Bacterial Infection of Wound

By

Dr. Humodi A. Saeed

Associate Prof. of Medical Microbiology
College of Medical Laboratory Science
Sudan University of Science and Technology
E mail address: Biotechsust@hotmail.com
Bacterial Infection of Wound
 The Wound

Definition
A wound is a breach (damage) in a normal tissue (May
be superficial or deep) resulting accidentally or of
.planned surgery
 The Wound

Types
1. Early acute wound
Long-term chronic wounds .2
 Bacterial Infection of Wound
• Wound contamination
• Wound colonization
• Wound Infection
 Bacterial Infection of Wound
• Wound contamination
• The presence of non-
replicating bacteria in
the wound.
 Bacterial Infection of Wound
Wound contamination
• All chronic wounds are contaminated by bacteria.
 Bacterial Infection of Wound

• Wound contamination
• contaminants come from the indigenous
microflora and/or the environment.
 Bacterial Infection Wound
• Wound colonization:
• The presence of replicating bacteria adherent to the
wound in the absence of injury to the host.
 Bacterial Infection of Wound

• Wound colonization:
Most of the following organisms are normal
skin flora.
1. S. epidermidis,
2. other coagulase –ve Staphylococci.
3. Corynebacterium sp.
4. Brevibacterium sp.
5. Proprionibacterium acnes
 Bacterial Infection of Wound

Wound Infection

The presence of replicating bacteria within a wound

that cause host injury.
 Bacterial Infection of Wound

Wound Infection
1. Staphylococcus aureus, .
2. Beta-hemolytic Streptococcus
(S. pyogenes, S. agalactiae)
3. E. coli
4. Proteus
5. Klebsiella
6. Anaerobes
7. Pseudomonas
8. Acinetobacter
 Bacterial Infection of Wound
Lab. Diagnosis
specimen collection.
1. Tissue biopsy
2. Needle aspirate
3. Wound swab
(Surface or Deep wound)
 Bacterial Infection of Wound
Lab. Diagnosis
• Suspected organism
Bite wounds
Eikenella corrodens, Pasteurella multocida, Pasteurella canis,
Staphylococcus aureus, group A Streptococcus, mixed anaerobe,
many gram-negative bacilli.
• Surgical wounds ­
Staphylococr:us aureus, coagulase-negative Staphylococcus, groups
A and B Streptococci, Clostridium perfringens, Corynebacterium
species many other bacteria.
• Traumatic wounds
Bacillus species, Staphylococcus aureus, group A
Strepiococcus, many gram­negative bacilli.
 Thank you

I would like to thank

Dr. Neal R. Chamberlain