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    The document summarizes research on the complete mitochondrial DNA sequence of the Lake Biwa wild strain of common carp in Japan. Key findings include: 1) Analysis of the complete mtDNA sequence found further evidence that the Lake Biwa wild strain is distinct from Eurasian strains of common carp. 2) Phylogenetic analysis of multiple gene regions from various carp strains across Asia recovered the distinction between the Lake Biwa wild strain and Eurasian strains. 3) Relationships among Eurasian strains, including Japanese ornamental koi, could not be reliably reconstructed due to low nucleotide divergence across the mitochondrial genome.

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    The document summarizes research on the complete mitochondrial DNA sequence of the Lake Biwa wild strain of common carp in Japan. Key findings include: 1) Analysis of the complete mtDNA sequence found further evidence that the Lake Biwa wild strain is distinct from Eurasian strains of common carp. 2) Phylogenetic analysis of multiple gene regions from various carp strains across Asia recovered the distinction between the Lake Biwa wild strain and Eurasian strains. 3) Relationships among Eurasian strains, including Japanese ornamental koi, could not be reliably reconstructed due to low nucleotide divergence across the mitochondrial genome.

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    13 views1 page

    Aquacultur 1

    Uploaded by

    Rinku Arora
    The document summarizes research on the complete mitochondrial DNA sequence of the Lake Biwa wild strain of common carp in Japan. Key findings include: 1) Analysis of the complete mtDNA sequence found further evidence that the Lake Biwa wild strain is distinct from Eurasian strains of common carp. 2) Phylogenetic analysis of multiple gene regions from various carp strains across Asia recovered the distinction between the Lake Biwa wild strain and Eurasian strains. 3) Relationships among Eurasian strains, including Japanese ornamental koi, could not be reliably reconstructed due to low nucleotide divergence across the mitochondrial genome.

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    Aquaculture Volume 257, Issues 1-4, 30 June 2006, Pages 68-77 Complete mitochondrial DNA sequence of the Lake

    Biwa wild strain of common carp (Cyprinus carpio L.): further evidence for an ancient origin Kohji Mabuchia, Mutsumi Nishidaa
    a ,

    , Masaki Miyab, Hiroshi Senouc, Toshiyuki Suzukid and

    Ocean Research Institute, The University of Tokyo, 1-15-1 Minamidai, Nakano-ku, Tokyo 164-8639, Japan
    b

    Department of Zoology, Natural History Museum and Institute, Chiba, 955-2 Aoba-cho, Chuo-ku, Chiba 260-8682, Japan
    c

    Kanagawa Prefectural Museum of Natural History, 499 Iryuda, Odawara, Kanagawa 250-0031, Japan
    d

    Amagasaki kita Senior High School, 5-40-1 Tsukaguchimachi, Amagasaki, Hyogo 6610002, Japan

    Abstract
    The complete mitochondrial DNA (mtDNA) sequence of Lake Biwa wild common carp (LBW) was determined using a PCR-based method, and the phylogenetic status of the LBW strain within common carp was re-examined using a number of already-published mtDNA sequences of multiple gene regions from various strains, which enabled a geographically more comprehensive analysis including additional strains from South-East Asia (Vietnam and Indonesia). The following three datasets, dataset A [partial D-loop region sequences (ca. 760 bp) from 44 strains], B [ATPase6 and 8 gene sequences (826 bp) from 39 strains] and C (COII gene sequences (690 bp) from 9 strains), were constructed from different studies, and phylogenetic analyses conducted using a goldfish (Carassius auratus auratus) as an outgroup. In all the analyses, a principal phylogenetic dichotomy between the LBW and Eurasian strains, which was recently revealed based on cyt b gene and D-loop region sequences, was recovered. Phylogenetic relationships among Eurasian (including Japanese ornamental koi) strains were, however, not reconstructed reliably. Low level nucleotide divergence throughout the mitochondrial genome, which was revealed by the whole mtDNA comparison between the LBW and a putative Taiwanese strain, indicated that no single coding/non-coding region could provide sufficient information for resolving phylogeny among the closely-related Eurasian strains of the species.

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