Plasmid extraction kit

Buffer P1 (cell suspension buffer)

50mM Tris-HCl pH 8.0 (25℃)
10mM EDTA
50ug/ml of RNase A

Buffer P2 (lysis buffer)

0.2M NaCl and 1% SDS

Buffer N3 (neutralization and binding buffer)

4M guanidine hydrochloride (GuHCl)
0.5M potassium acetate pH 4.2

Buffer PB (wash buffer)

5M guanidine hydrochloride (GuHCl)
20mM Tris-HCl pH 6.6 (25℃)
38% ethanol

Buffer PE (wash buffer)

20mM NaCl
2mM Tris-HCl pH 7.5 (25℃)
80% ethanol

Buffer EB (elution buffer)

10mM Tris-HCl pH 8.5 (25℃)

Gel extraction kit (DNA fragment recovery from agarose gel)

Buffer QG (neutralization and binding buffer)
5.5M guanidine thiocyanate (GuSCN)
20mM Tris-HCl pH 6.6 (25℃)

Buffer PE (wash buffer)

20mM NaCl
2mM Tris-HCl pH 7.5 (25℃)
80% ethanol

Buffer EB (elution buffer)

10mM Tris-HCl pH 8.5 (25℃)

PCR purification kit (Direct purification of PCR products without

electrophoresis)
Buffer PB (DNA binding buffer)
5.5 M guanidine hydrochloride (GuHCl)
20mM Tris-HCl pH 6.6 (25℃)

Buffer PE (wash buffer)

20mM NaCl
2mM Tris-HCl pH 7.5 (25℃)
80% ethanol

Buffer EB (elution buffer)

10mM Tris-HCl pH 8.5 (25℃)