Cu Staining of PAGE gels Normal SDS-PAGE gels can be stained in 5 min with 300mM CuCl2. 1.

Simply remove the gel, rinse ~10s in water 2. Place 5 min in CuCl2 3.The gel will be negatively stained, with protein bands appearing clear, and background opaque white. 4. To visualize, put it on a flatbed scanner in a darkened room. Ref. Lee et al, Anal. Biochem. 1987. Results in 5 min with copper! Gels are stable in H2O for months,