TABLE OF CONTENTS

Page

1.0 1.1 1.2 1.3 1.4

INTRODUCTION Background of study Problem statement Significance of study Objectives of the study

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2.0 2.1 2.2

2.3

LITERATURE REVIEW Enzyme Lipase 2.2.1 General structure of lipase 2.2.2 Sources of lipase 2.2.3 Industrial applications of microbial lipase 2.2.3.1 Food industry 2.2.3.2 Detergents 2.2.3.3 Cosmetics and perfumery 2.2.3.4 Leather industry 2.2.3.5 Environmental management 2.2.3.6 Biosensors 2.2.3.7 Pharmacy Lipase assay 2.3.1 Titrimetric assay

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3.0 3.1

3.2

METHODOLOGY Materials 3.1.1 Raw materials 3.1.2 Chemicals 3.1.3 Apparatus Methods 3.2.1 Sample collections 3.2.2 Isolation of lypolytic bacteria 3.2.3 Screening for lipase activity of isolates 3.2.4 Culturing and characterization of isolates 3.2.4.1 Gram staining and biochemical tests 3.2.5 Lipase assay 3.2.5.1 Titrimetric method

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3.3

Data analysis 3.3.1 Graph of lipolytic activity 3.3.2 Morphology of isolates

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CITED REFERENCES GANTT CHART STUDENT-SUPERVISOR MEETING FORM

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