STRUKTUR DAN FUNGSI ASID NUKLEIK (DNA / RNA) is the helical structure of DNA. Each complete helical turn is 34A (3. Nm) long, so there are 10 base pairs per helix.
STRUKTUR DAN FUNGSI ASID NUKLEIK (DNA / RNA) is the helical structure of DNA. Each complete helical turn is 34A (3. Nm) long, so there are 10 base pairs per helix.
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STRUKTUR DAN FUNGSI ASID NUKLEIK (DNA / RNA) is the helical structure of DNA. Each complete helical turn is 34A (3. Nm) long, so there are 10 base pairs per helix.
Copyright:
Attribution Non-Commercial (BY-NC)
Available Formats
Download as PPT, PDF, TXT or read online from Scribd
- Composition - Nitrogenous Bases (purine vs pyrimidine) - Nucleoside vs nucleotide - Nucleoside mono-, di- and triphosphate - Polynucleotide B. Structure of DNA/RNA
DNA/RNA Composition Studies (bases):
1. Erwin Chargaff (1950)
- A=T - CΞG - A + G = C + T ↔ (A + G)/(C + T) = 1.0 - but, (A + T)/(C + G) ≠ 1.0 1. X-ray Diffraction
– William Ashbury (1947) – periodicity of 3.4 Å and the
the bases are stacked on top of each other
– Rosalind Franklin and Maurice Wilkins (1950 - 1953)
– confirmed periodicity and proposed the helical
structure of DNA
– Linus Pauling visualized DNA as a triple helix
DNA – Watson-Crick (1953) - Two polynucleotide strands are coiled around one axis forming a double helic - Both strands are in anti-parallel orientation - Sugar-phosphate backbone located on the outside - The bonded bases of both strands are located within the helix and are stacked 3.4Å (0.34 nm) apart, and are perpendicular to the axis - Nitrogenous bases of each strands are bonded pair-wise to bases of the other strands via hydrogen bonding (A=T dan CΞG) - Each complete helical turn is 34Å (3.4 nm) long, so there are 10 base pairs per helical turn - Presence of major and minor grooves - The helix diameter is 20Å (2.0 nm) - Right-handed helical turn - Type B DNA Other Forms of DNA:
DNA-A ─ 11 base pairs/helical turns, dameter 23Å
DNA-B ─ 10 base pairs/helical turns, diameter 20Å
DNA-C ─ 9.3 base pairs/helical turns, diameter 19Å
DNA-D ─ base pairs/helical turns, no CΞG pairs
DNA-E ─ 7.5 base pairs/helical turns, no CΞG pairs
DNA-Z ─ base pairs/helical turns, diameter 18Å, only CΞG pairs,