Partition Chromatography

It is also known as Liquid-Liquid Chromatography (LLC). If the mobile phrase is gas then it is called Gas-liquid Chromatography (GLC). In LLC the two phase are liquids. The two liquids must be immiscible. The stationary liquid is present as thin film on an inert solid support and it is usually the more polar liquid (aqueous phase). Separation accurse due to the difference in partition coefficients of solutes between the two liquids.

Examples of supports: 1- Filter paper. 2- Cellulose powder. 3- Kieselguhr . 4-Silica gel.

These supports the aqueous stationary phase by hydrogen bonding.

Reversed phase Chromatography:

The support is modified to hold the non polar stationary phase and the mobile phase is the more polar liquid in this case. Reversed phase silica gel is an example of the stationary phases in this type such as RP18 (C18) / RP 8 (C8).

Partition Chromatography is better for the separation of more polar solutes that may not easily eluted from the systems based adsorption.