April 16 SEARCH FOR A GRAM NEGATIVE BACTERIA THAT CAUSE SKIN DISEASE

Severe skin infection may occur in patients Pseudomonas aeruginosa bacteremia. The bacterium is the second most common cause of burn wound infections in hospitalized patients.

April 17-18 ANTIMICROBIAL PEPTIDES FROM MARINE INVERTEBRATES

Group Tunicates

Species Styela clava

Styela plicata Halocynthia aurantium Halocynthia roretzi Crustaceans Penaeus vannemi

Litopenaeus vannamei Litopenaeus setiferus Carcinus maenas Callinectes sapidus Chelicerates Tachyplesus tridentatus Limulus polyphemus

Tachyplesus gigas Mollusks Mytilis edulis

Mytilis galloprovincialis

Peptide Styelin Clavanin Clavaspirin Plicatamide Dicynthaurin Halocidin Halocyamines Penaeidins 1 to 3 2.7, 7.9, and 8.3 kDa Lv1 to 6 Ls1 to 3 Penaeidin 4 6.5 kDa Crustin Cm-1 Callinectin Tachyplesin I Big defensin Tachyplesin II Polyphemusin I and II Tachyplesin III Defensins A and B Mytilin A and B Mytimicin Myticin A and B Defensins 1 and 2 Mytilin B, C, D, and G1

April 19 - STATEMENT OF THE PROBLEM

The study determined the antibacterial activity of ________________ against Staphylococcus aureus and Pseudomonas aeruginosa. Specifically, the study aimed to answer the following questions: 1. What is the minimum inhibitory concentration (MIC) of________________ extract against Staphylococcus aureus and Pseudomonas aeruginosa? 2. What is the level of antibacterial activity using the mean zone of inhibition of ____________extract against Staphylococcus aureus and Pseudomonas aeruginosa? 3. What is the level of antibacterial activity using the mean zone of inhibition of the formulated ____________ cream against Staphylococcus aureus and Pseudomonas aeruginosa? 4. Is the formulated ____________ cream safe for current application?

April 20 - METHODOLOGY Note: Sir we did not improve our methodology yet since we have not decided what marine creatures are to be used. We will include the Methodology on the next update Thank you Sir!