Laboratory Diagnosis of Viral Infection

TBL MCQs

1- Direct viral detection is used to detect all of the following

except:
a)virus particles

b)viral antigens
c)viral nucleic acid

d)Antibodies to the virus

2- All the following techniques are useful for diagnosis of viral infection via direct detection of the components of the virus except:
a) Electron Microscopy b)Indirect immunofluorescence staining c)Probing d)ELISA for Antibody detection for diagnosis of the viral infection

3- The tissue culture cell line which is derived from somatic cells and can not be subcultured is:
a)Primary cell lines b)Semicontinuous cell lines

c)Continuous cell lines

d)Chick embryo

4-The most useful diagnostic approach for diagnosis of a novel viral infection is:
a)PCR b)Isolation on tissue culture cell lines

c)Immunoflurescent staining
d)Probing

5- The technique useful for detection of antibody response to multiple antigenic component of the virus is:
a)PCR

b)Probes
c)Immunoblot d)ELISA

6. A serological diagnosis of a primary viral infection may be made:

a)Detection of viral-specific IgA b)Detection of viral-specific IgD c)Detection of viral-specific IgE d)Detection of viral-specific IgM

7. Regarding cell culture which of the following is correct?

a)Viruses can only be cultured using cell

lines. b)The presence of cytopathic effect is the only way to detect a virus. c)Antigen detection is the only way to detect a virus. d)Viruses may also be cultured in eggs and animals.

8 one of The following is an example of viral genome detection (molecular methods).

a)ELISA b)Indirect immunoflourescence

c)RIBA (Recombinant immunoblot

assay) d)Polymerase chain reaction (PCR)

9. All is true regarding interference except :

a) Used to detect viruses not producing

cytopathic effect. b) Cell appear normal but no longer susceptible to super infection. c) No cytopathic effect will be produced. d) CPE will be produced.

10. Heamagglutination is defined as:

a) appearance of heamagglutinin in cell

culture fluid. b) attachment of erythrocytes to viral infected cells. c)Cellular changes induced by viruses in TCC. d)Detection of viral nucleic acid by molecular methods.

Model Answer:

1(d) 2(d) 3(a) 4(b) 5(c) 6(d) 7(d) 8(d) 9(d) 10(a)