Scribd Logo
Upload

Welcome to Scribd!

  • Making E

    Uploaded by

    Dhaval Shah
    11 views1 page
    The document provides instructions for making and using an E. coli glycerol stock. To make the stock, E. coli is grown to mid-log phase, 800 ul of the culture is added to a sterile tube along with 200 ul of autoclaved glycerol, and the mixture is vortexed and stored at -80°C. To use the stock, the surface of the frozen glycerol stock is touched with a sterile pipette tip which is then used to inoculate 5 ml of LB media.

    Original Description:

    Copyright

    © Attribution Non-Commercial (BY-NC)

    Available Formats

    DOCX, PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Report this Document
    The document provides instructions for making and using an E. coli glycerol stock. To make the stock, E. coli is grown to mid-log phase, 800 ul of the culture is added to a sterile tube along with 200 ul of autoclaved glycerol, and the mixture is vortexed and stored at -80°C. To use the stock, the surface of the frozen glycerol stock is touched with a sterile pipette tip which is then used to inoculate 5 ml of LB media.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOCX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    11 views1 page

    Making E

    Uploaded by

    Dhaval Shah
    The document provides instructions for making and using an E. coli glycerol stock. To make the stock, E. coli is grown to mid-log phase, 800 ul of the culture is added to a sterile tube along with 200 ul of autoclaved glycerol, and the mixture is vortexed and stored at -80°C. To use the stock, the surface of the frozen glycerol stock is touched with a sterile pipette tip which is then used to inoculate 5 ml of LB media.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOCX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 1

    Making E-coli glycerol stock: Material needed: Growing E-coli (mid log phase) 1- 80% glycerol stock (in

    water, autoclaved) To make the E-coli stock: 1- Inoculate 5 ml LB media using a single colony. Allow to grow to mid log phase. Do not allow to saturate. 2- In sterile tube (o-ring tubes), add 800 ul of growing E-coli. 3- Add 200 ul of 80% autoclaved glycerol. 4- Mix briefly by vortexing and store at -80 C

    Growing cells from glycerol stock: With sterile pipet tip, touch the surface of the glycerol stock (DO NOT THAW), and throw the tip in 5 ml LB media.

    ) A culture of anaerobic bacteria made by piercing a solid agar medium in a test tube with an inoculating needle covered with the bacterial inoculum. Read more: http://www.answers.com/topic/stab-culture#ixzz1VSv5ZGsA

    You might also like