Lane 1 contained an undigested pUC19 plasmid, lanes 2-4 contained the same plasmid cut with different restriction enzymes Bam HI, Eco RI and Pst I respectively, showing fragments of the expected sizes. Lane M contained a 1 kb ladder molecular weight marker run on the same 1% agarose gel for size comparison.
Lane 1 contained an undigested pUC19 plasmid, lanes 2-4 contained the same plasmid cut with different restriction enzymes Bam HI, Eco RI and Pst I respectively, showing fragments of the expected sizes. Lane M contained a 1 kb ladder molecular weight marker run on the same 1% agarose gel for size comparison.
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Lane 1 contained an undigested pUC19 plasmid, lanes 2-4 contained the same plasmid cut with different restriction enzymes Bam HI, Eco RI and Pst I respectively, showing fragments of the expected sizes. Lane M contained a 1 kb ladder molecular weight marker run on the same 1% agarose gel for size comparison.
Copyright:
Attribution Non-Commercial (BY-NC)
Available Formats
Download as DOCX, PDF, TXT or read online from Scribd
purified from JM109 using the GenElute Plasmid Miniprep Kit. Lane 1 was an undigested plasmid, lanes 2-4 were cut with Bam HI, Eco RI and Pst I respectively. Lane M was 1 kb ladder (11 fragments from 0.5 to 10 kb). Electrophoresis was on a 1% agarose gel.