallya Sen of class XII/F(SCIENCE) sincerely convey my heartiest thanks and sincere gratitude to my chemistry teachers Mr Pinaki Chatterjee and Renu Lakshmi madam for all the guidance and encouragement provided by them during the completion of my project . I would also like to thanks my parents who have extended. their helping hands towards me and have supported me and assisted me in doing this project.ebb bbELEE bib h bbb bh hhh bt hh tht tt bh CONTENTS 4@ AIM 4 INTRODUCTION # REQUIREMENTS 4 PH Tests «@ WET TEST FOR BASIC RADICALS 4@ WET TEST FOR ACID RADICALS 4 TEST FOR PRESENCE OF FOOD STUFF 4@ BIBLIOGRAPHYLb bb bb fo he flo fh oto bbb, bobbEbEnhbt bb | bb AIM Qualititive analysis of the food ingredients present in the coconut water. } yLEG6 l 66 uy FR A a Ly my, LD 7) al INTRODUCTION Constituents of coconut water Enerey kcal [1% Coconut water is the juice in the interior or Carbohydrates _|3.71g | 3% endosperm of young coconut. Its water is | Protein O72e [15% one of the nature’s most refreshing drinks, Total Fat 0.20g 1% ide for i tet, Se cra a consumed worldwide for its nutritious and Mee RRS health benefit properties. Folates 3 microg | 0.75% z f ‘ Te oaso rma OST The water is actually obtained by opening a Pantothenic acd | 0.043 mg tender , green healthy and undamaged | Pyridoxime 0.032 mg | coconut.Inside, it’s clear liquid is sweet and | Riboflavin 0.057 mg, | sterile and composed of unique chemicals entamnia) | such as sugars, vitamins, VitaminC Leese ee \ eanin k ou minerals,electrolytes,enzymes,amino: Vitamin E ona acids,cytokine and phyto-kine. Vitamin K ‘O meg cai Sa As for health benefits of coconut, the nates 250mg [5% | Possibilities are huge.tt is called “The tree Calcium amg | 2.4% of life” by the island people, because of the Copper 40meg | 4.5% fact that it produces immense food and cor 0.29mg_ |35% | other benefits to the people. | Magnesium 2me__| 6% Manganese 0.142 mg | 0% Coconut benefits in curing health ailments zing OrLOig [RL like malnutrition,nausea, kidney stone, Auxin Present = . i —_— jaundice,gonorrhea,tumor,influenza, sore Carotene O microg 3 i Cytokines | Present _ throat and various other ailments.It also Lutein- Omicrog |— helps in providing essential nutrition to zeaxanthin pregnant women and helps in improving = the bone density in kids.In modern medicine benefits of coconut are viewed as a universal medicine.|t helps in killing viruses of complex diseases like Hepatitis AIDS, measles,herpes, etc.Also various Leucoantocyanin | Presentob bGELELELEELELELELELEGEEELEEELEEGELbUG types of bacteria can be controlled by eating coconut such as throat infections,ulcers disease of gums,pneumonia and other types of bacteria related ailments.Researchers have suggested the possibility that consuming a rich source of cytokins, such as coconut water , may produce an anti-aging effect on the body, reducing rixk of developing degenerative and age related diseases. Health benefits of coconut water > Coconut water is a very refreshing drink to beat tropical summer thirst.The juice is packed with simple sugar, electrolytes and minerals to replenish hydration levels within the body. > Research studies suggest that cytokins(e.g. kinetin and trans-zeatin)in coconut water showed significant anti-ageing, anti-carcinogenic and anti-thrombotic effects. > Coconut water is composed of many naturally occuring bioactive enzymes such as acid phosphatase, catalase, dehydrogenase, diatase, peroxidase, RNA-polymerases etc. > Despite very light consistency, its water has much better composition of minerals like calcium, manganese, magnesium and zinc that some of the fruits like oranges contain > its water is also a very good source of B-complex vitamins such as riboflavin,niacin, thaimin,pyridoxime and foaltes.These vitamins are essential in the sense that the human body requires them from essential sources to replenish. > At home,store raw tender coconuts at room tempearture for about 5-10 days.Once opened, its water turns sour in taste and off-flavoured-due to intense enzymatic activity.Instead , store it inside the refrigerdtor if not used immediately.EELEELELELELELELELELELEEELEEEENVEGDOYE REQIREMENTS > Coconut water > FeSO, solution > Concentrated HSO, > AgNO; > Pb(CH3COO), > (NHa)2MoOq > Concentrated HNO3 > K,Cr,07 > Potassium pyroantimonite > Universal Indicator > NaOH solution > Ethylene Benedict’s solution > CuSO, > Disodium Hydrogen Phosphate(Na2HPO,) > NH,Cl > NH,OH > ((NH4)2COs)pH_TESTS coconut water is tested using universal indicator observed on pH paper Experiment Observation Inference pH of fermented A orange tinge is pH=5 coconut water is observed on pH tested using paper universal indicator pH of fermented A green tinge is pH=6 . PEELE ODOECEE EEE EEE LEE DOG OSES 7 evro EDLEEEELELELEEEELLELELEGELEEGLLGG WET TEST FOR BASIC RADICAL OBSERVATION EXPERIMENT | INFERENCE Confirmation | White ps is Na’ is confirmed, test for Na* | observed, 2NaCl+K,H,Sb,0, fons-Potassium pyro antimonite is added to ~Na.H.Sb,07| +2KCI coconut water. Confirmation of | Yellow ppt. is observed. | K*igns is confirmed. Hions- Freshly 3K* Na,[CO(NO),] Prepared cobalt nitrate solution is added to Smt —K,[CO(NO_),]+ of coconut water. 3Na* Mg” ions- Add NH,CI and NH,OH Solution to coconut water followed by NazPO, solution.Stir well | Confirmation of | White ppt. is observed. Mg ions are confirmed. Mg*+ NH,OH +HPO,7+5H,0— ‘ Mg(NH,)PO,.H,O 7 va_ WET TEST FOR ACID RADICALS AgNOs3 solution Confirmation of NO; ions- Mix freshly prepared FeSO, solution and add glacial acetic acid carefully along the wall of the test tube,so that it forms a separate layer. | EXPERIMENT | OBSERVATION | INFERENCE 7 | Confirmation | Mite vet readily | sence of CF ions are confimned of Cl ions. | lublein NHOH.| Ag*+ Cl—AgCl| Alcidify about 2m of the soda extract with AgCli + NH,—[Ag(NH3)2] concentrated HNO +Cr | and boi off CO, Add an Brown ring at the junction of the 2 layers | [Fe(H,0),]SO, + NO* — NaNO, + CH,;COOH — Na(CH;COO) + HNO,. 3HNO, — 2NO* + HNO, + H,0 ee +H,0/ PEEELEEEELELELELLELLGELLELEEELDbLLSGGLELEEDHbEELENG DD bdbdOOD HbbbLELEELEEES EXPERIMENT OBSERVATION | INFERENCE Chromy!l chloride test- Mix a small amount of powdered potassium dichromate.Take the mixture in a test tube and” add concentrated H,SO, seat the tube and pass the red vapours evolved into the gas detector containing NaOWibsolution. To the yellow solution thus obtained,add dilute CH,COOH ard lead acetate to it. A yellow ppt. is formed. [GM is confirmed. 4NaCI+K,CrO,+ |3H,SO, > Na,S0,+K,SO,+ 2Cr0,CI,+3H,0 Cr02CI2+4Na0H—> Na,Cr0, +H,0+2NaCl Na,Cr0,+(CH,COO) Pb—PbCr0,| +CH,COONa- fo |bb bbbbEbLEbEEbELELbDEbELDEDLEDLOEDG Phosphate test-Acidify coconut water with concentrated HNO sand boil off CO 2. Treat the solution with eccess ammonium molybdate solution. Warm the test in water bath and allow to stand. Oxalate test- Aacidify the coconut water with H2SO, and boil off COz .Then add a few drops of KMnO, Canary yellow ppt. indicates presence of phosphate ion. Purple colour of is KMnO géischarged Presence of phosphate is confirmed. K,P0,+3(NH,).MoO, —2(NH,);P0,+ K,MoO, K,MoO, +2HNO,—> H,Mo0,+KNO,+ H,Mo0,+Mo0O,+H,0 Presence of oxalate is confirmed. 2KMn0O,+ H,SO,— K,S0,+2MnSO,+ 10CO,+8H,0bbb bbbbLEEEEEEEEELEEGEGESEEEbLbDObLS DD TEST FOR PRESENCE OF FOOD STUFF EXPERIMENT OBSERVATION INFERENCE BENEDICT’S TEST- senedict’s No precipitate is observed. solution is added to 5m of coconut water and heated in water bath for 5 minutes. METHYLENE BLUE TEST-1-2 No decolourisation is drops of methylene blue is | 0bserved. Reducing sugar is absent, Reducing sugar is absent added to coconut water. neta cea ereeae CUSO4*N20H | 1 sive precipita is Proteins and fats are TEST-Copper sulphate | obtained: pee and NaOH is added to | coconut water Lea) yo e Te W beh wr| BIBLIOGRAPHY © www.wikipedia.com e NCERT CHEMISTRY book © Arihant & Comprehensive Chemistry ° www.nutritiondata.com ete e bebe bbe lef bbb bobddddd