Species Diversity 26: 145–151

Published online 17 June 2021

DOI: 10.12782/specdiv.26.145

A New Species of the Genus Eucorydia (Blattodea:

Corydiidae) from the Miyako-jima Island in Southwest Japan
Shizuma Yanagisawa1,7, Shimpei F. Hiruta2,
Yositaka Sakamaki3, and Satoshi Shimano4,5,6
1 Ryuyo Insect Nature Observation Park, 320-1 Oonakaze, Iwata, Shizuoka 438-0214, Japan
E-mail: yokoyama070821@gmail.com 
2 Center for Molecular Biodiversity Research, National Museum of Nature and Science, 4-1-1 Amakubo, Tsukuba, Ibaraki 305-0005, Japan
3 Entomological Laboratory, Faculty of Agriculture, Kagoshima University, Korimoto, Kagoshima 890-0065, Japan
4 Science Research Center, Hosei University, 2-17-1 Fujimi, Chiyoda-ku, Tokyo 102-8160, Japan
5 Visiting Researcher, International Center for Island Studies, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-8580, Japan
6 Visiting Researcher, Graduate School of Science, Tokyo Metropolitan University, 1-1 Minami-osawa Hachioji-shi, Tokyo 192-0397, Japan
7 Corresponding author

(Received 2 December 2020; Accepted 10 March 2021)

http://zoobank.org/E6F9409F-E720-437E-AA08-3D5E9542A49F

A new species from the cockroach genus Eucorydia Hebard, 1929 from Miyako-jima Island of the Nansei Islands in
Southwest Japan was compared to six closely related congeners; E. yasumatsui Asahina, 1971; E. donanensis Yanagisawa,
Sakamaki, and Shimano, 2020; E. tokaraensis Yanagisawa, Sakamaki, and Shimano, 2020; E. dasytoides (Walker, 1868); E.
guilinensis Qiu, Che, and Wang, 2017; and E. pilosa Qiu, Che, and Wang, 2017. The new species Eucorydia miyakoensis
Yanagisawa, Sakamaki, and Shimano, sp. nov. from Miyako-jima Island was characterized by a small overall male body
length of 12.5–13.0 mm and tegmina with an uninterrupted orange transversal band in the middle, and a pair of orange pu-
bescent patches at the base. Eucorydia yasumatsui, E. donanensis, E. tokaraensis, the zonata population of E. dasytoides, and
E. miyakoensis were divided into five lineages in a maximum likelihood tree generated from a dataset concatenated from
five molecular markers (two nuclear: 28SrRNA and histone H3, and three mitochondrial: COII, 12SrRNA, and 16SrRNA).
We recognized E. miyakoensis as a distinct species, which was also supported by the pairwise genetic distances (3.4%–6.7%,
K2P) of the COI sequences to the other Japanese Eucorydia species.
Key Words: genetic distances, multiple gene loci, genitalia, taxonomy.

with that of the E. miyakoensis sp. nov. from Miyako-jima Is-

Introduction
The cockroach genus Eucorydia Hebard, 1929 has a metal-
lic greenish blue pronotum and tegmina in adults and some
orange markings on the tegmina and/or abdomen of most
species. In Japan, E. yasumatsui Asahina, 1971 was described
from Iriomote-jima Island and Ishigaki-jima Island (Ashina
1971). Yanagisawa et al. (2020) described two additional spe-
cies, E. donanensis Yanagisawa, Sakamaki, and Shimano, 2020
from Yonaguni-jima Island and E. tokaraensis Yanagisawa,
Sakamaki, and Shimano, 2020 from Uji-Ie-jima Island, Ama-
mi-Oshima Island, Tokuno-shima Island, and Akuseki-jima
Island. Recently, we found another species of Eucorydia from
Miyako-jima Island in the collection of the Osaka Museum
of Natural History, which is thought to be an undescribed
species not previously included in the three known Japanese
species. To an undescribed species, we gave a new scientific
name, E. miyakoensis sp. nov. We succeeded in collecting ad-
ditional specimens from the same Island. The purpose of this
study was to clarify the identity of Eucorydia species from
Miyako-jima Island. We compared the external morphol-
ogy of four species from Japan and Taiwan [E. tokaraensis, E.
yasumatsui, E. donanensis, and E. dasytoides (Walker, 1868)]
land. To confirm the morphological studies, we inferred the
phylogenetic relationships between the species from Miyako-
jima Island and the other four species from Japan and Taiwan
using the DNA sequences from six loci.
Materials and Methods
A total of twelve specimens were examined in this study.
Nine specimens were collected from Gusukube, Miyako-
jima Island and three were cultured individuals originating
from Gusukube. All of E. miyakoensis sp. nov. specimens
examined in this study were identified using the unique al-
pha-numerals eM-001–012 and eMo-001–010, with “eMo”
referring to the oothecae. Adults and oothecae were ob-
tained by indoor rearing of the field-collected specimens
under natural daylight at 22°C–27°C and 50%–70% humid-
ity on a diet of “mouse food” (Rodents Diets MF; Oriental
Yeast Co., Ltd.). The morphological terminology used in
this paper mainly follows Qiu et al. (2017) and Yanagisawa
et al. (2020). Male genital segments were prepared for dis-
section by maceration in 10% NaOH to remove the protein
and muscles. They were then placed in 75% ethanol and

 © 2021 The Japanese Society of Systematic Zoology

146 Shizuma Yanagisawa et al.

observed under a stereomicroscope (ST-LED, Kenis) and
drawings were made based on these observations. Pho-
tographs were taken using a Nikon D5300 camera with a
Nikon AF-S VR Micro-Nikkor 105 mm f/2.8G IF-ED lens.
The type series for E. miyakoensis sp. nov. was deposited
into the Dictyoptera collection (NSMT-I-Dct) of the National
Museum of Nature and Science, Tsukuba (NMNS; previous
name, the National Science Museum, Tokyo: NSMT).
DNA extraction. Each specimen used for DNA analy-
sis was dissected under the stereomicroscope and their ap-
pendages were used for DNA extraction. The total genomic
DNA was extracted using a DNeasy Blood and Tissue Kit
(Qiagen), with modifications per Johnson et al. (2004). The
samples were incubated for at least 48 h to lyse the tissue.
The exoskeletons were retrieved from the appendages be-
fore each lysed mixture was pipetted into the spin column,
and preserved with the rest of the body in 100% ethanol as
voucher specimens.
PCR amplification and sequencing. The partial se-
quences of six genes were amplified (two nuclear: 28S rRNA
and histone H3, and four mitochondrial: COI, COII, 12S
rRNA, and 16S rRNA). Amplification of each target gene
was conducted using the primers listed in Table 1. The
PCR reactions were performed in a TaKaRa PCR Ther-
mal Cycler Dice Touch (TaKaRa) in 10 µL volumes, which
each contained 1 µL of the template solution, 2 mM MgCl2,
2.5 mM dNTP, 10 pmol of each primer, and 0.25 U Ex Taq
polymerase (TaKaRa) in 1× buffer provided by the manu-
facturer. The amplification conditions were 95°C for 2 min;
35 cycles at 98°C for 10 s; 50°C for 30 s; 72°C for 1 min (for
histone H3, COI, COII, 12S rRNA, and 16S rRNA) or 2 min
(for 28S rRNA); and 72°C for 7 min. The amplified prod-
ucts were purified using ExoSAP-IT Express PCR Cleanup
Reagents (Thermo Fisher Scientific, Waltham, MA, USA).
The nucleotide sequences were determined using direct se-
quencing via a BigDye Terminator Cycle Sequencing Kit
ver. 3.1 (Thermo Fisher Scientific) with an ABI 3500xL Ge-
netic Analyzer (Thermo Fisher Scientific). The amplifica-
tion primers and internal primers listed in Table 1 were also
used for sequencing. The determined sequences were depos-
ited in DDBJ (https://www.ddbj.nig.ac.jp/) under accession
numbers LC565385–LC565406.
Genetic analysis. To elucidate the phylogenetic relation-
ships of the new species from Miyako-jima Island and the
previously described Eucorydia species, maximum likeli-
hood (ML) and Bayesian (BI) phylogenetic analyses were
performed based on a dataset generated by concatenating
five molecular markers (28S, H3, COII, 12S, and 16S) by
obtaining sequences from two populations of E. dasytoides
and two species from Ergaula Walker, 1868, from GenBank,
which were regarded as outgroup taxa. For the phyloge-
netic reconstruction, COI sequences were excluded from
the dataset since those of the outgroup taxa were unavail-
able at GenBank. The nucleotide sequences were assembled
and edited using MEGA ver. 7 (Kumar et al. 2016). Concat-
enated phylogenetic trees were constructed based on ML
framework in IQ-TREE (Nguyen et al. 2015) and BI analyses
with MrBayes ver. 3.2.6 (Huelsenbeck and Ronquist 2001;
Ronquist and Huelsenbeck 2003). PartitionFinder ver. 2.1.1
(Lanfear et al. 2017) was used to determine the best parti-
tioning scheme and substitution model for IQ-TREE and
MrBayes using linked branch lengths and a greedy search
algorithm (Lanfear et al. 2012). For ML and BI analyses,
the corrected Akaike information criterion (AICc) and the
Bayesian information criterion (BIC) were used, respective-
ly. The optimal partitioning scheme and evolutionary mod-
els consisted of five gene data sets for both analyses (Table
2). Bootstrap analyses (Felsenstein 1985) of 1000 pseudo-
replicates were performed for ML tree. For BI analyses, the
Markov-chain Monte-Carlo process used random starting
trees and involved two runs of four chains each (three hot
and one cold) for 20 million generations. Trees were sampled
every 100th generation; the first 25% of trees were discarded
as burn-in. Convergence was inferred when the standard de-

Table  1.  List of primers used in this study.

Genes Primer name Sequence (5′–3′) Source used for PCR

28S rRNA 28S-01 GAC TAC CCC CTG AAT TTA AGC AT Kim et al. (2000) ○
28SR-01 GAC TCC TTG GTC CGT GTT TCA AG Kim et al. (2000)
28Sf TGG GAC CCG AAA GAT GGT G Luan et al. (2005)
28S-Euc11F ACG GAC CAA GGA GTC TAA CWT Yanagisawa et al. (2020)
28S-Euc16R TAA AGT TTG AGA ATA GGT TGA GGT C Yanagisawa et al. (2020)
28Sr ACA CAC TCC TTA GCG GA Luan et al. (2005)
28S_2KF TTG GAA TCC GCT AAG GAG TG Hiruta et al. (2016)
28S_3KR CCA ATC CTT TTC CCG AAG TT Hiruta et al. (2016) ○
H3 H3 AF ATG GCT CGT ACC AAG CAG ACV GC Inward et al. (2007) ○
H3 AR ATA TCC TTR GGC ATR ATR GTG AC Inward et al. (2007) ○
COI LCO1490 GGT CAA CAA ATC ATA AAG ATA TTG G Folmer et al. (1994) ○
HCO2198 TAA ACT TCA GGG TGA CCA AAA AAT CA ○
COII COII-F AGA GCW TCA CCT ATT ATA GAA C Park et al. (2004) ○
COII-R GTA RWA CRT CTG CTG CTG TTA C ○
12S rRNA 12S forward ATC TAT GTT ACG ACT TAT Inward et al. (2007) ○
12S reverse AAA CTA GGA TTA GAT ACC C Kambhampati (1995) ○
16S rRNA 16S Forward CGC CTG TTT AAC AAA AAC AT Simon et al. (1994) ○
16S Reverse TTT AAT CCA ACA TCG AGG Cognato and Vogler (2001) ○
 A new species of Eucorydia from the Miyako-jima Island
Table  2.  Substitution models used in this study as determined by
Partition Finder 2.1.1 (Lanfear et al. 2017)
Infomation Partition Character substitution
Criterion scheme set models
AICc 4 12S, 16S GTR+I
COII GTR+I
28S TRN+G
H3 SYM+G
BIC 4 12S, 16S GTR+I
COII HKY+I
28S HKY
H3 K80+G
viation of split frequencies became <0.01, and the potential
scale reduction factors were ~1.0. In addition, Tracer ver. 1.7
(Rambaut et al. 2018) was used to ensure that parameter val-
ues have an effective sampling size value of >200. Analysis
of Kimura-2-parameter (K2P) genetic distance between COI
sequences of Eucorydia was performed using MEGA7.
Results
Taxonomy
Eucorydia miyakoensis Yanagisawa, Sakamaki, and
Shimano, sp. nov.
[New Japanese name: Benieri-rurigokiburi]
(Figs 1A–P, 2)
Material examined. Holotype: male (NMNS, NSMT-I-
Dct-542), Gusukube, Miyako-jima Island, Okinawa, Japan,
12 November 2019, S. Yanagisawa leg. (eM-001). Paratypes:
2 males and 5 females (NMNS, NSMT-I-Dct-543–549),
Gusuku­be, Miyako-jima Island, Okinawa, Japan, 12 Novem-
ber 2019, S. Yanagisawa leg. (eM-002–eM-008).
Differential diagnosis. This new species resembles E.
guilinensis Qiu, Che, and Wang, 2017, E. dasytoides, E. yasu-
matsui, E. tokaraensis, E. donanensis, and E. pilosa Qiu, Che,
and Wang, 2017. However, it can be easily distinguished by
an uninterrupted transverse orange band in the middle of
the tegmina, L7 of male genitalia round the basal half, R2
round, and a pair of orange pubescent blotches at the base
of the tegmina in both males and females (Fig. 1A–D, F–H, J,
K). Eucorydia miyakoensis sp. nov. can be distinguished from
E. guilinensis and E. pilosa through characteristics of their
genitalia. The L7 of male genitalia of E. guilinensis is strongly
protruded in the anterior and curved toward left posterior
(Qiu et al. 2017), whereas that of E. miyakoensis sp. nov. is
round in the basal half (Fig. 1F, H). In addition, E. guilinensis
resembles E. miyakoensis sp. nov. in that it has pubescence at
the base of the tegmina; however, the pubescence of E. guili-
nensis is yellowish white, whereas that of E. miyakoensis sp.
nov. is orange (Fig. 1J, K). Furthermore, the overall length of
the male E. guilinensis is more than 13 mm (13.8–14.2 mm;
Qiu et al. 2017), whereas that of the male E. miyakoensis sp.
nov. is less than 13 mm (overall length of 12.5–13.0 mm; Fig.
147
1A–D). The R2 of male genitalia of E. pilosa is elongated and
slightly rhomboid (Qiu et al. 2017), whereas that of E. miya-
koensis sp. nov. is round (Fig. 1G). Eucorydia dasytoides and
E. pilosa are distinguishable from the new species in that
their males have an overall length >18 mm (Qiu et al. 2017),
whereas overall length of the male E. miyakoensis sp. nov. is
<13 mm. Both the size and shape of genitalia are similar to
those of the three Japanese species, namely E. yasumatsui,
E. tokaraensis, and E. donanensis, but it can be distinguished
from them by orange pubescence at the base of the tegmina.
Description. Male (n=3): Body length 11.5 mm; overall
length 12.5–13.0 mm; pronotum length 3.4–3.5 mm, width
5.6–5.9 mm; tegmen length 10.0–10.8 mm (Fig. 1A, B).
Head shiny, black. Antenna black, consisting of 36–38
segments with 6–7 whitish subapical segments. Numbers
of whitish segments sometimes different between the left
and right sides. Pronotum metallic blue to metallic blu-
ish green. Tegmina metallic blue to metallic bluish green,
similar to pronotum color with a pair of orange pubescent
blotches at the base near scutellum. Distal half with distinct,
uninterrupted orange band. Hindwings hyaline, pale brown,
becoming darker toward the apex with an orange blotch at
middle of costa. Sc single; RA+RP with 5–8 branches; M
single, but with one cell and some crossveins in middle part;
CuA with 8 branches; CuP single; and AA+AP with 11–14
branches (Fig. 1L). Legs shiny, black.
Dorsum dark purple with yellowish area occupied from
caudal half of 2nd segment to 5th segment; 6th segment
of tergite dark purple in middle and yellow on lateral sides
(Fig. 1M). Ventrum black, with yellowish area occupied
from 2nd to 6th segments. Supra-anal plate black, widely bi-
lobed; cercus consisting of 8 segments (Fig. 1E); subgenital
plate black, rounded, with styli.
Genitalia (Fig. 1F–I): Left phallomere: L3 slender and
curved, gradually narrowing toward the apex with a distinct
hook (Fig. 1I); L7 round in the basal half, spatulate in the
distal half, with beak-like apex (Fig. 1H). Right phallomere:
R2 slightly elongated, round; basal left with a lobated pro-
trusion (Fig. 1G).
Female (n=5): Body length 13.0–13.7 mm; pronotum
length 3.5–3.9 mm, width 6.1–6.5 mm; tegmen length 8.7–
9.0 mm (Fig. 1C, D). Head, pronotum, and tegmen similar
to male in color (Fig. 1K). Dorsum brownish black with
yellow area occupied from 2nd to 5th segments and lateral
sides of 6th segment. Supra-anal plate semicircular in shape
and brownish black. Ventral side of abdomen brownish
black with yellow area occupied from 1st through 6th seg-
ments; subgenital plate brownish black.
Ootheca (n=4): Length 3.0–3.1 mm, width 5.0–6.0 mm.
Light brown, trapezoidal pouch, with fine serration on one
side of the margin with five longitudinal ridges on each side
(Fig. 1P).
Larva (last instar): Male (n=1) body length 11.5 mm,
mesonotum width 7.0 mm (Fig. 1N). Female (n=1) body
length 12.1 mm, mesonotum width 7.5 mm. Body brown,
with many fine bristles (Fig. 1O).
Distribution. Miyako-jima Island, Southwest Japan (Fig.
2).
148 Shizuma Yanagisawa et al.

Fig.  1.  A new species of Eucorydia miyakoensis Yanagisawa, Sakamaki, and Shimano, sp. nov. A–B, male (eM-001 holotype); C–D, female
(eM-004 paratype); E, supra-anal plate; F, right phallomere with L7; G, R2; H, L7; I, genital hook/L3; J, basal tegmina of male (eM-001 holo-
type); K, basal tegmina of female (eM-004 paratype); L, hind wing of male (eM-001 holotype); M, dorsal view of the abdominal segments of
male (eM-001 holotype); N, larva (last instar) male from Gusukube, Miyako-jima Island, 12 November 2019, S. Yanagisawa leg. (no voucher
specimen left); O, larva (last instar) female from Gusukube, Miyako-jima Island, 12 November 2019, S. Yanagisawa leg. (no voucher speci-
men left); P, ootheca (eMo-001). Scale bars: 5 mm.
 A new species of Eucorydia from the Miyako-jima Island 149

Fig.  2.  Distribution of five Eucorydia species from Japan and Taiwan.

Table  3.  Specimen used in molecular phylogenetic analysis, with accession no.

Accession no.
Taxa Sample ID
28S H3 COII 12S 16S
Eucorydia miyakoensis sp. nov. Miyako_01, eM-009 LC565406 LC565393 LC565401 LC565389 LC565385
Miyako_02, eM-010 — LC565394 LC565402 LC565390 LC565386
Miyako_03, eM-011 — LC565395 LC565403 LC565391 LC565387
Miyako_04, eM-012 — LC565396 LC565404 LC565392 LC565388
Eucorydia tokaraensis Akuseki_01, eT-015 LC480910 LC480860 LC480898 LC480840 LC480822
Amami_01, eT-001 LC480909 LC480857 LC480897 LC480837 LC480819
Eucorydia yasumatsui Ishigaki_01, eY-001 LC480907 LC480855 LC480885 LC480843 LC480825
Iriomote_01, eY-006 LC480908 LC480856 LC480888 LC480846 LC480828
Eucorydia donanensis Yonaguni_01, eN-001 LC480911 LC480863 LC480891 LC480849 LC480831
Eucorydia dasytoides “zonata population” Taiwan_01, eD-001 LC480912 LC480864 LC480894 LC480852 LC480834
Eucorydia dasytoides “purpuralis population” MF286954 — MF287042 MF286822 MF286888
Ergaula capucina DQ874214 DQ873966 DQ874280 KF855789 JN615300
Ergaula sp. MF286955 MF286996 MF287075 MF286823 MF286889

Etymology. The new species was named after the sam-
pling site Miyako-jima Island.
Sequences. LC565385–LC565406, including two nuclear
markers (28S and H3) and four mitochondrial markers (12S,
16S, COI, COII) from four specimens (eM-009–eM-012).
Phylogeny and genetic distance. Table 3 lists the se-
quences used for molecular phylogenetic reconstruction, in-
cluding five molecular markers obtained from four individ-
uals of E. miyakoensis sp. nov. ML tree inferred from the five
concatenated markers is shown in Fig. 3 with nodal support
values. Trees resulting from ML and BI had identical topolo-
gies; therefore, only ML tree is presented. The Japanese Eu-
corydia populations formed a sister clade with the E. dasy-
toides population of Taiwan Island. The Japanese popula-
tions were also monophyletic and divided into four lineages.
New species from Miyako-jima Island formed a clade with
E. donanensis from Yonaguni-jima Island.
Table 4 lists pairwise genetic distances of COI sequences
in Japanese Eucorydia species and related species. Although
only a small number of individuals were determined to have
the COI sequence, Japanese Eucorydia species are genetically
homologous on each island, and almost no intraspecific varia-
tion was detected for the COI gene. Only E. yasumatsui, dis-
tributed in Iriomote-jima and Ishigaki-jima Islands, has three
haplotypes in COI sequences. Differences between these hap-
lotypes include one or two substitutions (K2P 0.2%–0.3%).
The new species were separated by genetic distances ranging
from 3.4% to 6.7% (K2P) (Table 3). Similarly, genetic distances
between the Japanese Eucorydia species and the zonata popu-
lation of E. dasytoides were relatively large (K2P 8.7%–10.3%).
150 Shizuma Yanagisawa et al.

Fig.  3.  Maximum likelihood tree inferred from the concatenated five-gene dataset (three mitochondrial 12S rRNA, 16S rRNA, and COII;
and two nucleic 28S rRNA and histone H3 genes). Nodal numbers indicate bootstrap support (BS) values and posterior probabilities (PP).
Asterisk (*) indicates 100% BS and 1.0 PP. Unit of evolutionary distance is the number of base substitutions per site.

Table  4.  Genetic distances among Eucorydia species. K2P distances among COI sequences are shown below the diagonal. Standard error
estimates are shown above the diagonal.

Taxa Accession no. 1 2 3 4 5 6 7 8

1. Eucorydia miyakoensis sp. nov. LC565397– — 0.008 0.008 0.008 0.010 0.009 0.012 0.016
LC565400
2. Eucorydia yasumatsui (Ishigaki) LC480865– 0.036 — 0.002 0.002 0.012 0.010 0.014 0.016
LC480867
3. Eucorydia yasumatsui (Iriomote 01) LC480869 0.036 0.003 — 0.002 0.012 0.010 0.013 0.016
4. Eucorydia yasumatsui (Iriomote 02) LC480868, 0.034 0.002 0.002 — 0.012 0.010 0.013 0.016
LC480870
5. Eucorydia tokaraensis LC480871– 0.067 0.076 0.073 0.075 — 0.010 0.012 0.016
LC480876
6. Eucorydia donanensis LC480877– 0.050 0.057 0.053 0.055 0.060 — 0.013 0.016
LC480879
7. Eucorydia dasytoides “zonata population” LC480880– 0.089 0.103 0.099 0.101 0.087 0.087 — 0.014
LC480882
8. Eucorydia sp. KP986406 0.145 0.155 0.151 0.153 0.143 0.142 0.129 —

Remarks. This species was found only on Miyako-jima
Island (Fig. 2). The presence of this new species was also
suggested in the remarks section which discussed the Euco-
rydia sp. (=E. tokaraensis) in Asahi et al. (2016), but it was
not described as a valid species since there were an insuffi-
cient number of specimens at that time. Obscureness of the
orange band on the tegmina varied among individuals. The
oothecae of this species are similar to the ootheca of E. yasu-
matsui described by Fujita and Machida (2014).
Keys
Keys to Japanese Eucorydia species with similar conge-
ners from adjacent areas; based on Qiu et al. (2017) (E. gui-
linensis, the zonata and purpuralis populations of E. dasy-
toides, and E. yunnanensis Woo, Guo, and Feng, 1986) and
Yanagisawa et al. (2020) (E. yasumatsui, E. donanensis and
E. tokaraensis).
1. Tegmina with pubescence at the basal portion������������� 2
— Tegmina without pubescence on the surface����������������� 3
2. L7 of male genitalia round in the basal half; pubescence
on tegmina orange; overall length less than 13 mm in
male������������������������������������������������ E. miyakoensis sp. nov.
— L7 of male genitalia curved toward left-posterior with
an elongate and sharp process in left base; pubescence
on tegmina yellowish white; overall length more than
13 mm������������������������������������������������������������� E. guilinensis
3. Overall length more than 18 mm in male ��������������������� 4
— Overall length less than 16 mm��������������������������������������� 5
4. Orange band of tegmina twice interrupted. . . . . . . . . . . .
 A new species of Eucorydia from the Miyako-jima Island
������������������������������� E. dasytoides “purpuralis population”
— Orange band of tegmina without interruption. . . . . . . . .
������������������������������������� E. dasytoides “zonata population”
5. Metallic blue tegmina without any markings . . . . . . . . . .
������������������������������������������������������������������������E. yasumatsui
— Metallic blue to green tegmina with orange band and/
or orange spots������������������������������������������������������������������� 6
6. Dorsal side of abdomen dark purple in male . . . . . . . . . .
������������������������������������������������������������������������E. donanensis
— Dorsal side of abdomen with yellow blotch laterally��� 7
7. Tegmina with an absolutely uninterrupted orange band
����������������������������������������������������������������������E. yunnanensis
— Tegmina with an orange band interrupted twice . . . . . . .
������������������������������������������������������������������������E. tokaraensis
Acknowledgements
We appreciate Meses. Morine Igawa and Megumi Yanagi-
sawa, Messrs. Jun Sahara, Junta Terai, and Koki Hayashi,
who helped with the field survey. We would like to thank Dr.
Takuya Kiyoshi (National Museum of Nature and Science,
Tsukuba) for the deposition of type specimens.
This study was supported, in part, by the Kagoshima
University “Establishment of Research and Education Net-
work on Biodiversity and its Conservation in the Satsunan
Islands” project conferred by the Ministry of Education,
Culture, Sports, Science, and Technology in Japan to YS, and
Tokyo Metropolitan University Fund for TMU Strategic Re-
search (Leader: Prof. Noriaki Murakami; FY2020–FY2022)
and Research Funds of the Asahi Glass Foundation to SS.
The authors would like to thank Enago (www.enago.jp) for
the English language review.
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