(Sample_Name = F394F505LckBG_16) GACTGTACTTAGCTGGAGACCGTTTAACTCAATGATGATGATGATGAT GGTCGACGGCGCTATTCAGATCCTCT TCTGAG ATGAGTTTTTGTTCTAGAAAGCTTGGCTGAGGCTGGAACTGGCCCTCT GTGGCCGTGAAGAAGTCCTCCAGCACAC TGCG CAGGTAGTCAAAGGTGGGCCGGTCCTCTGGGCGCTCCTTCCAGCACA GCCTCATGAGTTGGTACAGCTCCTCTGGA CAGT TGTCAGGGCGCACCATGCGGTAGCCTCGCTCCAGGTTCTGAATCACC TCCGGGTTGGTCATCCCTGGGTAAGGGAT GCGG CCGTGGGTGACAATTTCCGTCAGCAGGATCCCAAAAGACCACACATC

TGACTTGATGGTGAATGTCCCGTAGTTAA TGGC TTCTGGCGCTGTCCACTTAATGGGAAACTTGGCCCCCTCCCTGGCTGT GAACTCGTTGTCCTCAATGAGGCGTGCT AGGC CAAAGTCTGCAATCTTGCAGCTCAGGGTGTCAGACACCAGAATGTTG GCAGCCCGAAGGTCACGATGAATATAATT CCGC TCTTCAATGAATGCCATGCCTTCTGCAATTTGGGCTGCCATGTCCAGG AGTTTGTTGATGGTCAACTTGATGCCTG AAGG GGTCTTGAGAAAATCCACTAGACTCCCATTCTCCATGTATTCAGTGAT GATGTAGATGGGCTCCTGGGTGACCACA GCGT AGAGCCGAACCAGCCGCTGGTGTTGCAGCTGCTTCATGAGGTTGGCC TCGGCCAGGAAGGCGTTCGGGGACATGCT GCCC TGCTTCAGCACTTCACCGCCACCTTCGTGTGCCCGTTGTAGTACCCCA TCCACACCTCCCCGAACTGTCCAGCCCC CAGC CGCTCCACCAGCTTCAGCGTCTCCCTGGGAACCTCCCACTCGTCCTCC CACACGGCTTCTGGGGCTTCTGGGTCTG GCAG GGCGCTCACGTGTGCACAGCCCATCTGAGCATGTGTATGCGACAGTC ATGCAGCGGAAAGTGATCGAGGGAGATGT AGAG CACGTGTCAGATACGATCTGTATGTTCACCACCTCTCCTGTCTGTCGA

GTCCGACGACGTGAAACGATCCCGTGCT CTCG CCTCTCCGATGAAGAAAGACTAAGTTTCGGGCAGATGCGCTTCCGGT CTTGCGCTCAGCCTGAACGGTTCGCTCAC ATGG TTCATGCAATAGGACCTTGCCGTGAGGAACTTCAATCGGTTCTTGA > (5' --> 3' complementary sequence) - 1246 nucleotides tcaagaaccgattgaagttcctcacggcaaggtcctattgcatgaaccat gtgagcgaaccgttcaggctgagcgcaagaccggaagcgcatctgcccga aacttagtctttcttcatcggagaggcgagagcacgggatcgtttcacgt cgtcggactcgacagacaggagaggtggtgaacatacagatcgtatctga cacgtgctctacatctccctcgatcactttccgctgcatgactgtcgcat acacatgctcagatgggctgtgcacacgtgagcgccctgccagacccaga agccccagaagccgtgtgggaggacgagtgggaggttcccagggagacgc tgaagctggtggagcggctgggggctggacagttcggggaggtgtggatg gggtactacaacgggcacacgaaggtggcggtgaagtgctgaagcagggc agcatgtccccgaacgccttcctggccgaggccaacctcatgaagcagct gcaacaccagcggctggttcggctctacgctgtggtcacccaggagccca tctacatcatcactgaatacatggagaatgggagtctagtggattttctc aagaccccttcaggcatcaagttgaccatcaacaaactcctggacatggc agcccaaattgcagaaggcatggcattcattgaagagcggaattatattc atcgtgaccttcgggctgccaacattctggtgtctgacaccctgagctgc aagattgcagactttggcctagcacgcctcattgaggacaacgagttcac agccagggagggggccaagtttcccattaagtggacagcgccagaagcca ttaactacgggacattcaccatcaagtcagatgtgtggtcttttgggatc ctgctgacggaaattgtcacccacggccgcatcccttacccagggatgac caacccggaggtgattcagaacctggagcgaggctaccgcatggtgcgcc ctgacaactgtccagaggagctgtaccaactcatgaggctgtgctggaag gagcgcccagaggaccggcccacctttgactacctgcgcagtgtgctgga ggacttcttcacggccacagagggccagttccagcctcagccaagctt tc tagaacaaaaactcatctcagaagaggatctgaatagcgccgtcgaccat catcatcatcatcattgagttaaacggtctccagctaagtacagtc
>F394F505-BGH Translated - Frame 1 SRTD*SSSRQGPIA*TM*ANRSG*AQDRKRICPKLSLSSSERREHGIVSRRRTRQTGEVV NIQIVSDTCS TSPSITFRCMTVAYTCSDGLCTRERPARPRSPRSRVGGRVGGSQGDAEAGGAAGGWT VRGGVDGVLQRAH EGGGEVLKQGSMSPNAFLAEANLMKQLQHQRLVRLYAVVTQEPIYIITEYMENGSLVDF LKTPSGIKLTI

NKLLDMAAQIAEGMAFIEERNYIHRDLRAANILVSDTLSCKIADFGLARLIEDNEFTAREG AKFPIKWTA PEAINYGTFTIKSDVWSFGILLTEIVTHGRIPYPGMTNPEVIQNLERGYRMVRPDNCPEEL YQLMRLCWK ERPEDRPTFDYLRSVLEDFFTATEGQFQPQP

(Sample_Name = F394F505LckT7_15) ACAAGCTGGCTGCGTTTACGGGCCCTCTAGA CTCGAGACC ATGGGCTGTGGCCGCAGCTCACACCCGGAAGATG ACTGGA TGGAAAACATCGATGTGTGTGAGAACTGCCATTATCCCATAGTCCCAC TGGATGGCAAGGGCACGCTGCTCATCCG AAAT GGCTCTGAGGTGCGGGACCCACTGGTTACCTACGAAGGCTCCAATCC GCCGGCTTCCCCACTGCAAGACAACCTGG TTAT CGCTCTGCACAGCTATGAGCCCTCTCACGACGGAGATCTGGGCTTTG AGAAGGGGGAACAGCTCCGCATCCTGGAG CAGA GCGGCGAGTGGTGGAAGGCGCAGTCCCTGACCACGGGCCAGGAAG GCTTCATCCCCTTCAATTTTGTGGCCAAAAC GAAC AGCCTGGAGCCCGAACCCTGGTTCTTCAAGAACCTGAGCCGCAAGGA CGCGGAGCGGCAGCTCCTGGCGCCCGGGA ACAC TCACGGCTCCTTCCTCATCCGGGAGAGCGAGAGCACCGCGGGATCGT TTTCACTGTCGGTCCGGGACTTCGACCAG AACC AGGGAGAGGTGGTGAAACATTACAAGATCCGTAATCTGGACAACGGT GGCTTCTACATCTCCCCTCGAATCACTTT TCCC GGCCTGCATGAACTGGTCCGCCATTACACCAATGCTTCAGATGGGCT GTGCACACGGTTGAGCCGCCCCTGCCAGA CCCA GAAGCCCCAGAAGCCGTGGTGGGAGGACGAGTGGGAGGTTCCCAGG GAGACGCTGAAGCTGGTGGAGCGGCTGGGG GCTG GACAGTTCGGGGAGGTGTGGATGGGGTACTACAACGGGCACACGAA

GGTGGCGGTGAAGTGCCTGAAGCAGGCAGC ATGT CCCCGAACGCCTTCCTGGCCGAGGCCAACCTCATGAAGCAGCTGCAT GACCAGCGGCTGGTTCGGCTCTACGCTGT GGTC ACCCAGGAGCCCATCTACATCATCACTGATACATGAGATGGGAGTCTA GTGATTTTCTCAGACCCCTCAGCATCAG TGAC ATCACAACTCTGACATGCAGCCAAATGCAGAGCATGCATTCAGTGAG AGCGATTAATCATCGTGACTCGGCTGCAA CATT CTGGGTCTGACCCCTGACTGCAGATGCGACTGCTAGCAGCCATGAGA CACGAGTCACGCGGAGGGCCAGTCTAATG ACCG CCCGAGCATAATCCGAATTCCATATCAATGTGGGCTTTGTGTATCTCT GTGCGAAAGTAATACG
>F394F505Lck-T7 Translated - Frame 1 MGCGRSSHPEDDWMENIDVCENCHYPIVPLDGKGTLLIRNGSEVRDPLVTYEGSNPPA SPLQDNLVIALH SYEPSHDGDLGFEKGEQLRILEQSGEWWKAQSLTTGQEGFIPFNFVAKTNSLEPEPWFF KNLSRKDAERQ LLAPGNTHGSFLIRESESTAGSFSLSVRDFDQNQGEVVKHYKIRNLDNGGFYISPRITFP GLHELVRHYT NASDGLCTRLSRPCQTQKPQKPWWEDEWEVPRETLKLVERLGAGQFGEVWMGYYNG HTKVAVKCLKQAAC PRTPSWPRPTS*SSCMTSGWFGSTLWSPRSPSTSSLIHEMGV**FSQTPQHQ*HHNSD MQPNAEHAFSES D*SS*LGCNILGLTPDCRCDC*QP*DTSHAEGQSNDRPSIIRIPYQCGLCVSLCESNT

(Sample_Name = F394F505LckBG_16) GACTGTACTTAGCTGGAGACCGTTTAACTCAATGATGATGATGATGA TGGTCGACGGCGCTATTCAGATCCTCT TCTGAG ATGAGTTTTTGTTCTAGAAAGCTTGGCTGAGGCTGGAACTGGCCCTCT GTGGCCGTGAAGAAGTCCTCCAGCACAC TGCG CAGGTAGTCAAAGGTGGGCCGGTCCTCTGGGCGCTCCTTCCAGCACA GCCTCATGAGTTGGTACAGCTCCTCTGGA CAGT TGTCAGGGCGCACCATGCGGTAGCCTCGCTCCAGGTTCTGAATCACC

TCCGGGTTGGTCATCCCTGGGTAAGGGAT GCGG CCGTGGGTGACAATTTCCGTCAGCAGGATCCCAAAAGACCACACATC TGACTTGATGGTGAATGTCCCGTAGTTAA TGGC TTCTGGCGCTGTCCACTTAATGGGAAACTTGGCCCCCTCCCTGGCTGT GAACTCGTTGTCCTCAATGAGGCGTGCT AGGC CAAAGTCTGCAATCTTGCAGCTCAGGGTGTCAGACACCAGAATGTTG GCAGCCCGAAGGTCACGATGAATATAATT CCGC TCTTCAATGAATGCCATGCCTTCTGCAATTTGGGCTGCCATGTCCAGG AGTTTGTTGATGGTCAACTTGATGCCTG AAGG GGTCTTGAGAAAATCCACTAGACTCCCATTCTCCATGTATTCAGTGAT GATGTAGATGGGCTCCTGGGTGACCACA GCGT AGAGCCGAACCAGCCGCTGGTGTTGCAGCTGCTTCATGAGGTTGGCC TCGGCCAGGAAGGCGTTCGGGGACATGCT GCCC TGCTTCAGCACTTCACCGCCACCTTCGTGTGCCCGTTGTAGTACCCCA TCCACACCTCCCCGAACTGTCCAGCCCC CAGC CGCTCCACCAGCTTCAGCGTCTCCCTGGGAACCTCCCACTCGTCCTCC CACACGGCTTCTGGGGCTTCTGGGTCTG GCAG GGCGCTCACGTGTGCACAGCCCATCTGAGCATGTGTATGCGACAGTC ATGCAGCGGAAAGTGATCGAGGGAGATGT AGAG CACGTGTCAGATACGATCTGTATGTTCACCACCTCTCCTGTCTGTCGA GTCCGACGACGTGAAACGATCCCGTGCT CTCG CCTCTCCGATGAAGAAAGACTAAGTTTCGGGCAGATGCGCTTCCGGT CTTGCGCTCAGCCTGAACGGTTCGCTCAC ATGG TTCATGCAATAGGACCTTGCCGTGAGGAACTTCAATCGGTTCTTGA > (5' --> 3' complementary sequence) - 1246 nucleotides tcaagaaccgattgaagttcctcacggcaaggtcctattgcatgaaccat gtgagcgaaccgttcaggctgagcgcaagaccggaagcgcatctgcccga aacttagtctttcttcatcggagaggcgagagcacgggatcgtttcacgt

cgtcggactcgacagacaggagaggtggtgaacatacagatcgtatctga cacgtgctctacatctccctcgatcactttccgctgcatgactgtcgcat acacatgctcagatgggctgtgcacacgtgagcgccctgccagacccaga agccccagaagccgtgtgggaggacgagtgggaggttcccagggagacgc tgaagctggtggagcggctgggggctggacagttcggggaggtgtggatg gggtactacaacgggcacacgaaggtggcggtgaagtgctgaagcagggc agcatgtccccgaacgccttcctggccgaggccaacctcatgaagcagct gcaacaccagcggctggttcggctctacgctgtggtcacccaggagccca tctacatcatcactgaatacatggagaatgggagtctagtggattttctc aagaccccttcaggcatcaagttgaccatcaacaaactcctggacatggc agcccaaattgcagaaggcatggcattcattgaagagcggaattatattc atcgtgaccttcgggctgccaacattctggtgtctgacaccctgagctgc aagattgcagactttggcctagcacgcctcattgaggacaacgagttcac agccagggagggggccaagtttcccattaagtggacagcgccagaagcca ttaactacgggacattcaccatcaagtcagatgtgtggtcttttgggatc ctgctgacggaaattgtcacccacggccgcatcccttacccagggatgac caacccggaggtgattcagaacctggagcgaggctaccgcatggtgcgcc ctgacaactgtccagaggagctgtaccaactcatgaggctgtgctggaag gagcgcccagaggaccggcccacctttgactacctgcgcagtgtgctgga ggacttcttcacggccacagagggccagttccagcctcagccaagctttc tagaacaaaaactcatctcagaagaggatctgaatagcgccgtcgaccat catcatcatcatcattgagttaaacggtctccagctaagtacagtc

(Sample_Name = F394F505LckT7_15) ACAAGCTGGCTGCGTTTACGGGCCCTCTAGACTCGAGACCATGGGCT GTGGCCGCAGCTCACACCCGGAAGATG ACTGGA TGGAAAACATCGATGTGTGTGAGAACTGCCATTATCCCATAGTCCCAC TGGATGGCAAGGGCACGCTGCTCATCCG AAAT GGCTCTGAGGTGCGGGACCCACTGGTTACCTACGAAGGCTCCAATCC GCCGGCTTCCCCACTGCAAGACAACCTGG TTAT CGCTCTGCACAGCTATGAGCCCTCTCACGACGGAGATCTGGGCTTTG AGAAGGGGGAACAGCTCCGCATCCTGGAG CAGA GCGGCGAGTGGTGGAAGGCGCAGTCCCTGACCACGGGCCAGGAAG GCTTCATCCCCTTCAATTTTGTGGCCAAAAC GAAC AGCCTGGAGCCCGAACCCTGGTTCTTCAAGAACCTGAGCCGCAAGGA

CGCGGAGCGGCAGCTCCTGGCGCCCGGGA ACAC TCACGGCTCCTTCCTCATCCGGGAGAGCGAGAGCACCGCGGGATCGT TTTCACTGTCGGTCCGGGACTTCGACCAG AACC AGGGAGAGGTGGTGAAACATTACAAGATCCGTAATCTGGACAACGGT GGCTTCTACATCTCCCCTCGAATCACTTT TCCC GGCCTGCATGAACTGGTCCGCCATTACACCAATGCTTCAGATGGGCT GTGCACACGGTTGAGCCGCCCCTGCCAGA CCCA GAAGCCCCAGAAGCCGTGGTGGGAGGACGAGTGGGAGGTTCCCAGG GAGACGCTGAAGCTGGTGGAGCGGCTGGGG GCTG GACAGTTCGGGGAGGTGTGGATGGGGTACTACAACGGGCACACGAA GGTGGCGGTGAAGTGCCTGAAGCAGGCAGC ATGT CCCCGAACGCCTTCCTGGCCGAGGCCAACCTCATGAAGCAGCTGCAT GACCAGCGGCTGGTTCGGCTCTACGCTGT GGTC ACCCAGGAGCCCATCTACATCATCACTGATACATGAGATGGGAGTCTA GTGATTTTCTCAGACCCCTCAGCATCAG TGAC ATCACAACTCTGACATGCAGCCAAATGCAGAGCATGCATTCAGTGAG AGCGATTAATCATCGTGACTCGGCTGCAA CATT CTGGGTCTGACCCCTGACTGCAGATGCGACTGCTAGCAGCCATGAGA CACGAGTCACGCGGAGGGCCAGTCTAATG ACCG CCCGAGCATAATCCGAATTCCATATCAATGTGGGCTTTGTGTATCTCT GTGCGAAAGTAATACG
Trial 2

(Sample_Name = F394F505LckT7_15) CCAGGGTAGCAGCGTTACGGGCCTCTAGACTCGAGACCATGGGCTG TGGCCGCAGCTCACACCCGGAAGATGAC TGGATG GAAAACATCGATGTGTGTGAGAACTGCCATTATCCCATAGTCCCACTG GATGGCAAGGGCACGCTGCTCATCCGAA ATGG CTCTGAGGTGCGGGACCCACTGGTTACCTACGAAGGCTCCAATCCGC

CGGCTTCCCCACTGCAAGACAACCTGGTT ATCG CTCTGCACAGCTATGAGCCCTCTCACGACGGAGATCTGGGCTTTGAG AAGGGGGAACAGCTCCGCATCCTGGAGCA GAGC GGCGAGTGGTGGAAGGCGCAGTCCCTGACCACGGGCCAGGAAGGCT TCATCCCCTTCAATTTTGTGGCCAAAACGA ACAG CCTGGAGCCCGAACCCTGGTTCTTCAAGAACCTGAGCCGCAAGGACG CGGAGCGGCAGCTCCTGGCGCCCGGGAAC ACTC ACGGCTCCTTCCTCATCCGGGAGAGCGAGAGCACCGCGGGATCGTTT TCACTGTCGGTCCGGGACTTCGACCAGAA CCAG GGAGAGGTGGTGAAACATTACAAGATCCGTAATCTGGACAACGGTGG CTTCTACATCTCCCCTCGAATCACTTTTC CCGG CCTGCATGAACTGGTCCGCCATTACACCAATGCTTCAGATGGGCTGT GCACACGGTTGAGCCGCCCCTGCCAGACC CAGA AGCCCCAGAAGCCGTGGTGGGAGGACGAGTGGGAGGTTCCCAGGGA GACGCTGAAGCTGGTGGAGCGGCTGGGGGC TGGA CAGTTCGGGGAGGTGTGGATGGGGTACTACAACGGGCACACGAAGG TGGCGGTGAAGTGCCTGAAGCAGGCAGCAT GTCC CCGAACGCCTTCCTGGCCGAGGCCAACCTCATGAAGCAGCTGCAACA CCAGCGGCTGGTTCGGCTCTACGCTGTGG TCAC CCAGGAGCCCATCTACATCATCACTGATACATGAGAATGGGAGTCTA GTGATTTTCTCAGACCCCTCAGCATCAAG TGAC ATCAACAAACTCTGACATGCAGCCAAATGCAGAAGCATGCATCATGA GGCGGAATTATATTCATCGTGACTCGGCC TGCC AACATTCTGTGTGTCTGACACCTGGAGCTGAGAATGGCGACTGGCCT ACACACGCTCATTGAGAACACAAGTTCCA CGCG CGGGAGGGGCTATTCTATTGGACGCGCCGAGATCCTAATCGGCATAC TTCATCAAAGGTGGGGCTCTTGTGTAC Conclusion: hLck, Y394F, Y505F, T7 shows C5R, A119T, S274C,

G278A, S279A. BGH shows D283N.