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Contents
1. Reversed-Phase Column
1.1 ODS Column
1.2 Other Silica-Based Column
1.3 Polymer-Based Column
2. Normal-Phase Column
3. Hydrophilic Interaction Chromatography Column
4. Summary of Shodex Partition/Adsorption Columns
1. Reversed-Phase Column
1.1 ODS column
The packing material used for reversed-phase column is often made of silica gel modified with functional group.
In the story of after work activities (Lesson 1), people may go for a drink by knowing the bar, but if there is a
promoter standing in front of the bar, handing out flyers, more people may stop by for a drink. This promoter is
the functional group in RP column. The most often used functional group is octadecyl. Octadecyl functional
group is a straight carbon chain of 18. Its molecular structure is shown below.
It is also often called C18 (C eighteen) column. The name of octadecyl is originated from octa meaning number
8 and deci meaning number 10. A numerous number of this hydrocarbon chain is attached on the surface of
silica gel. By looking at the structure written above, it may look like a very long chain, but compare to the size
of silica gel, it is actually small. Thus, an infinite number of chains can be modified on one silica gel. More over
these chains are modified even inside of the silica-gel pores.
Back to our "famous" story, some people may pick-up the flyer, but others may not pay any attention. Similarly,
some compounds may "stopped" by the functional group, but others may not. This defines the eluting timing of
each component. By using more scientific words, it can be said "a separation is a result of different affinity
between the gel and the components in the sample". This affinity is called "partition" or "adsorption" thus this
type of separation is also called partition/adsorption chromatography. Precisely speaking, reversed phase mode
is a part of partition/adsorption mode.
The gel made of silica-base and modified with octadecyl functional group is called octadecyl silica (ODS) gel.
Also the column packed with ODS gel is called ODS column (or called C18 column). Among the silica-based
column available in the market, about 80% of them are ODS columns.
Ideally, entire surface of ODS gel is modified with C18 functional group; however there will be remaining spaces
that are not modified. Those part are called "residual silanol" and the presence of residual silanols can influence
the separation. Often "end capping" is applied to the gels to immobilize the residual silanol. Almost all ODS
columns nowadays are end-capped, however depending on the type of analyte, presence of silanol may provide
better separation results.
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3/11/2019 Lesson 3: Partition/Adsorption Chromatography Columns | Shodex/ HPLC Columns, Detectors, Standards
is ODP series. As you may guess, the name of ODP came from OctaDecyl Polymer. We believe it is easy to
remember, as S (silica) in ODS was simply replaced with P (polymer). The next popular polymer-based RP
column is DE-413. The packing gel is made of polymethacrylate. What unique about this gel is that is not
modified with any functional group. Instead of using the characteristics of modified functional group, the
interaction occurs between the sample and the natural characteristics of polymethacrylate. The question about
selection of ODP vs. DE-413 is depends on the sample, you may find many applications on our website to see
when ODP/DE-413 is suitable.
2. Normal-Phase Column
It is rather complicated to explain detailed-theoretical differences between RP and normal phase, thus we will
keep it simple here as an introduction. Gels and mobile phases used for HPLC analysis have different polarities.
Water and oil is a famous example of something does not mix: Water is categorized as something with high
polarity while oil is categorized as something with low polarity. Oil is a type of carbohydrate, made of carbon
and hydrogen; such compound has low polarity. In contrast, water is made of oxygen and hydrogen; such
compound has higher polarity. Silica gel without modification has high polarity, but when C18 functional group is
modified, the polarity becomes low. RP mode uses gel with low polarity (e.g., ODS) and mobile phase with high
polarity (e.g., water, acetonitrile). Normal-phase mode uses gel with high polarity (e.g. silica) and mobile phase
with low polarity (e.g. hexane, chloroform). Instead of using the word low or high polarity, it is also common to
use words, hydrophilic or hydrophobic. Something easy to dissolve in water (i.e., high polarity) is called
hydrophilic and something easy to dissolve in oil (i.e., low polarity) is called hydrophobic.
At the very early stage of HPLC development, silica gel without any functional group was only used. Thus,
historically normal-phase mode was developed first and so named "Normal". Then the separation mode which
uses opposite separation theory to normal phase was developed and named "Reversed". RP mode is much more
popularly used than normal phase nowadays, but we cannot change the historical background, and thus they
are still called normal and reversed-phase modes.
Figure 1
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