##BLOCKS= 5

Note:
To use this Protocol:
1. Delete unwanted Sections.
2. Modify the Instrument Settings based on your specific reader by pressing [Set
up].
3. Click the [Template] button and assign any default or new groups as necessary
. You can print the assigned template to help prepare your microplate.
4. Select the desired Reduction and Display (Raw vs. Reduced) parameters.
5. Save this protocol with a different name in the same folder as the SoftMax Pr
o application. This modified protocol can now be accessed through the Assays men
u. A copy of the original protocol can be found in the original location.
~End
Plate: Plate#1 1.3 PlateFormat Endpoint Absorbance Raw
FALSE 1 1 450 1
12 96 1 8 None
Temperature(¡C) 1 2 3 4 5 6 7
8 9 10 11 12
25.10 0.0869 0.0717 0.086 0.0755 0.0729 0.0684 0.6077 0.5806
0.641 0.4217 0.4422 0.5127
0.0619 0.0864 0.0792 0.0591 0.0577 0.0845 0.3964 0.3983
0.4226 0.2651 0.2645 0.2678
0.0631 0.0569 0.0567 0.0522 0.0482 0.0514 0.3437 0.3257
0.3518 0.2218 0.2233 0.226
0.0517 0.0566 0.0567 0.0511 0.0553 0.051 0.1423 0.1705
0.1595 0.1383 0.1378 0.1454
0.052 0.0529 0.0515 0.0569 0.0519 0.0485 0.1192 0.111
0.1378 0.1206 0.1327 0.1353
0.0523 0.0518 0.3195 0.0504 0.0503 0.0478 1.0724 0.0786
0.0769 0.077 0.0768 0.0726
0.0494 0.0497 0.049 0.0473 0.0471 0.0475 0.0608 0.0651
0.0671 0.0597 0.0599 0.0615
0.0687 0.049 0.0522 0.0492 0.0488 0.0512 0.0601 0.0556
0.0676 0.0583 0.0634 0.0529
1 2 3 4 5 6 7 8
9 10 11 12
0.0869 0.0717 0.086 0.0755 0.0729 0.0684 0.6077 0.5806
0.641 0.4217 0.4422 0.5127
0.0619 0.0864 0.0792 0.0591 0.0577 0.0845 0.3964 0.3983
0.4226 0.2651 0.2645 0.2678
0.0631 0.0569 0.0567 0.0522 0.0482 0.0514 0.3437 0.3257
0.3518 0.2218 0.2233 0.226
0.0517 0.0566 0.0567 0.0511 0.0553 0.051 0.1423 0.1705
0.1595 0.1383 0.1378 0.1454
0.052 0.0529 0.0515 0.0569 0.0519 0.0485 0.1192 0.111
0.1378 0.1206 0.1327 0.1353
0.0523 0.0518 0.3195 0.0504 0.0503 0.0478 1.0724 0.0786
0.0769 0.077 0.0768 0.0726
0.0494 0.0497 0.049 0.0473 0.0471 0.0475 0.0608 0.0651
0.0671 0.0597 0.0599 0.0615
0.0687 0.049 0.0522 0.0492 0.0488 0.0512 0.0601 0.0556
0.0676 0.0583 0.0634 0.0529
~End
Group: Standards 1
Sample Concentration Back Calc Conc Wells OD Values Mean OD Value
Std.Dev. CV%
~End
Group: Unknowns 1
Sample Wells OD Values Concentration Mean Conc. Std.Dev.
CV% Dilution Adj. Conc.
~End
Group: Unknowns(no diln) 1
Sample Wells OD Values Concentration Mean Conc. Std.Dev.
CV%
R - Outside standard range
~End
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