Inhibition of MexXY-OrpM efflux system reduce virulence

from intrinsic multidrug resistance in Pseudomonas aeruginosa

Introduction
Pseudomonas aeruginosa is an opportunistic human pathogen characterized by an
innate resistance to multiple antimicrobial agents. A major contribution to this intrinsic
multidrug resistance is provided by a number of broadly-specific multidrug efflux systems,
including MexAB-OprM and MexXY-OprM.( Multidrug Efflux Pumps)

Pseudomonas aeruginosa is primarily encountered as a nosocomial pathogen in adult clinical

medicine (National Nosocomial Infections) . Outside the hospital, it is commonly found in
soil, water, and plants and can on occasion, be associated with a colonization of otherwise
healthy humans and animals.( Pier GB, Reuben R. Pseudomonas aeruginosa) Within the
hospitals, it can colonize moist surfaces including inanimate environment like water in sinks
and drains. Hospital equipment that comes in contact with water such as ventilators can be the
source of P. aeruginosa. Prior colonization with P. aeruginosa is frequently associated with
later invasive infection.

Materials and Methods

Bacterial strains and growth conditions
The bacterial strains and plasmids used in this study were listed in
Table 1. Luria-Bertani (LB), Brian Heart Infusion (BHI), and
Pseudomonas Isolate Agar (PIA) were used routinely. Defatted milk
solid medium which is LB media supplemented with 2% defatted
milk was used for measuring proteolytic activity and blood agar plates
were used for measuring hemolytic activity. M8 medium (Sambrook
References
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2. Pier GB, Reuben R. Pseudomonas aeruginosa. In: Mandell GL, Bennett GE, Dolin R,
editors. Mandell, Douglas and Bennett’s Principle and practice of infectious diseases. 6 th ed.
Philadelphia: Elsevier Churchill Livingstone; 2005: 2587-615.