RECOMMENDED METHODS OF ANALYSIS AND SAMPLING CODEX STAN 234-19991 PART A METHODS OF ANALYSIS BY COMMODITY CATEGORIES AND NAMES

PART B METHODS OF SAMPLING BY COMMODITY CATEGORIES AND NAMES Table of Contents All Foods Cereals, Pulses and Legumes and Derived Products Cocoa Products and Chocolate Fats and Oils and Related Products Fish and Fishery Products Foods for Special Dietary Uses Fruit Juices Milk and Milk Products Natural Mineral Waters Processed Fruits and Vegetables Processed Meat and Poultry Products and Soups and Broths Quick Frozen Fruits and Vegetables Sugars and Honey Miscellaneous Products

The most updated version of the method should be used, in application of ISO/IEC 17025: 1999. The present list of methods reflects the amendments adopted by the 34th Session of the Codex Alimentarius Commission in 2011.

PART A METHODS OF ANALYSIS BY COMMODITY CATEGORIES AND NAMES

Commodity All Foods All foods All foods All foods All foods All Foods (see also meat products) Individual Foods2 Individual Foods3

Provision

Method

Principle High performance liquid chromatography High performance liquid chromatography Spectrophotometry High performance liquid chromatography Part 1- General considerations Part 1: Optimized Monier-Williams method Part 2: Enzymatic method

Type II II III III N/A III III

Acesulfame K, Aspartame Cyclamate Cyclamate Saccharin Nitrates and/or Nitrites Sulphites Sulphites

EN 12856 : 1999-04 EN 12857 : 1999-04 NMKL 123 (1998) EN 12856 : 1999-04 EN 12014-1:1997-04 EN 1988-1 : 1998-02 AOAC 990.28 EN 1988-2:1998 -02 NMKL 135 (1990) ISO 665:1977 (confirmed 1995) AOAC 923.03 ISO 2171:1993 ICC Method No 104/1 (1990) AOAC 945.38F; 920.39C

Cereals, Pulses and Legumes and Derived Products Certain pulses Degermed maize (corn) meal and maize (corn) grits Degermed maize (corn) meal and maize (corn) grits Moisture Ash Gravimetry Gravimetry I I

Fat, crude

Gravimetry (ether extraction)

2 3

Hominy, fruit juice, sea food Wine, dried apples, lemon juice, potato flakes, sultanas, beer

Degermed maize (corn) meal and maize (corn) grits Degermed maize (corn) meal and maize (corn) grits Degermed maize (corn) meal and maize (corn) grits Durum wheat semolina and durum wheat flour Durum wheat semolina and durum wheat flour Durum wheat semolina and durum wheat flour Instant Noodles Instant Noodles Instant Noodles Maize (corn) Peanuts (raw) Peanuts (raw) Peanuts (intended for further processing) Peanuts (Cereals, shell-fruits and derived products ( including peanuts)) Peanuts (intended for further processing) Pearl millet flour

Moisture Particle size (granularity) Protein Ash (semolina) Moisture Protein (N x 5.7) Extraction of oil from instant noodles Acid Value Moisture Moisture Aflatoxins, total Aflatoxins, total Aflatoxins, total Sum of aflatoxins B1, B2, G1 and G2 Aflatoxins, total Ash

ISO 712:1998 ICC Method No 110/1 (1986) AOAC 965.22 ICC Method No 105/1 (1986) AOAC 923.03 ISO 2171:1993 ISO 712:1998 ICC Method 110/1 (1986) ICC Method No 105/1 described in the standard described in the standard described in the standard ISO 6540:1980 (confirmed 1994) AOAC 991.31 AOAC 993.17 AOAC 975.36 EN 12955 : 1999-07 ISO 16050:2003 AOAC 979.18 AOAC 923.03

Gravimetry Sieving Titrimetry, Kjeldahl digestion Gravimetry Gravimetry Titrimetry, Kjeldahl digestion Gravimetry Titrimetry Gravimetry Gravimetry Immunoaffinity column (Aflatest) Thin layer chromatography Romer minicolmn HPLC with post column derivatization and immunoaffinity column clean up Holaday-Velasco minicolumn Gravimetry

I I I I I I I I I I II III III

III III I

Pearl millet flour

Colour

Modern Cereal Chemistry, 6th Ed., D.W. Kent-Jones and A.J. Amos (Ed.), pp. 605612, Food Trade Press Ltd, London, 1969. AOAC 945.38F; 920.39C ISO 5498:1981 (B.5 Separation) ISO 712:1998 ICC Method No 110/1 (1986) AOAC 920.87 AOAC 923.03 ISO 2171:1993 ICC Method No 104/1 (1990) Modern Cereal Chemistry, 6th Ed., D.W. Kent-Jones and A.J. Amos (Ed.), pp. 605612, Food Trade Press Ltd, London, 1969. AOAC 945.38F; 920.39C ICC Method No 113 (1972) ISO 6541:1981 (confirmed 1996) ISO 712:1998 ICC Method No 110/1 (1986) AOAC 965.22 ICC Method No 105/1 (1986) ISO 9648:1988 (confirmed 1994) AOAC 923.03 ISO 2171:1993 ICC Method No 104/1 (1990) AOAC 945.38F, 920.39C ISO 6540:1980 (confirmed 1994) ICC Method No 105/1 (1986)

Colorimetry using specific colour grader Gravimetry (ether extraction) Gravimetry Gravimetry Titrimetry, Kjeldahl digestion Gravimetry

IV

Pearl millet flour Pearl millet flour Pearl millet flour Pearl millet flour Sorghum flour

Fat, crude Fibre, crude Moisture Protein Ash

I I I I I

Sorghum flour

Colour

Colorimetry using specific colour grader Gravimetry (ether extraction) Gravimetry Gravimetry Sieving Titrimetry, Kjeldahl digestion Spectrophotometry Gravimetry

IV

Sorghum flour Sorghum flour Sorghum flour Sorghum flour Sorghum flour Sorghum flour Sorghum grains

Fat, crude Fibre, crude Moisture Particle size (granularity) Protein Tannins Ash

I I I I I I I

Sorghum grains Sorghum grains Sorghum grains

Fat, crude Moisture Protein

Gravimetry (ether extraction) Gravimetry Titrimetry, Kjeldahl digestion

I I I

Sorghum grains Soy protein products Soy protein products Soy protein products Soy protein products Soy protein products Vegetable protein products Vegetable protein products Vegetable protein products Vegetable protein products Vegetable protein products Wheat flour

Tannins Ash Fat Fibre, crude Moisture Protein Ash Fat Fibre, crude Moisture Protein Ash

ISO 9648:1988 (confirmed 1994) AOAC 923.03 ISO 2171:1993 (Method B) CAC/RM 55-1976 - Method 1 ISO 5498:1981 AOAC 925.09 AOAC 955.04D (using factor 6.25) AOAC 923.03 ISO 2171:1993 (Method B) CAC/RM 55-1976 - Method 1 AACC (1982) 32-17 AOAC 925.09 AOAC 955.04D (using factor 6.25) AOAC 923.03 ISO 2171:1993 ICC Method No 104/1 (1990) AOAC 939.05 ISO 712:1998 ICC Method No 110/1 (1986) AOAC 965.22 ICC Method No 105/1 (1986) Vital wheat gluten and devitalized wheat gluten AOAC 979.09 (wheat protein in grain Nx5.7) Solubilized wheat protein AOAC 920.87 (wheat protein in flour Nx5.7)

Spectrophotometry Gravimetry Gravimetry (extraction) Gravimetry Gravimetry (vacuum oven) Titrimetry , Kjeldahl digestion Gravimetry, Direct Gravimetry (extraction) Ceramic fiber filteration Gravimetry (vacuum oven) Titrimetry, Kjeldahl digestion Gravimetry

I I I I I II I I I I II I

Wheat flour Wheat flour Wheat flour Wheat flour Wheat protein products including wheat gluten

Fat acidity Moisture Particle size (granularity) Protein Protein

Titrimetry Gravimetry Sieving Titrimetry, Kjeldahl digestion Kjeldahl

I I I I I

Kjeldahl

Wheat protein products including Wheat gluten Wheat protein products including Wheat gluten Whole and decorticated pearl millet grains Whole and decorticated pearl millet grains Whole and decorticated pearl millet grains Whole and decorticated pearl millet grains Whole and decorticated pearl millet grains Whole maize (corn) meal

Fibre, crude Ash

AOAC 962.09 AOAC 923.03 ISO 2171:1980, method B AOAC 923.03 AOAC 945.38F; 920.39C ISO 5498:1981 (B.5 Separation) ISO 712:1998 ICC Method No 110/1 (1986) AOAC 920.87 AOAC 923.03 ISO 2171:1993 ICC Method No 104/1 (1990) AOAC 945.38F; 920.39C ISO 712:1998 ICC Method No 110/1 (1986) AOAC 965.22 ICC Method No 105/1 (1986)

Ceramic fiber filteration Gravimetry

I I

Ash Fat, crude Fibre, crude Moisture Protein Ash

Gravimetry Gravimetry (ether extraction) Gravimetry Gravimetry Titrimetry, Kjeldahl digestion Gravimetry

I I I I I I

Whole maize (corn) meal Whole maize (corn) meal Whole maize (corn) meal Whole maize (corn) meal Cocoa Products and Chocolate Chocolate and chocolate products Chocolate and chocolate products Chocolate and chocolate products

Fat, crude Moisture Particle size (granularity) Protein

Gravimetry (ether extraction) Gravimetry Sieving Titrimetry, Kjeldahl digestion

I I I I

Cocoa butter Fat-free cocoa solids Fat-free milk solids

AOAC 963.15 IOCCC 14-1972 AOAC 931.05 IOCCC 17-1973 or AOAC 939.02

Gravimetry (Soxhlet extraction) Oven evaporation and factor Titrimetry, Kjeldahl digestion; after extraction of milk proteins

I I II

Chocolate and chocolate products Chocolate and chocolate products Chocolate and chocolate products

Fat, total Milkfat Moisture

AOAC 963.15 IOCCC 5-1962 AOAC 945.34; 925.41B; 920.80 IOCCC 26-1988 or AOAC 977.10 (Karl Fischer method); or AOAC 931.04 or IOCCC 1-1952

Gravimetry (Soxhlet extraction) Titrimetry/Distillation Gravimetry

I I I

Chocolate and chocolate products Cocoa (Cacao) Mass or Cocoa/ Chocolate Liquor, and Cocoa Cake Cocoa (Cacao) Mass or Cocoa/ Chocolate Liquor, and Cocoa Cake Cocoa butter Cocoa butter

Non-cocoa butter vegetable fat AOCS Ce 10/02 and described in the Standard Cocoa shell Fat Free fatty acids Unsaponifiable matter AOAC 968.10 and 970.23 AOAC 963.15 or IOCCC 14 (1972) ISO660:1996 amended 2003; or AOCS Cd 3d-63 (03) ISO 3596:2000 or ISO 18609: 2000; or AOCS Ca 6b-53 (01) IOCCC 26-1988 or AOAC 977.10 (Karl Fischer method)

Described in the Standard Spiral vessel count, Stone cell count Gravimetry (Soxhlet extraction) Titrimetry Titrimetry after extraction with diethyl ether Gravimetry

I I I I I I

Cocoa powders (cocoa) and dry cocoa- Moisture sugar mixtures Fats and Oils and Related Products Fats and Oils (all) Fats and Oils (all) Fats and Oils (all) Fats and oils Arsenic Arsenic Arsenic Butylhydroxyanisole, butylhydroxytoluene, tertbutylhydroquinone, & propyl gallate Insoluble impurities

AOAC 952.13 (Codex general method) AOAC 942.17 (Codex general method) AOAC 985.16 (Codex general method) AOAC 983.15; or AOCS Ce-6-86 (09)

Colorimetry (diethyldithiocarbamate) Colorimetry (molybdenum blue) Atomic absorption spectrophotometry Liquid chromatography

II III III II

Fats and Oils (all)

Gravimetry ISO 663:2007

Fats and Oils (all)

Lead

AOAC 994.02 ISO 12193:2004 (Codex general method) or AOCS Ca 18c-91 (09) ISO 662:1998 ISO 660:1996; or AOCS Cd 3d-63 (09) AOAC 990.05 ISO 8294:1994 or AOCS Ca 18b-91 (09) (Codex general method) AOCS Cd 8b-90 (09) ISO 3960:2007 ISO 17189 | IDF 194: 2003 ISO 660:2009; or AOCS Cd 3d-63 (09) AOAC 990.05 ISO 8294:1994; or AOCS Ca 18b-91 (09) (Codex general method) ISO 5508 :1990 and ISO 12966-2:2011 or AOCS Ce 2-66 (09) and Ce 1e-91 (09) or Ce 1f-96 (09) ISO 3961: 2009; or AOAC 993.20; or AOCS Cd 1d-1992 (09) AOCS Cd 8b-90 (09) ISO 3960:2007 ISO/AOCS method for apparent density to be inserted ISO 6320:2000 and corr 2006; or AOCS Cc 7-25 (09) ISO 3657:2002; or AOCS Cd 3-25 (09)

Atomic absorption spectrophotometry (direct graphite furnace) Gravimetry (open-drying) Titrimetry Atomic absorption Spectrophotometry (direct graphite furnace) Titrimetry using iso-octane Gravimetry Titrimetry Atomic absorption Spectrophotometry (direct graphite furnace) Gas chromatography of methyl esters Wijs-Titrimetry

II

Fats and Oils (all) Fats and Oils (all)

Matter volatile at 105C Soap content

I I I II

BS 684 Section 2.5; or AOCS Cc 17-95 (09) Gravimetry

Fats and oils not covered by individual Acid Value standards Fats and oils not covered by individual Copper and Iron standards

Fats and oils not covered by individual Peroxide value standards Fat spreads and blended spreads Named Animal Fats Named Animal Fats Fat content Acidity Copper and Iron

I I I II

Named Animal Fats Named Animal Fats

GLC ranges of fatty acid composition Iodine value (IV)

II I

Named Animal Fats Named Animal Fats Named Animal Fats Named Animal Fats

Peroxide value Relative density Refractive index Saponification value

Titrimetry using iso-octane Pycnometry Refractometry Titrimetry

I II II I

Named Animal Fats Named Animal Fats Named Vegetable Oils Named Vegetable Oils Named Vegetable Oils

Unsaponifiable matter Titre Acidity Apparent density

ISO 3596:2000 or ISO 18609: 2000; or AOCS Ca 6b-53 (09) ISO 935:1988; or AOCS Cc 12-59 (09) ISO 660:2009; or AOCS Cd 3d-63 (09) ISO 6883: 2007, with the appropriate conversion factor; or AOCS Cc 10c-95 (09)

Titrimetry after extraction with diethyl ether Thermometry Titrimetry Pycnometry Colour reaction

I I I I I

Named Vegetable Oils Named Vegetable Oils Named Vegetable Oils Named Vegetable Oils Named Vegetable Oils Named Vegetable Oils Named Vegetable Oils

AOCS Cb 2-40 (09) Baudouin test (modified Villavecchia or sesameseed oil test) BS 684 Section 2.20 Carotenoids, total Copper and iron Crismer value GLC ranges of fatty acid composition Halphen test Insoluble impurities Iodine value (IV)

ISO 8294: 1994; or AOAC 990.05; or AOCS Ca 18b-91 (09) AOCS Cb 4-35 (97) and AOCS Ca 5a-40 Turbidity (09) ISO 5508:1990 and ISO 12966-2; or AOCS Gas chromatography of methyl esters Ce 2-66 (09) and Ce 1e-91 (09) or Ce 1f-96 (09) AOCS Cb 1-25 (09) Colorimetry ISO 663: 2000 Gravimetry Wijs - ISO 3961: 1996; or AOAC 993.20; Wijs-Titrimetry 4 or AOCS Cd 1d-1992 (97); or NMKL 39 (2003) AOAC 994.02 ; or ISO 12193: 2004; or AOCS Ca 18c-91 (03) ISO 662: 1998 AOCS Cd 8b-90 (09); or ISO 3960: 2007 Atomic Absorption Gravimetry Titrimetry

Spectrophotometry AAS

II II I II

I I I

Named Vegetable Oils Named Vegetable Oils Named Vegetable Oils

4

Lead Moisture & volatile matter at 105C Peroxide value (PV)

II I I

It is possible to calculate the Iodine Value from fatty acid composition data obtained by gas chromatography e.g. using AOCS Cd 1b-87 (97)

Named Vegetable Oils Named Vegetable Oils Named Vegetable Oils

Refractive index Reichert value and Polenske value Relative density

ISO 6320: 2000 and corr 2006; or AOCS Cc Refractometry 7-25 (09) AOCS Cd 5-40 (09) Titrimetry IUPAC 2.101 with the appropriate conversion factor See comment above (Named Animal Fats) 5 ISO 3657: 2002; or AOCS Cd 3-25 (09) Pycnometry

II I I

Named Vegetable Oils Named Vegetable Oils

Saponification value (SV) Slip point

Titrimetry

I I

ISO 6321:2002 for all oils; Open ended capillary tube AOCS Cc 3b-92 (09) for all oils except palm oils; AOCS Cc 3-25 (09) for palm oils only BS 684 Section 2.5; or AOCS Cc 17-95 (09) Gravimetry ISO 12228: 1999; or AOCS Ch 6-91 (09) ISO 9936:2006 and corrigendum 2008 or AOCS Ce 8-89 (09) ISO 3596:2000; or ISO 18609: 2000; or AOCS Ca 6b-53 (09) COI/T.20/Doc. No. 19 or ISO 3656:2011 or AOCS Ch 5-91 (09). ISO 660:2009 or AOCS Cd 3d-63 (09) ISO 9936:1997 COI/T.20/Doc. no. 20 or AOCS Ce 5b-89 (09) IUPAC 2.4316 ISO 6800:1997 or AOCS Ch 3-91 (09) Gas chromatography HPLC Gravimetry Absorption in ultra violet Titrimetry HPLC Analysis of triglycerides of HPLC and calculation Gas chromatography Gas chromatography

Named Vegetable Oils Named Vegetable Oils Named Vegetable Oils Named Vegetable Oils Olive Oils and Olive Pomace Oils Olive Oils and Olive Pomace Oils Olive Oils and Olive Pomace Oils Olive Oils and Olive Pomace Oils Olive Oils and Olive Pomace Oils Olive Oils and Olive Pomace Oils

Soap content Sterol content Tocopherol content Unsaponifiable matter Absorbency in ultra-violet Acidity, free (acid value) Alpha-tocopherol Difference between the actual and theoretical ECN 42 triglyceride content Erythrodiol + uvaol content Fatty acids in the 2-position of the triglycerides

I II II I II I II I II I

The method is no longer available. The method is no longer available.

10

Olive Oils and Olive Pomace Oils Olive Oils and Olive Pomace Oils Olive Oils and Olive Pomace Oils Olive Oils and Olive Pomace Oils Olive Oils and Olive Pomace Oils Olive Oils and Olive Pomace Oils Olive Oils and Olive Pomace Oils Olive Oils and Olive Pomace Oils Olive Oils and Olive Pomace Oils Olive Oils and Olive Pomace Oils Olive Oils and Olive Pomace Oils Olive Oils and Olive Pomace Oils Olive Oils and Olive Pomace Oils Olive Oils and Olive Pomace Oils Olive Oils and Olive Pomace Oils Olive Oils and Olive Pomace Oils Olive Oils and Olive Pomace Oils Margarine Margarine Margarine Margarine

Halogenated solvents, traces Insoluble impurities in light petroleum Iodine value Iron and copper Lead Moisture and volatile matter Organoleptic characteristics Peroxide value Relative density Refractive index Saponification value Sterol composition and total sterols Stigmastadienes Stigmastadienes Trans fatty acids content Unsaponifiable matter Wax content Fat Milkfat Sodium chloride Vitamin A

COI/T.20/Doc. no. 8 ISO 663:2000 ISO 3961:2009 or AOAC 993.20 or AOCS Cd 1d-92 (09) or NMKL 39 (2003) ISO 8294:1994 or AOAC 990.05 AOAC 994.02 or ISO 12193:2004 or AOCS Ca 18c-91(09) ISO 662:1998 COI/T.20/Doc. no. 15 ISO 3960:2007 or AOCS Cd 8b-90 (09) IUPAC 2.101, with the appropriate conversion factor. See comment above6 ISO 6320:2000 and corr 2006 or AOCS Cc 7-25 (09) ISO 3657:2002 or AOCS Cd 3-25 (09) COI/T.20/Doc. no. 10 or ISO 12228:1999 or AOCS Ch 6-91 (09). COl/T.20/Doc. no. 11 or ISO 157881:1999 or AOCS Cd 26-96 (09). ISO 15788-2: 2003 COI/T.20/Doc no. 17 or ISO 15304:2002 or AOCS Ce 1f-96 (09) ISO 3596:2000 or ISO 18609:2000 or AOCS Ca 6b-53 (09) COI/T.20/Doc. no. 18 or AOCS Ch 8-02 (09) IUPAC 2.801 CAC/RM 15-1969 AOAC 971.27 (Codex general method) AOAC 960.45

Gas chromatography Gravimetry Wijs-Titrimetry AAS AAS Gravimetry Panel test Titrimetry Pycnometry Refractometry Titrimetry Gas chromatography Gas chromatography HPLC Gas chromatography of methyl esters Gravimetry Gas chromatography Gravimetry Titrimetry Potentiometry Spectrophotometry

II I I II II I I I I II I II II III II I II I I II II

11

Margarine Margarine Margarine Minarine Minarine Minarine Minarine Minarine Minarine Minarine Fish and Fishery Products Fish and fishery products Fish and fishery products Fish and fishery products: canned products Fish and fishery products: canned products Boiled Dried Salted Anchovies Canned shrimps or prawns Frozen fish and fishery products

Vitamin D Vitamin E Water Fat Milkfat Sodium chloride Vitamin A Vitamin D Vitamin E Water

AOAC 936.14 IUPAC 2.411 CAC/RM 17-1969 (described in the Standard) IUPAC 2.801 CAC/RM 15-1969 (described in the Standard) AOAC 971.27 (Codex general method) AOAC 960.45 AOAC 936.14 IUPAC 2.411 CAC/RM 17-1969

Bioassay TLC followed by spectrophotometry or GLC Gravimetry Gravimetry Titrimetry Potentiometry Spectrophotometry Bioassay TLC followed by spectrophotometry or GLC Gravimetry

II II I I I II II II II I

Histamine Mercury Drained weight Net weight Sodium Chloride (chloride expressed as sodium chloride) Size, determination of Thawing and cooking procedures

AOAC 977.13 AOAC 977.15 Described in the Standard Described in the Standard AOAC 937.09 Described in the Standard Described in the Standards

Fluorimetry Flameless atomic absorption spectrophotometry Weighing Weighing Titrimetry Number per 100 g Thawing and heating

II III I I II I I

12

Quick frozen blocks of fish fillet, minced fish flesh and mixtures of fillets and minced fish flesh Quick frozen blocks of fish fillet, minced fish flesh and mixtures of fillets and minced fish flesh Quick frozen blocks of fish fillet, minced fish flesh and mixtures of fillets and minced fish flesh Quick frozen fish fillets

Proportion of fish fillet and minced fish Net content of frozen fish blocks covered by glaze Sodium chloride

AOAC 988.09

Physical separation

Described in the Standard

Gravimetry

AOAC 971.21 (Codex general method)

Potentiometry

II

Net weight of products Described in the Standard covered by glaze Quick Frozen Fish sticks (fish fingers) Fish content (declaration) AOAC 996.15 and calculation (described in and fish portions - breaded or in batter the standard) Quick frozen fish sticks (fish fingers) Net weight Described in the Standard and fish portions - breaded or in batter Quick Frozen Fish Sticks (fish fingers) Proportion of fish fillet and WEFTA Method (described in the Stnadard) and Fish Portions-Breaded and in minced fish Batter (except for certain fish species with soft flesh) Quick frozen fish sticks (fish fingers) Sodium chloride AOAC 971.27 (Codex general method) and fish portions - breaded or in batter Salted Atlantic Herring and Salted Water content AOAC 950.46B Sprat Salted Fish of the Gadidae Family Salted Fish and Dried Salted Fish of the Gadidae Family of Fishes Sturgeon Caviar Salt Salt Content Water content Salt content Described in CODEX STAN 167-1989 Sampling and method described in the Standard Described in CODEX STAN 167-1989

Water spraying and sieving Gravimetry Weighing Gravimetry

I I I I

Potentiometry air drying Titrimetry (Mohr) Salt determined as chloride expressed as sodium chloride Gravimetry Titrimetry (Mohr) Salt determined as chloride expressed as sodium chloride

II I I I I

13

Foods for Special Dietary Uses Special foods Special foods Special foods Special foods Special foods Special foods Special foods Special foods Ash Calcium Calories by calculation Carbohydrates Chloride Dietary fibre, total Fat Fat in foods not containing starch, meat or vegetable products Fill of containers Folic acid Linoleate (in the form of glycerides) Linoleate (in the form of glycerides) Loss on drying Loss on drying (milk based) AOAC 942.05 AOAC 984.27 Method described in CAC/VOL IX-Ed.1, Part III Method described in CAC/VOL IX-Ed.1, Part III AOAC 971.27 (Codex general method) AOAC 985.29 CAC/RM 55-1976 CAC/RM 1-1973, B-2 Gravimetry ICP emission spectrometry Calculation method Calculation Potentiometry Gravimetry (enzymatic digestion) Gravimetry (extraction) Gravimetry I III III III II I I I

Special foods Special foods Special foods

CAC/RM 46-1972 AOAC 944.12 AOAC 922.06; 969.33; 963.22

Weighing Microbioassay Acid hydrolysis, preparation of methyl esters and gas chromatography Acid hydrolysis and spectrophotometry Gravimetry Gravimetry

I II II

Special foods Special foods Special foods

AOAC 922.06; 979.19 AOAC 934.01 AOAC 925.23 AOAC 925.23 IDF Standard 21B:1987 ISO 6731:1989 AOAC 961.14

III I I

Special foods

Nicotinamide for foods not based on milk

Colorimetry

II

14

Special foods Special foods Special foods Special foods Special foods Special foods Special foods Special foods Special foods Special foods Special foods

Nicotinamide for milk-based foods Pantothenic acid/enriched foods Pantothenic acid/non-enriched foods Phosphorous Protein efficiency ratio (PER) Protein, crude Riboflavin Sodium and potassium Sodium and potassium Vitamin A

AOAC 944.13 AOAC 945.74 The Analyst 89 (1964):1, 3-6, ibid. 232 US Dept Agr., Agr. Handbook 97 (1965) AOAC 986.24 AOAC 960.48 Method described in CAC/VOL IX-Ed. 1, Part III AOAC 970.65 ISO 8070:1987 (confirmed 1992) IDF Standard 119A:1987 AOAC 984.27 AOAC 974.29

Microbioassay Microbioassay Microbioassay Colorimetry (molybdovanadate) Rat bioassay Titrimetry, Kjeldahl digestion Fluorometry Flame emission spectrophotometry ICP emission spectrometry Colorimetry Spectrophotometry

II II IV II I I II II III IV III

Vitamin A in foods in which AOAC 941.15 carotenes have been added as a source of vitamin A Vitamin B12 Vitamin B6 Vitamin C Vitamin C Vitamin D Vitamin D (D3, milk based infant formula) Vitamin E Vitamin E (milk based infant formula) AOAC 952.20 AOAC 961.15 AOAC 967.22 AOAC 967.21 AOAC 936.14 AOAC 992.26 AOAC 971.30 AOAC 992.03

Special foods Special foods Special foods Special foods Special foods Special foods Special foods Special foods

Microbioassay Microbioassay Microfluorometry Colorimetry (dichloroindophenol) Rat bioassay Liquid chromatography Colorimetry Liquid chromatography

II II II III IV II IV II

15

Follow-up formula follow-up formula Follow-up formula

Dietary fibre, total Iodine (milk based formula) Pantothenic acid

AOAC 991.43 AOAC 992.24 AOAC 992.07 (Measures total pantothenate (free pantothenic acid + CoA- + ACP-bound) and measured as D-pantothenic acid (or calcium D-pantothenate)

Gravimetry (enzymatic digestion) Ion-selective potentiometry Microbioassay

I II II

Follow-up formula Follow-up formula Follow-up formula Follow-up formula

Vitamin A Vitamin A (retinol isomers)

AOAC 974.29 AOAC 992.04

Colorimetry HPLC HPLC HPLC with C30 column to separate the cis- and the trans- K vitamins Titrimetry Gravimetry

IV II III II

Vitamin A (retinol) (above 500 AOAC 992.06 IU/l milk after reconstitution) AOAC 999.15 Vitamin K EN 14148:2003 (vitamin K1) (Measures either aggregated cis + trans K1 or can measure individual cis and trans forms depending on LC column.) Iodine Silica (colloidal, calcium silicate) Biotin Calories (by calculation) AOAC 925.56 AOAC 950.85N

Foods with low-sodium content (including salt substitutes) Foods with low-sodium content (including salt substitutes) Infant formula Infant formula

II IV II I

EN 15607:2008 (d-biotin) HPLC (Measures total D-biotin (free + D-biocytin) Method described in CAC/Vol IX-Ed.1, Calculation Part III7

7 Section 9. Calories by calculation Section 9.2 Conversion Factors (a) protein 4 kcal per g (b) carbohydrate 4 kcal per g (c) fat 9 kcal per g (d) monosaccharides 3.75 kcal per g (e) specific food ingredients See Energy and Protein Requirements(FAO Nutrition Meeting Report Series No. 52 or WHO Technical Report Series No. 522)

16

Infant formula Infant formula Infant formula Infant formula Infant formula

Calcium Calcium Calcium Chloride Choline

ISO 8070 IDF 119: 2007 AOAC 985.35 AOAC 984.27 AOAC 986.26 AOAC 999.14

Flame atomic absorption spectrophotometry Flame atomic absorption spectroscopy ICP emission spectroscopy Potentiometry Enzymatic Colorimetric Method with limitations on applicability due to choline and ascorbate concentration. Flame atomic absorption spectroscopy ICP emission spectroscopy Graphite furnace atomic absorption after dry ashing Graphite furnace AAS after pressure digestion ICP emission spectroscopy Titrimetry (Kjeldahl) Gas chromatography Gas chromatography

II III III III II

Infant formula Infant formula Infant formula Infant formula Infant formula Infant formula Infant formula Infant formula

Copper Copper Chromium (Section B of STAN 72 only) Chromium (Section B of STAN 72 only) Chromium (Section B of STAN 72 only) Crude protein8 Fatty acids (including trans fatty acid) Fatty acids (including trans fatty acid)

AOAC 985.35 AOAC 984.27 EN 14082:2003 EN 14083:2003 AOAC 2006.03 AOAC 991.20 ISO 8968-1/2 | IDF 20-1/2: 2001 AOAC 996.06 AOCS Ce 1h-05 (09)

II III II III III I II III

(f) other specific calorie conversion factors maybe used where the formulation of the food and the nutrient content are known and where such specific conversion factors are physiologically more meaningful than the factors listed above
8

Determination of Crude Protein

The calculation of the protein content of infant formulas prepared ready for consumption may be based on N x 6.25, unless a scientific justification is provided for the use of a different conversion factor for a particular product. The value of 6.38 is generally established as a specific factor appropriate for conversion of nitrogen to protein in other milk products, and the value of 5.71 as a specific factor for conversion of nitrogen to protein in other soy products 17

Infant formula Folic acid

Infant formula

Folic acid

Infant formula

Folic acid

AOAC 992.05 (Measures free folic acid + free, unbound natural folates, aggregated and measured as folic acid) EN 14131:2003 (Total folate (free + bound), aggregated and measured as folic acid) J AOAC Int. 2000:83; 1141-1148 (Measures free folic acid + proportion of free, natural folate) J Chromatogr. A., 928, 77-90, 2001 (Measures total folates after conversion to, and measurement as 5-Me-H4PteGlu) AOAC 992.24 AOAC 985.35 AOAC 984.27 AOAC 999.11NMKL139:1991 ISO 8070 IDF 119: 2007 AOAC 985.35 AOAC 984.27 AOAC 985.35 AOAC 984.27 EN 14083:2003

Microbioassay

II

Optical Biosensor Immunoassay HPLC, incorporating immunoaffinity clean-up and conversion to 5methyltetrahydrofolate Ion-selective potentiometry Flame atomic absorption spectrophotometry ICP emission spectroscopy AAS after dry ashing Flame atomic absorption spectrophotometry Flame atomic absorption spectroscopy ICP emission spectroscopy Flame atomic absorption spectrophotometry ICP emission spectroscopy Graphite furnace AAS after pressure digestion

IV

IV

Infant formula Infant formula Infant formula Infant formula Infant formula Infant formula Infant formula Infant formula Infant formula Infant formula

Iodine (for milk-based formula) Iron9 Iron Iron Magnesium Magnesium Magnesium Manganese Manganese Molybdenum (Section B of STAN 72 only)

II III III II II III III II III II

General Codex methods are also available

18

Infant formula Infant formula Infant formula Infant formula

Melamine Molybdenum (Section B of STAN 72 only) Niacin Niacin

ISO/TS 15495 | IDF/RM 230:2010 AOAC 2006.03 AOAC 985.34 (niacin (preformed) and nicotinamide) prEN 15652:2009 (Free and bound and phosphorylated forms measured either as aggregate of nicotinic acid + nicotinamide, or as individual forms) AOAC 986.24 AOAC 984.27 AOAC 985.31
11

LC-MS/MS ICP emission spectroscopy Microbioassay and turbidimetry HPLC

IV III III II10

Infant formula Infant formula Infant formula Infant formula

Phosphorus Phosphorus Riboflavin Riboflavin

Spectrophotometry (molybdovanadate) ICP emission spectroscopy Fluorimetry HPLC

II III III II

Infant formula

Selenium

EN 14152:2003 (Measures natural and supplemental forms, free, bound and phosphorylated (FMN and FAD) aggregated and measured as riboflavin.) AOAC 996.16 or AOAC 996. 17

Infant formula Infant formula Infant formula Infant formula Infant formula
10 11 12

Selenium Selenium Sodium and potassium Sodium and potassium Thiamine

EN 14627:2005 AOAC 2006.03 AOAC 984.27 ISO 8070 | IDF 119:2007 AOAC 986.2712

Continuous hydride generation Flame atomic absorption spectrometry (HGAAS) Hydride generation atomic absorption spectrometry (HGAAS) ICP emission spectroscopy ICP emission spectrometry Flame atomic absorption spectrophotometry Fluorimetry

III

II III III II III

when published as EN method

Care should be taken in the application of the method due to spectral interference Care should be taken in the application of the method due to spectral interference

19

Infant formula

Thiamine

Infant formula

Total carbohydrates Moisture/Total Solids

EN 14122:2003 (Measures all vitamin B1 forms (natural and added free, bound and phosphorylated) following extraction and conversion to thiamine) AOAC 986.25 AOAC 990.19 or AOAC 990.20 IDF 21B:1987 or ISO 6731:1989 AOAC 942.05 AOAC 989.05 ISO 8381|IDF 123:2008 ISO 8262-1 |IDF 124-1: 2005

HPLC with pre-or post column derivatization to thiochrom

II

Determination by difference Gravimetry

Infant formula Infant formula

Ash Total fat Total fat for milk-based infant formula (Products not completely soluble in ammonia) Total phospholipids

Gravimetry Gravimetry (Rse-Gottlieb) Gravimetry (Weibull-Berntrop)

I I

Infant formula

AOCS Ja7b-91

Infant formula

Vitamin A

Infant formula

Vitamin D

EN 12823-1:2000 (all-trans-retinol and 13cis-retinol) Vitamin A (both natural + supplemental ester forms) aggregated and quantified as individual retinol isomers (13 - cis and alltrans) AOAC 992.26 D3 measured

Gas chromatography with suitable extraction and preparation procedures HPLC

III

III

HPLC

III

20

Infant formula

Vitamin D

Infant formula Infant formula

Vitamin D Vitamin E

Infant formula

Vitamin E

Infant formula Infant formula

Vitamin B6 Vitamin B6

EN 12821:2000 (D2 and/or D3 measured as single components. Hydroxylated forms not measured.) NMKL 167: 2000 AOAC 995.05 D2 and D3 measured AOAC 992.03 Measures all rac-vitamin E (both natural + supplemental ester forms) aggregated and quantified as -congeners EN 12822: 2000 (Measures Vitamin E (both natural + supplemental ester forms) aggregated and quantified as individual tocopherol congeners (, , , ). AOAC 985.32 EN 14166:2008 (Aggregates free and bound pyridoxal, pyridoxine and pyridoxamine and measures as pyridoxine) AOAC 2004.07 EN 14164:2008 (Free and bound phosphorylated forms (pyridoxal, pyridoxine and pyridoxamine) converted and measured as pyridoxine) EN 14663:2005 (includes glycosylated forms) (Free and bound phosphorylated and glycosylated forms measured as the individual forms pyridoxal, pyridoxine and pyridoxamine)

HPLC

II

HPLC HPLC

III III

HPLC

II

Microbioassay Microbioassay

III III

Infant formula

Vitamin B6

II HPLC

Infant formula

Vitamin B6

HPLC

III

21

Infant formula Vitamin B12 Infant formula Vitamin C

Infant formula Infant formula

Zinc Zinc

AOAC 986.23 (Measures total vitamin B12 as cyanocobalamin) EN 14130:2003 (Measures ascorbic acid + dehydroascorbic acid) AOAC 985.35 AOAC 984.27

Turbidimetric Method

II

HPLC

II

Flame atomic absorption spectroscopy ICP emission spectroscopy

II III

Methods of analysis for dietary fibre: Guidelines for Use of Nutrition and Health Claims: Table of Conditions for Claims Standard Provisions Method General methods that do not measure the lower molecular weight fraction (i.e. monomeric units<=9)(2) All foods Method applicable for determining dietary fibres that do not include AOAC 985.29 (1) the lower molecular weight fraction. (4) AACC Intl 32-05.01 (1991,1999) All foods Method applicable for determining dietary fibres that do not include AOAC 991.43 (1) the lower molecular weight fraction and also includes determination AACC Intl 32-07.01 (1999, 1991) for soluble and insoluble dietary fibres (4) NMKL 129, 2003 All foods Method applicable for determining dietary fibres that do not include AOAC 993.21 (1) the lower molecular weight fraction, in foods and food products containing more than 10% dietary fibres and less than 2% starch (e.g. fruits) (4) All foods Method applicable for determining dietary fibres that do not include AOAC 994.13 (1) the lower molecular weight fraction. Provides sugar residue AACC Intl 32- 25.01 (1999, 1994) composition of dietary fibre polysaccharides, as well as content of NMKL 162, 1998 Klason lignin (4). All foods Insoluble dietary fibres in food and food products (4) (1) All foods Soluble dietary fibres in food and food products (4) (1) AOAC 991.42 (Specific for insoluble fibre) AACC Intl 32-20.01 (1999, 1982) AOAC 993.19 (Specific for soluble fibre) Principle Enzymatic gravimetry Enzymatic gravimetry gravimetry Type Type I Type I Type I

Enzymatic colorimetry gravimetry Enzymatic gravimetry Enzymatic gravimetry

GC/ Type I

Type I Type I

22

General methods that measure both the higher (monomeric units > 9) and the lower molecular weight fraction (monomeric units <=9) (2) All foods AOAC 2001.03 Enzymatic Type I (1) Method applicable for determining the content of dietary fibres of AACC Intl 32-41.01 (2002) gravimetry and higher and lower molecular weight, in food where resistant starches Liquid are not present chromatography Type I All foods Method applicable for determining the content of dietary fibres of AOAC 2009.01 Enzymatic(1) higher and lower molecular weight. The method is applicable in AACC Intl 32-45.01 (2009) Gravimetry food that may, or may not, contain resistant starches. High Pressure Liquid Chromatography Methods that measure individual specific components (monomeric units: the whole range for each type of components is covered)(2) All foods (13)(14) Beta-D-Glucans AOAC 995.16 Enzymatic Type II (1) AACC Intl 32-23.01 (1999, 1995) All foods Fructans (oligofructoses, inulin, hydrolyzed inulin, polyfructoses, AOAC 997.08 Enzymatic & Type II (1) fructooligosaccharides) AACC Intl 32-31.01 (2001) HPAEC-PAD (applicable to added fructans) All foods Fructans (oligofructoses, inulin, hydrolyzed inulin, polyfructoses, AOAC 999.03 Enzymatic & Type III (1) fructooligosaccharides) AACC Intl 32-32.01 (2001) colorimetric (not applicable highly depolymerised fructans) All foods Polydextrose AOAC 2000.11 HPAEC-PAD Type II (1) AACC Intl 32-28.01 (2001) All foods Trans-galacto-oligo saccharides AOAC 2001.02 HPAEC-PAD Type II (1) AACC Intl 32-33.01 (2001) All foods Resistant starch (Recommended for RS3) AOAC 2002.02 Enzymatic Type II (1) AACC Intl 32-40.01 (2002)

23

Other methods(2) that have not been subjected to interlaboratory evaluation under AOAC international guidelines All foods Insoluble glucans and mannans of yeast cell wall (for yeast cell Eurasyp (European association for wall only) specialty yeast product) LM Bonanno. Biospringer- 2004 online version : http://www.eurasyp.org/public.techniq ue.home.screen. All foods Fructo-oligosaccharides (monomeric units<5) Ouarn et al. 1999 in Complex Carbohydrates in Foods. Edited by S. Sungsoo, L. Prosky & M. Dreher. Marcel Dekker Inc, New York All foods Non-starch polysaccharides (NSP) (3) Englyst H.N, Quigley M.E., Hudson G. (1994) Determination of dietary fibre as non-starch polysaccharides with gas-liquid chromatographic high performance liquid chromatographic or spectrophotometric measurement of constituent sugars Analyst 119, 1497-1509
(1)

Chemical & Type IV HPAEC-PAD

HPAEC-PAD

Type IV

Gas-Liquid Chromatography

Type IV

Users should consult the description of each method for the food matrices that were the subject of interlaboratory study in the Official methods of Analysis of AOAC International.

Two issues are left for national authorities: to include monomeric units 3-9 and which isolated or synthetic compounds have physiological benefit. (Refer to the Guidelines for Nutrition Labelling (CAC/GL 2-1985), as revised in 2009.
(3) (4)

(2)

Quantitation lost for resistant starch. Refer to specific methods. Quantitation lost for inulin, resistant starch, polydextrose and resistant maltodextrins. Refer to specific methods.

24

Fruit Juices and Nectars Commodity Fruit Juices and Nectars Fruit Juices and Nectars Fruit Juices and Nectars Fruit Juices and Nectars Fruit Juices and Nectars Fruit Juices and Nectars Fruit Juices and Nectars Fruit Juices and Nectars Fruit Juices and Nectars Fruit Juices and Nectars Fruit Juices and Nectars Fruit Juices and Nectars Fruit Juices and Nectars Fruit Juices and Nectars
13

Provisions Ascorbic acid-L (additives) Ascorbic acid-L (additives) Ascorbic acid-L (additives) Carbon dioxide (additives and processing aids) Cellobiose Citric acid 13(additives) Citric acid 5(additives) Glucose and fructose (permitted ingredients) Glucose-D and fructose-D (permitted ingredients) HFCS & HIS in apple juice (permitted ingredients) Malic acid (additives) Malic acid-D Malic acid-D in apple juice Malic acid-L

Method IFU Method No 17a (1995) ISO 6557-1: 1986 AOAC 967.21 IFU Method No 17 ISO 6557-2: 1984 IFU Method No 42 (1976) IFU Recommendation No.4 October 2000 AOAC 986.13 EN 1137: 1994 IFU Method No 22 (1985) EN 12630 IFU Method No 67 (1996) NMKL 148 (1993) EN 1140 IFU Method No 55 (1985) Determination of HFCS & HIS by Capillary GC method JAOAC 84, 486 (2001) AOAC 993.05 EN 12138 IFU Method No 64 (1995) AOAC 995.06 EN 1138 (1994) IFU Method No 21 (1985)

Principle HPLC Fluorescence spectrometry Indophenol method Titrimetry (back-titration after precipitation) Capillary gas chromatography HPLC Enzymatic determination HPLC Enzymatic determination CAP GC Method Enzymatic determination and HPLC Enzymatic determination HPLC Enzymatic determination

Type II IV III IV IV II III III II IV III II II II

All juices except citrus based juices

25

Fruit Juices and Nectars Fruit Juices and Nectars Fruit Juices and Nectars Fruit Juices and Nectars Fruit Juices and Nectars

Pectin (additives) Benzoic acid and its salts; sorbic acid and its salts Benzoic acid and its salts Preservatives in fruit juices (sorbic acid and its salts) Quinic, malic & citric acid in cranberry juice cocktail and apple juice (permitted ingredients and additives) Saccharin Soluble solids

IFU Method No 26 (1964/1996) IFU Method No 63 (1995) NMKL 124 (1997) ISO 5518:1978 ISO 6560: 1983 ISO 5519: 1978 Determination of quinic, malic and citric acid in cranberry juice cocktail and apple juice AOAC 986.13 NMKL 122 (1997) AOAC 983.17 EN 12143 (1996) IFU Method No 8 (1991) ISO 2173: 2003 EN 12146 (1996) IFU Method No 56 (1985/1998) EN 12630 IFU Method No 67 (1996) NMKL 148 (1993) Optimized Monier Williams AOAC 990.28 IFU method No. 7A (2000) NMKL 132 (1989) NMKL 135 (1990) ISO 5522:1981 ISO 5523:1981 EN 12137 (1997) IFU Method No 65 (1995) EN 12135 (1997) IFU Method No 28 (1991)

Precipitation/photometry HPLC Spectrometry Spectrometry HPLC

I II III III III

Fruit Juices and Nectars Fruit Juices and Nectars

Liquid chromatography Indirect by refractometry

II I

Fruit Juices and Nectars Fruit Juices and Nectars Fruit Juices and Nectars Fruit Juices and Nectars Fruit Juices and Nectars Fruit Juices and Nectars Fruit Juices and Nectars

Sucrose (permitted ingredients) Sucrose (permitted ingredients) Sulphur dioxide (additives) Sulphur dioxide (additives) Sulphur dioxide (additives) Tartaric acid in grape juice (additives) Total nitrogen

Enzymatic determination HPLC Titrimetry after distillation Enzymatic determination Titrimetry after distillation HPLC Digestion/titration

III II II III III II I

26

Fruit Juices and Nectars Fruit Juices and Nectars Fruit Juices and Nectars Fruit Juices and Nectars Fruit Juices and Nectars Fruit Juices and Nectars Fruit Juices and Nectars Fruit Juices and Nectars Fruit Juices and Nectars Fruit Juices and Nectars

Sections 3.2 Quality Criteria and 3.3 Authenticity14

Determination of acetic acid EN 12632; IFU Method No 66 (1996) Determination of alcohol (ethanol) IFU Method No 52 (1996) Detection of anthocyanins IFU Method No 71 (1998) Determination of ash in fruit products AOAC 940.26 ;EN 1135 (1994); IFU Method No 9 (1989) Detection of beet sugar in fruit juices AOAC 995.17 Determination of benzoic acid as a marker in orange juice AOAC 994.11 Determination of C13/C12 ratio of ethanol derived from fruit juices JAOAC 79, No. 1, 1996, 62-72 Determination of carbon stable isotope ratio of apple juice AOAC 981.09 - JAOAC 64, 85 (1981) Determination of carbon stable isotope ratio of orange juice AOAC 982.21 Determination of carotenoid, total/individual groups EN 12136 (1997); IFU Method No 59 (1991)

Enzymatic determination Enzymatic determination HPLC Gravimetry Deuterium NMR HPLC Stable isotope mass spectrometry Stable isotope mass spectrometry Stable isotope mass spectrometry Spectrophotometry

II II I I II III II II II I

3.4 Verification of Composition, Quality and Authenticity Fruit juices and nectars should be subject to testing for authenticity, composition, and quality where applicable and where required. The analytical methods used should be those found in Section 9, Methods of Analysis and Sampling. The verification of a samples authenticity / quality can be assessed by comparison of data for the sample, generated using appropriate methods included in the standard, with that produced for fruit of the same type and from the same region, allowing for natural variations, seasonal changes and for variations occurring due to processing.

14

27

Fruit Juices and Nectars Fruit Juices and Nectars

Fruit Juices and Nectars Fruit Juices and Nectars Fruit Juices and Nectars Fruit Juices and Nectars Fruit Juices and Nectars Fruit Juices and Nectars Fruit Juices and Nectars Fruit Juices and Nectars

Fruit Juices and Nectars Fruit Juices and Nectars Fruit Juices and Nectars

Determination of centrifugable pulp EN 12134 (1997) - IFU Method No 60 (1991) Determination of chloride (expressed as sodium chloride) EN12133 (1997) IFU Method No 37 (1991) Determination of chloride in vegetable juice AOAC 971.27 (Codex general method) ISO 3634:1979 Determination of essential oils (Scott titration AOAC 968.20 - IFU 45b* Determination of essential oils (in citrus fruit) (volume determination)* ISO 1955:1982 Determination of fermentability IFU Method No 18 (1974) Determination of formol number EN 1133 (1994) IFU Method No 30 (1984) Determination of free amino acids EN 12742 (xxxx) IFU Method No 57 (1989) Determination of fumaric acid IFU Method No 72 (1998) Determination of glucose fructose and saccharose EN 12630 - IFU Method No 67 (1996) NMKL 148 (1993) Determination of gluconic acid IFU Method No 76 (2001) Determination of glycerol IFU Method No 77 (2001) Determination of hesperidin and naringin EN 12148 (1996) - IFU Method No 58 (1991)

Centrifugation/% value Electrochemical titrimetry

I III

Titration (Scott) distillation, titration Distillation and direct reading of the volume determination Microbiological method Potentiometric titration Liquid Chromatography HPLC HPLC

II I I I I II II II

Enzymatic determination Enzymatic determination HPLC

II II II

28

Fruit Juices and Nectars Fruit Juices and Nectars Fruit Juices and Nectars Fruit Juices and Nectars Fruit Juices and Nectars Fruit Juices and Nectars Fruit Juices and Nectars

Fruit Juices and Nectars Fruit Juices and Nectars Fruit Juices and Nectars Fruit Juices and Nectars Fruit Juices and Nectars Fruit Juices and Nectars

Determination of hydroxymethylfurfural IFU Method No 69 (1996) Determination of hydroxymethylfurfural ISO 7466:1986 Determination of isocitric acid-D IFU Method No 54 (1984) Determination of Lactic acid- D and L EN 12631 (1999) IFU Method No 53 (1983/1996) Determination of L-malic/total malic acid ratio in apple juice AOAC 993.05 Determination of naringin and neohesperidin in orange juice AOAC 999.05 Determination of pH-value NMKL 179:2005 EN 1132 (1994);IFU Method No 11 (1989);ISO 1842: 1991 Determination of phosphorus/phosphate EN 1136 (1994) IFU Method No 50 (1983) Determination of proline by photometry non-specific determination EN 1141 (1994); IFU Method No 49 (1983) Determination of relative density EN 1131 (1993); IFU Method No 1 (1989) & IFU Method No General sheet (1971) Determination of Relative density IFU Method No 1A Determination of sodium, potassium, calcium, magnesium in fruit juices EN 1134 (1994); IFU Method No 33 (1984) Determination of sorbitol-D IFU Method No 62 (1995)

HPLC Spectrometry Enzymatic determination Enzymatic determination Enzymatic determination and HPLC HPLC Potentiometry

II III II II II III

II Photometric determination Photometry Pycnometry Densitometry Atomic Absorption Spectroscopy Enzymatic determination IV II I II III II II

29

Fruit Juices and Nectars

Fruit Juices and Nectars

Fruit Juices and Nectars Fruit Juices and Nectars Fruit Juices and Nectars Fruit Juices and Nectars

Fruit Juices and Nectars

Fruit Juices and Nectars

Fruit Juices and Nectars Fruit Juices and Nectars Fruit Juices and Nectars

Determination of stable carbon isotope ratio in the pulp of fruit juices ENV 13070 (1998) Analytica Chimica Acta 340 (1997) Determination of stable carbon isotope ratio of sugars from fruit juices ENV 12140 Analytica Chimica Acta.271 (1993) Determination of stable hydrogen isotope ratio of water from fruit juices ENV 12142 (1997) Determination of stable oxygen isotope ratio in fruit juice water ENV 12141(1997) Detection of starch AOAC 925.38 (1925) IFU Method No 73 (2000) Determination of sugar beet derived syrups in frozen concentrated orange juice 18O Measurements in Water AOAC 992.09 Determination of titrable acids, total EN 12147 (1995) IFU Method No Method No 3, (1968) ISO 750:1998 Determination of total dry matter (vacuumoven drying at 70C)* EN 12145 (1996) IFU Method No 61 (1991) Determination of total solids (Microwave oven drying)* AOAC 985.26 Determination of Vitamin C (dehydroascorbic acid and ascorbic acid) AOAC 967.22 Determination of Vitamin C EN 14130 : 2004

Stable isotope mass spectrometry

II

Stable isotope mass spectrometry

II

Stable isotope mass spectrometry Stable isotope mass spectrometry Colorimetric Oxygen isotope ratio analysis

II II I I

Titrimetry

Gravimetric determination

Gravimetric determination Microfluorometry HPLC

I III II

30

Because there is no numerical value in the Standard duplicate Type I methods have been included which may lead to different results.

Milk and Milk Products Milk products Iron NMKL 139 (1991) AOAC 999.11 (Codex general method) NMKL 161 (1998) / AOAC 999.10 AOAC 984.27 ISO 6732| IDF 103:2010 ISO/TS 15495 | IDF/RM 230:2010 IDF 124-3 | ISO 8262-3:2005 ISO 1737| IDF 13:2008 ISO 6731|IDF 21:2010 and ISO 1737| IDF 13:2008 ISO 8968-1/2|IDF 20-1/2:2001/ AOAC 991.20 ISO 1737| IDF 13:2008 Atomic absorption spectrophotometry Atomic spectrophotometry absorption II

Milk products Milk products Milk products Milk and Milk Products Milk products (products not completely soluble in ammonia) Blend of evaporated skimmed milk and vegetable fat Blend of evaporated skimmed milk and vegetable fat Blend of evaporated skimmed milk and vegetable fat Reduced fat blend of evaporated skimmed milk and vegetable fat

Iron Iron Iron Melamine Milkfat Total fat Milk solids-not-fat (MSNF)

III III IV IV I IV I I IV IV

Inductively Coupled Plasma optical emission spectrophotometry Photometry (bathophenanthroline) LC-MS/MS Gravimetry (Weibull-Berntrop) Gravimetry (Rse-Gottlieb) Calculation from total solids content and fat content Gravimetry (Rse-Gottlieb) Titrimetry (Kjeldahl) Gravimetry (Rse-Gottlieb)

Milk protein in MSNF15 Total fat

15

Milk total solids and Milk solids-not-fat content include water of crystallization of lactose Milk total solids and MSNF content include water of crystallization of lactose

31

Reduced fat blend of evaporated skimmed milk and vegetable fat Reduced fat blend of Evaporated skimmed milk and vegetable fat Blend of skimmed milk and vegetable fat in powdered form Blend of skimmed milk and vegetable fat in powdered form Blend of skimmed milk and vegetable fat in powdered form Reduced fat blend of skimmed milk powder and vegetable fat in powdered form Reduced fat blend of skimmed milk powder and vegetable fat in powdered form Reduced fat blend of skimmed milk powder and vegetable fat in powdered form Blend of sweetened condensed skimmed milk and vegetable fat Blend of sweetened condensed skimmed milk and vegetable fat Blend of sweetened condensed skimmed milk and vegetable fat Blend of sweetened condensed skimmed milk and vegetable fat

MSNF15 Milk protein in MSNF15 Total fat Water16 Milk protein in MSNF15 Total fat WaterError! Bookmark not defined. Milk protein in MSNF15 Total fat Sucrose Milk solids-not-fat (MSNF)15 Milk protein in MSNF15

ISO 6731|IDF 21:2010 and ISO 1737| IDF 13:2008 ISO 8968-1/2|IDF 20-1/2:2001/ AOAC 991.20 ISO 1736|IDF 9:2008 ISO 5537|IDF 26:2004 ISO 8968-1/2|IDF 20-1/2:2001/ AOAC 991.20 ISO 1736|IDF 9:2008 ISO 5537|IDF 26:2004 ISO 8968-1/2|IDF 20-1/2:2001/ AOAC 991.20 ISO 1737| IDF 13:2008 ISO 2911|IDF 35:2004 ISO 6734|IDF 15:2010 ISO 8968-1/2|IDF 20-1/2:2001/ AOAC 991.20

Calculation from total solids content and fat content Gravimetry (Rse-Gottlieb) Titrimetry (Kjeldahl) Gravimetry (Rse-Gottlieb) Gravimetry, drying at 87 C Titrimetry (Kjeldahl) Gravimetry (Rse-Gottlieb) Gravimetry, drying at 87 C Titrimetry (Kjeldahl) Gravimetry (Rse-Gottlieb) Polarimetry Calculation from total solids content Titrimetry (Kjeldahl)

I IV I I IV I I IV I IV IV IV

16

Water content excluding the crystallized water bound to lactose (generally known as moisture content)

32

Reduced fat blend of sweetened condensed skimmed milk and vegetable fat Reduced fat blend of sweetened condensed skimmed milk and vegetable fat Reduced fat blend of sweetened condensed skimmed milk and vegetable fat Butter Butter Butter Butter Butter Butter

Total fat MSNF15 Milk protein in MSNF15 Copper Lead Milk solids-not-fat Milkfat Milk fat purity Salt

ISO 1737| IDF 13:2008 ISO 6734|IDF 15:2010 ISO 8968-1/2| IDF 20-1/2:2001/ AOAC 991.20 ISO 5738|IDF 76:2004 AOAC 960.40 AOAC 972.25 (Codex general method) IDF 80-2 | ISO 3727-2:2001 IDF 80-3 | ISO 3727-3:2003 ISO 17678|IDF 202:2010 ISO 1738|IDF 12:2004 / AOAC 960.29 IDF 179 | ISO 15648:2004

Gravimetry (Rse-Gottlieb) Calculation from total solids content Titrimetry (Kjeldahl) Photometry, diethyldithiocarbamate Atomic absorption spectrophotometry Gravimetry Gravimetry Calculation from determination of triglycerides by gas chromatography Titrimetry (Mohr: determination of chloride, expressed as sodium chloride) Potentiometry (determination of chloride, expressed as sodium chloride) Gas chromatography Gas chromatography Gravimetry Enzymatic method Photometry Gravimetry (Schmid-BondzynskiRatslaff)

I IV IV II II I I I II

Butter

Salt

III

Butter Butter Butter Cheese Cheese Cheese

Vegetable fat (sterols) Vegetable fat (sterols) Water Citric acid Citric acid Milkfat

ISO 12078|IDF 159:2006 ISO 18252|IDF 200:2006 IDF 80 | ISO 37271:2001 ISO/TS 2963|IDF/RM 34:2006 AOAC 976.15 ISO 1735|IDF 5:2004

II III I IV II I

33

Cheese Cheese (and cheese rind) Cheese

Moisture Natamycin Sodium chloride

ISO 5534|IDF 4:2004 ISO 9233-1|IDF 140-1:2007 ISO 5943|IDF 88:2006 ISO 5534/IDF 4: 2004 IDF 5 | ISO 1735:2004

Gravimetry, drying at 102 C Molecular absorption spectrophotometry Potentiometry (determination of chloride, expressed as sodium chloride) Gravimetry, drying at 102C Gravimetry (Schmid-BondzynskiRatzlaff) Calculation from dry matter content and fat content Gravimetry, drying at 102 C Gravimetry (Schmid-BondzynskiRatzlaff) Gravimetry (Schmid-BondzynskiRatzlaff) (for samples containing lactose up to 5%) Gravimetry (Weibull-Berntrop) (for samples containing lactose over 5%) Gravimetry (Weibull-Berntrop) Titrimetry, Kjeldahl Titrimetry (Kjeldahl) Gravimetry (Rse-Gottlieb) Gravimetry (drying at 102C) Gravimetry Gravimetry

I III II

Cheeses, individual Cheeses in brine Cottage cheese

Dry matter (Total solids) Milkfat in dry matter (FDM) Fat-free dry matter

I I I

ISO 5534|IDF 4:2004 and ISO 1735|IDF 5:2004 Cottage cheese Milk fat ISO 1735|IDF 5:2004 ISO 8262-3|IDF 124-3:2005 Cottage cheese Milk fat in dry matter ISO 8262-3|IDF 124-3:2005 ISO 8968-1/2|IDF 20-1/2:2001/ AOAC 991.20 and 991.23 ISO 8968-1/2|IDF 20-1/2:2001/ AOAC 991.20 ISO 2450|IDF 16:2008 ISO 6731|IDF 21:2010 ISO 2450|IDF 16:2008 / AOAC 995.19 ISO 3727-2|IDF 80-2:2001 AOAC 920.116

I I I I I I I I I

Cheese, Unripened Including Fresh Protein Cheese Cream and Prepared Creams Cream Cream Creams Lowered in Milkfat Content Creams, Whipped Fermented Creams Creams Milk protein Milkfat Solids Milkfat

and Milk solids-not-fat

34

Cream cheese Cream cheese

Dry matter Moisture on fat free basis

ISO 5534|IDF 4:2004

Gravimetry drying at 102 C (forced air oven) Calculation from fat content and moisture content Gravimetry drying at 102C (forced air oven) Gravimetry (Schmid-BondzynskiRatzlaff) Calculation from determination of triglycerides by gas chromatography Gravimetry Direct determination of fat using solvent extraction Gas chromatography Gas chromatography

ISO 5534|IDF 4:2004 ISO 1735|IDF 5:2004 Dairy fat spreads Dairy fat spreads Dairy fat spreads Dairy fat spreads Milk fat purity Total fat Vegetable fat (sterols) Vegetable fat (sterols) ISO 17678|IDF 202:2010 ISO 17189|IDF 194:2003 ISO 12078|IDF 159:2006 ISO 18252|IDF 200:2006

I I II III IV

Edible casein products Edible casein products Edible Casein Products Edible casein products Edible casein products Edible casein products

Acids, free Ash (including P2O5) Casein in protein Copper Copper Lactose

ISO 5547|IDF 91:2008 ISO 5545|IDF 90:2008 or17 ISO 5544|IDF 89:2008 ISO 17997-1|IDF 29-1:2004 AOAC 985.35 IDF 76 | ISO 5738:2004 IDF 106 | ISO 5548:2004

Titrimetry (aqueous extract) Gravimetry (ashing at 825 C ) Titrimetry, Kjeldahl Atomic absorption spectrophotometry Colorimetry (diethyldiethiocarbamate) Photometry (phenol and H2SO4)

I I II III IV

17

refer to scope of methods

35

Edible casein products

Lead

NMKL 139 (1991) (Codex general method) AOAC 999.11 NMKL 161 (1998) / AOAC 999.10 AOAC 972.25 (Codex general method) AOAC 982.23 (Codex general method) IDF RM 133 | ISO TS 6733: 2006 ISO 5543 | IDF 127: 2004 ISO 5550|IDF 78:2006 ISO 5546|IDF 115:2010 IDF 92:1979 / ISO 5549:1978 ISO 5739|IDF 107:2003 ISO 5550|IDF 78:2006 ISO 8070|IDF 119:2007 ISO 1737| IDF 13:2008
18

Atomic absorption spectrophotometry

II

Edible casein products Edible casein products Edible casein products Edible casein products Edible casein products Edible casein products Edible casein products Edible casein products Edible casein products Edible casein products Emmental Evaporated milks Evaporated milks Evaporated milks Fermented milks Fermented milks

Lead Lead Lead Lead Milkfat Moisture pH Protein (total N x 6.38 in dry matter) Sediment (scorched particles) WaterError! Bookmark not defined. Calcium >= 800mg/100g Milk fat Protein

Atomic absorption spectrophotometry Atomic absorption spectrophotometry Anodic stripping voltanmetry Spectrophotometry (1,5diphenylthiocarbazone) Gravimetry (Schmid-BondzynskiRatslaff) Gravimetry (drying at 102C) Electrometry Titrimetry, Kjeldahl digestion Visual comparison with standard disks, after filtration Gravimetry (drying at 102 C) Flame atomic absorption Gravimetry (Rse-Gottlieb) Titrimetry (Kjeldahl) Gravimetry (drying at 102C) Colony-count at 25 C Gravimetry (drying at 102 C)

III III III III I I IV IV IV I IV I I I IV I

ISO 8968-1/2|IDF 20-1/2:2001 AOAC 945.48H / AOAC 991.20 Solids, total ISO 6731|IDF 21:2010 Colony-forming units of yeasts ISO 6611|IDF 94:2004 and/or moulds Dry matter (total solids) ISO 13580|IDF 151:2005

36

Fermented milks Fermented milks Fermented milks Yoghurt and yoghurt products Fermented milks Yoghurt and yoghurt products Fermented milks Fermented milks Fermented milks Milk powders and cream powders Milk powders and cream powders Milk powders and cream powders Milk powders and cream powders Milk powders and cream powders Milk powders and cream powders Milk fat products Milk fat Products Milk fat products Milk fat products

Lactic acid (total acidity expressed as lactic acid) Lactobacillus acidophilus Lactobacillus delbrueckii subsp bulgaricus & Streptococcus thermophilus Lactobacillus delbrueckii subsp bulgaricus & Streptococcus thermophilus Microorganisms constituting the starter culture Milk fat Protein Acidity, titratable Milk fat Protein (in MSNF15) Scorched particles Solubility Index WaterError! Bookmark not defined. Antioxidants (phenolic) Copper Fatty acids, free (expressed as oleic acid) Milk fat

IDF 150:1991 / ISO 11869:1997 ISO 20128|IDF 192:2006 ISO 7889|IDF 117:2003 ISO 9232|IDF 146:2003 ISO 27205|IDF 149:2010 (Annex A)

Potentiometry, titration to pH 8.30 Spectrophotometry Colony count at 37 C Colony count at 37C Test for strain identification Colony count at 25 C, 30 C, 37 C and 45 C according to the starter organism in question Gravimetry (Rse-Gottlieb)

IV I I I

IV I I I I I IV I I II II I IV

ISO 1211|IDF 1:2010 / AOAC 989.05

ISO 8968-1/2|IDF 20-1/2:2001/ Titrimetry (Kjeldahl) AOAC 991.20 ISO 6091|IDF 86:2010 Titrimetry, titration to pH 8.4 ISO 1736|IDF 9:2008 Gravimetry (Rse-Gottlieb) ISO 8968-1/2|IDF 20-1/2:2001 / AOAC Titrimetry (Kjeldahl digestion) 991.20 ISO 5739|IDF 107:2003 Visual comparison with standard disks, after filtration ISO 8156|IDF 129:2005 Centrifugation ISO 5537|IDF 26:200419 Gravimetry (drying at 87C) IDF 165:1993 ISO 5738|IDF 76:2004 AOAC 960.40 ISO 1740|IDF 6:2004 IDF 24:1964 Reversed phase gradient liquid chromatography Photometry, diethyldithiocarbamate Titrimetry Gravimetry (calculation from solidsnot-fat and water content)

19

The method has only been validated for milk powders, not for cream powders

37

Milk fat products Milk fat Products Milk fat products

Milk fat purity Peroxide value (expressed as meq. of oxygen/kg fat) Vegetable fat (sterols)

ISO 17678|IDF 202:2010 ISO 3976|IDF 74:2006 ISO 12078|IDF 159:2006 ISO 18252|IDF 200:2006

Calculation from determination of triglycerides by gas chromatography Photometry Gas chromatography Gas chromatography Titrimetry (Karl Fischer) Photometry Titrimetry Titrimetry (Kjeldahl) Gravimetry after solvent extraction Gravimetry after solvent extraction Enzymatic method Photometry Gravimetry (Schmid- BondzynskiRatzlaff) Calculation from phosphorous and nitrogen content Spectrophotometry (molybdateascorbic acid) Potentionmetry (determination of chloride, expressed as sodium chloride)

I I II III II I I I I I IV III I IV II II

Milk fat products Milk fat products (anhydrous milk fat) Milkfat products (anhydrous milkfat) Milk Products obtained from Fermented Milks Heat-Treated after Fermentation Mozzarella Mozzarella Processed cheese products Processed cheese products Processed cheese products Processed cheese products Processed cheese products Processed cheese products

Water Peroxide value Peroxide value Protein Milkfat in dry matter with high moisture Milkfat in dry matter with low moisture Citric acid Citric acid Milkfat Phosphate, added (expressed as phosphorus) Phosphorus Salt

ISO 5536|IDF 23:2009 ISO 3976|IDF 74:2006 AOAC 965.33 ISO 8968-1/2|IDF 20-1/2:2001 / AOAC 991.20 ISO 1735|IDF 5:2004 ISO 1735|IDF 5:2004 ISO/TS 2963|IDF/RM 34:2006 AOAC 976.15 ISO 1735|IDF 5:2004 IDF 51B:1991 IDF 33C:1987 / ISO 2962:1984 ISO 5943|IDF 88:2004

38

Sweetened condensed milk Sweetened Condensed Milks Sweetened Condensed Milks Whey Cheese Whey cheeses by concentration Whey cheeses by coagulation Whey cheese

Milkfat Protein Solids Dry matter (for denomination) Dry matter (total solids) Dry matter (total solids) Fat on the dry basis

ISO 1737:1999 ISO 1737| IDF 13:2008 Gravimetry (Rse-Gottlieb) ISO 8968-1/2|IDF 20-1/2:2001 / AOAC Titrimetry (Kjeldahl) 945.48H / AOAC 991.20 ISO 6734|IDF 15:2010 Gravimetry, drying at 102 C IDF 58 | ISO 2920:2004 IDF 58 | ISO 2920:2004 IDF 4:2004 ISO 5534:2004 IDF 59 A:1986 | ISO 1854:1999 and IDF 58:2004 | ISO 2920:2004 ISO 1735|IDF 5:2004 ISO 1735|IDF 5: 2004 and ISO 5534|IDF 4:2004 Gravimetry, drying at 88 C Gravimetry, drying at 88 C Gravimetry, Drying at 102C Calculation from fat content and dry matter content

I I I I I IV I

Whey cheeses by coagulation Whey cheeses by coagulation

Milk fat Milk fat in dry matter

Gravimetry Ratzlaff)

(Schmid-Bondzynski-

I I

Calculation from fat content and dry matter content Gravimetry (Schmid-BondzynskiRatzlaff) Gravimetry, drying at 102C Gravimetry (Rse Gottlieb)

Whey cheeses by concentration Whey cheeses by concentration

Milk fat Milk fat in dry matter

ISO 1854|IDF 59:2008 ISO 1854|IDF 59:2008 and ISO 2920|IDF 58:2004 IDF 59A:1986 ISO 1854:1999 IDF 5:2004 | ISO 1735:2004

Whey cheeses including Whey cheeses Total fat by concentration Whey cheeses by coagulation Total fat

Calculation from fat content and dry I matter content Gravimetry (Rse Gottlieb) Gravimetry, drying at 88 C Gravimetry (Rse Gottlieb) I Gravimetry (Schmid-BondzynskiRatzlaff I

39

Creamed whey cheese

Fat on the dry basis

Skimmed whey cheese

Fat on the dry basis

Whey powders Whey powders Whey powders Whey Powders

Ash Copper Copper Lactose

IDF 59 A: 1986 | ISO 1854: 1999 and IDF 58:2004 | ISO 2920:2004 IDF 59 A:1986 | ISO 1854:1999 and IDF 58:2004 | ISO 2920:2004 ISO 5545|IDF 90:2008 AOAC 985.35 ISO 5738|IDF 76:2004 ISO 5765-1/2|IDF 79-1/2:2002

Calculation from fat content and dry matter content

Calculation from fat content and dry matter content

Gravimetry (ashing at 825C) Atomic absorption spectrophotometry Photometry (diethyldithiocarbamate) Enzymatic method: Part 1 - Glucose moiety or Part 2 - Galactose moiety Atomic absorption spectrophotometry Gravimetry (Rse-Gottlieb) Titrimetry (Kjeldahl) Gravimetry (drying at 88 C 2C) Titrimetry, Kjeldahl digestion Gravimetry (drying at 87 C) Colony count at 37C Test for identification Gravimetry (drying at 102C)

IV II III II

Whey powders Whey powders Whey powders Whey powders Whey powders Whey powders Yoghurt products Yoghurt products Yoghurt products
20

Lead Milkfat Milk protein (total N x 6.38) Moisture, "Free" Protein (total N x 6.38) Water20 Lactobacillus bulgaricus & Streptococcus thermophilus Lactobacillus bulgaricus & Streptococcus thermophilus Solids, Total

AOAC 972.25 (Codex general method) ISO 1736|IDF 9:2008 ISO 8968-1/2|IDF 20-1/2:2001 / AOAC 991.20 ISO 2920|IDF 58:2004 IDF 92:1979 / ISO 5549:1978 ISO 5537|IDF 26:2004 IDF 117 | ISO 7889:2003 IDF 146 | ISO 9232:2003 IDF 151 | ISO 13580:2005

II I I IV IV I

Water content excluding the crystallized water bound to lactose (generally known as moisture content)

40

Yoghurt

Yoghurt

Streptococcus thermophilus & Lactobacillus delbrueckii subsp. Bulgaricus >= 107 cfu/g Streptococcus thermophilus & Lactobacillus delbrueckii subsp. bulgaricus >= 107 cfu/g

ISO 7889/IDF 117: 2003

Colony count at 37C Test for identification: morphological , cultural and biochemical characteristics

ISO 9232/IDF 146:2003

Natural Mineral Waters Natural mineral waters Natural mineral waters Calcium Chloride ISO 7980:1986 (confirmed 1995) Examination of Water Pollution Control. WHO Pergamon Press (1982) Vol. 2, pp. 205-208 AOAC 973.51 ISO 9297:1989 (confirmed 1994) ISO 9308-1:1990 Titrimetry (Mercuric nitrate) Titrimetry Membrane filtration Atomic absorption spectrophotometry III II

Natural mineral waters Natural mineral waters Natural mineral waters

Chloride Chloride Coliform organism, thermotolerant organism and presumptive Escherichia coli Faecal Streptococci Iron, dissolved Magnesium Magnesium Phenols Potassium

III III I

Natural mineral waters Natural mineral waters Natural mineral waters Natural mineral waters Natural mineral waters Natural mineral waters

ISO 7899-2:1984 ISO 6332:1988 (confirmed 1995) ISO 6059:1984 (confirmed 1995) ISO 7980:1986 (confirmed 1995) ISO 6439:1990 (confirmed 1995) Examination of Water Pollution Control. WHO Pergamon Press (1982) Vol.2, pp. 142-145

Membrane filtration Spectrophotometry Titrimetry Atomic absorption spectrophotometry Spectrophotometry

I II II III I II

41

Natural mineral waters

Sodium

Examination of Water Pollution Control. WHO Pergamon Press (1982) Vol.2 pp. 148-151 Examination of Water Pollution Control. WHO Pergamon Press (1982) Vol.2, pp. 151-152 ISO 6461-2:1986 (confirmed 1996) ISO 9280:1990 (confirmed 1995) Handb. Spurenanal. 1974 Membrane filtration Gravimetry

II

Natural mineral waters

Sodium

III

Natural mineral waters Natural mineral waters Natural mineral waters

Spores of sulphite-reducing anaerobes (Clostridia) Sulphates Sulphide

I III IV

Criteria applicable to health-related substances in the Standard for Natural Mineral Waters

Provision Antimony Arsenic

ML (mg/L) 0.005 0.01

Min. applicable range (mg/L) 0.0028 0.0056

LOD LOQ (mg/L) (mg/L) 0.001 0.002 0.002 0.004

Precision Recover RSDR (%) y No more than (%) 44 80-110 44 90-107

Suggested methods Principle meeting the criteria ISO 17294-2:2003 ISO 15586:2003 EPA 200.8 ISO 17294-2:2003 ISO 15586:2003 ISO 11969:1996 EPA 200.8 ISO 11885:2007 ISO 17294-2:2003 EPA 200.8 ICP-MS GF-AAS ICP-MS ICP-MS GF-AAS AAS (Hydride) ICP-MS ICP-OES ICP-MS ICP-MS

Barium

0.7

0.35

0.07

0.14

34

95-105

42

Provision

ML (mg/L) 5 0.003

Min. applicable range (mg/L) 3.1 0.0017

LOD LOQ (mg/L) (mg/L) 0.5 1

Precision Recover RSDR (%) y No more than (%) 25 44 97-103 80-110

Suggested methods Principle meeting the criteria ISO 9390:1990 ISO 11885:2007 ISO 17294-2:2003 ISO 11885:2007 ISO 17294-2:2003 ISO 15586:2003 ISO 5961:1994 (Section 3) EPA 200.8 ISO 11885:2007 ISO 17294-2:2003 ISO 15586:2003 ISO 18412:2005 (Cr VI) ISO 23913:2006 (Cr VI) ISO 9174:1998 (Section 4) EPA 200.8 ISO 11885:2007 ISO 17294-2:2003 ISO 15586:2003 ISO 8288:1986 EPA 200.8 ISO 14403:2002 ISO 6703-1:1998 ISO 10304-1:2007 ISO 10359-1:1992 (dissolved fluoride) ISO 10359-2:1994 (inorganic bound) ISO 17294-2:2003 Spectrophotometry ICP-OES21 ICP-MS21 ICP-OES ICP-MS GF-AAS AAS ICP-MS ICP-OES ICP-MS GF-AAS Photometric CIA, spectrophotometry AAS ICP-MS ICP-OES ICP-MS GF-AAS Flame-AAS ICP-MS CFA Photometric, trimetric HPLC Electrochemical probe Digestion, distillation ICP-MS

Borate Cadmium

0.0006 0.0012

Chromium

0.05

0.028

0.01

0.02

44

90-107

Copper

0.52

0.1

0.2

32

97-103

Cyanide Fluoride

0.07 1.0

0.039 0.52

0.014 0.1

0.028 0.2

44 32

90-107 97-103

Lead
21

0.01

0.0056

0.002

0.004

44

90-107

Total Boron is determined

43

Provision

ML (mg/L)

Min. applicable range (mg/L) 0.18

LOD LOQ (mg/L) (mg/L)

Precision Recover RSDR (%) y No more than (%) 37 95-105

Suggested methods Principle meeting the criteria ISO 15586:2003 EPA 200.8 ISO 11885:2007 ISO 17294-2:2003 ISO 15586:2003 EPA 200.8 EN 1483:2007 ISO 17852:2006 ISO 5666:1999 ISO 16590:2000 EPA 200.8 ISO 17294-2:2003 ISO 15586:2003 EPA 200.8 ISO 10304-1:2007 ISO 13395:1996 ISO 7890-3:1988 ISO 10304-1:2007 ISO 13395:1996 ISO 6777:1984 ISO 17294-2:2003 ISO 15586:2003 ISO 9965:1993 EPA 200.8 GF-AAS ICP-MS ICP-OES ICP-MS GF-AAS ICP-MS AAS Enrichment by amalgamation (III) AFS AAS after tin(II) chloride reduction Enrichment by amalgamation (III) ICP-MS ICP-MS GF-AAS ICP-MS LC of ions UV CFA, FIA, Spectrophotometry Spectrophotometry LC of ions UV CFA, FIA, Spectrophotometry Spectrophotometry ICP-MS GF-AAS AAS (Hydride) ICP-MS

Manganese

0.4

0.04

0.08

Mercury

0.001

0.00056

0.0002 0.0004

44

80-110

Nickel Nitrate Nitrite

0.02 50 0.1

0.011 37 0.03

0.004 5 0.01

0.008 10 0.02

44 18 44

90-107 98-102 95-105

Selenium

0.01

0.0056

0.002

0.004

44

90-107

44

Performance characteristics of suggested methods Provision Surface active agents Mineral oil (hydrocarbon index) PCB Pesticide (organochlorine) PAH ML Applicable range- from: 0.05 5.0 mg/L >0.1 mg/L >15 ng/L > 15 ng/ L 0.005 g/L 0.04 g/L 0.005 g/L LOD 0.05 mg/l RSDR (%) < 44 < 41 <20 <20 <10 <18 <19 Recovery (%) 70-100 71-102 70-130 70-130 80-110 80-110 80-100 Suggested methods ISO 16265:2009 ISO 9377-2:2000 AOAC 990.06 AOAC 990.06 ISO 17993:2004 ISO 7981-1:2005 ISO 7981-2:2005 Principle CFA GC GC ECD GC ECD HPLC FD TLC HPLC

Processed Fruits and Vegetables Commodity Standard Processed fruits and vegetables Processed fruits and vegetables Processed fruits and vegetables Processed fruits and vegetables Processed fruits and vegetables Provision Benzoic acid Benzoic acid Calcium Drained Weight Fill of containers Method NMKL 124 (1997) NMKL 103 (1984); or AOAC 983.16 AOAC 968.31 AOAC 968.30 (Codex General Method) CAC/RM 46-1972 (reference to metal containers deleted and refer to ISO 90.1:1999 for determination of water capacity in metal containers) AOAC 972.25 (Codex general method) AOAC 932.12 ISO 2173:1978 Principle Liquid Chromatography Gas Chromatography Complexometry/ Titrimetry Sieving Gravimetry Weighing Type II III II I I

Processed fruits and vegetables Processed fruits and vegetables

Lead Packing medium Canned berry fruits (raspberry, strawberry)

AAS (Flame absorption) Refractometry

III I

45

Processed fruits and Vegetables (except canned bamboo shoots, pH determined by AOAC 981.12) Processed fruits and vegetables Processed fruits and vegetables Processed fruits and vegetables Processed fruits and vegetables Processed fruits and vegetables Processed fruits and vegetables Processed fruits and vegetables Aqueous Coconut Products Aqueous Coconut Products Aqueous Coconut Products

pH

ISO 1842:1991

Potentiometry

IV

pH pH Soluble solids Sorbates Sorbates Tin Total solids Total Fats Total solids

AOAC 981.12 NMKL 179:2005 ISO 2173:2003 AOAC 932.12 NMKL 103 (1984) / AOAC 983.16 NMKL 124 (1997) AOAC 980.19 (Codex general method) AOAC 920.151 ISO 1211:1999 IDF 1D: 1996 ISO 6731:1989 IDF 21B: 1987 ISO 1211:1999 IDF 1D: 1996 and ISO 6731:1989 IDF 21B: 1987 ISO 6731:1989 IDF 21B: 1987 CAC/RM 39-1970 CAC/RM 45-1972 CAC/RM 48-1972

Potentiometry Potentiometry Refractometry Gas Chromatography Liquid Chromatography AAS Gravimetry Gravimetry (Rse-Gottlieb) Gravimetry

III II I III II II I I I I

Non-fat solids

Calculation: Gravimetry (Rse-Gottlieb) Gravimetry I I I I

Aqueous Coconut Products Canned green beans and wax beans Canned green peas Canned green peas

Moisture Tough strings Proper fill (in lieu of drained weight) Types of peas, distinguishing

Calculation: Gravimetry Stretching Pouring and measuring Visual inspection

46

Canned mangoes Canned mushrooms Canned palmito Canned Stone Fruits Canned Stone Fruits Canned strawberries Canned strawberries Certain canned citrus fruits Certain canned citrus fruits Certain Canned Vegetables (palmito) Citrus marmalade Dates Dates Desiccated coconut Desiccated coconut Desiccated coconut Desiccated coconut Desiccated coconut Dried apricots Dried apricots Dried apricots

Syrup Washed drained weight Mineral impurities Drained weight Soluble solids Calcium Mineral impurities Calcium Calcium Mineral impurities (sand) Calcium Identification of defects Moisture Total acidity of the extracted oil Ash Extraneous vegetable matter Moisture Oil content Identification of defects Moisture Sulphur dioxide

AOAC 932.14C CAC/RM 44-1972 ISO 762:1982 (confirmed 1992) AOAC 968.30 ISO:2173:1978 AOAC 932.14C AOAC 968.31 AOAC 971.33 NMKL 153:1996 AOAC 968.31 AOAC 971.33 ISO 762:2003 AOAC 968.31 Described in the Standard AOAC 934.06 ISO 660:2009 or AOCS Cd 3d-63 (09) AOAC 950.49 Described in the Standard AOAC 925.40 AOAC 948.22 Described in the Standard AOAC 934.06 AOAC 963.20

Brix spindle method Sieving Gravimetry Gravimetry Refractometry Complexometric titrimetry Gravimetry Atomic Absorption Spectrophotometry Complexometry Titrimetry Gravimetry Complexometric titrimetry Visual inspection Gravimetry (vacuum oven) Titrimetry Gravimetry Counting extraneous material with the naked eye Gravimery (loss on drying) Gravimetry Visual inspection (weighing) Gravimetry (vacuum oven) Colorimetry

I I I I I II I II III I II I I I I IV I I I I II

47

Jams (fruit preserves) and jellies Jams (fruit preserves) and jellies Mango chutney Pickled cucumbers Pickled cucumbers Pickled cucumbers Pickled cucumbers Pickled cucumbers Preserved tomatoes Preserved tomatoes Preserved tomatoes Preserved tomatoes Processed tomato concentrates Processed tomato concentrates Processed tomato concentrates Processed tomato concentrates Processed tomato concentrates Processed tomato concentrates Raisins Raisins Raisins

Fill of Containers Soluble solids Ash insoluble in HCl Acidity, total Drained weight Mineral impurities Salt in brine Volume fill by displacement Calcium Calcium Minimum Drained Weight

CAC/RM 46-1972 ISO 2173:2003 AOAC 932.12 ISO 763:1982 AOAC 942.15 AOAC 968.30 AOAC 971.33 AOAC 971.27 (Codex general method) Described in the Standard AOAC 968.31 NMKL 153:1996 AOAC 968.30

Weighing Refractometry Gravimetry Titrimetry Gravimetry Gravimetry Potentiometry Displacement Complexometric titrimetry Atomic Absorption Spectrophotometry Gravimetry (sieving) note: Use a No. 14 screen instead of 7/16 or No. 8 Howard mould count Enzymatic determination Gravimetry Howard mould count Refractometry Potentiometry Refractometry Ashing Extraction and separation on alumina Electrical conductance

I I I I I I II I III II I

Mould count Lactic acid Mineral impurities (sand) Mould count Natural tomato soluble solids Sodium chloride Tomato soluble solids Mineral impurities Mineral oil Moisture

AOAC 965.41 EN 2631:1999 AOAC 971.33 AOAC 965.41 AOAC 970.59 AOAC 971.27 (Codex general method) AOAC 970.59 CAC/RM 51-1974 CAC/RM 52-1974 AOAC 972.20

I II IV I I II I I II I

48

Raisins Raisins Table olives Table olives Table olives Unshelled pistachio nuts Unshelled pistachio nuts Unshelled pistachio nuts Quick Frozen Fruits and Vegetables Quick frozen fruits and vegetables Quick frozen fruits and vegetables Quick frozen fruits and vegetables: Berries, leek and carrot Quick frozen fruits and vegetables: Berries, Whole kernel corn and Cornon-the-cob Quick frozen fruits and vegetables: Peaches and berries Quick frozen fruits and vegetables: Vegetables Quick frozen French fried potatoes Quick frozen green and wax beans Quick frozen peas Quick frozen spinach

Sorbitol Sulphur dioxide Acidity of brine pH of brine Salt in brine Identification of defects Moisture Size classification

AOAC 973.28 AOAC 963.20 Described in the Standard Described in the Standard AOAC 971.27 (Codex general method) Described in the Standard AOAC 925.40 Described in the Standard

Gas chromatography Colorimetry Titrimetry Potentiometry Potentiometry Visual inspection Gravimetry (loss on drying) Number per 500 g

II II IV IV II I I I

Net weight Thawing procedure Mineral impurities Soluble solids, total

CAC/RM 34-1970 CAC/RM 32-1970 CAC/RM 54-1974 CAC/RM 43-1971

Weighing Thawing Flotation and sedimentation Refractometry

I I I I

Drained fruit/drained berries Cooking procedure Moisture Tough strings Solids, alcohol insoluble Dry matter, Salt-free

Described in the Standards CAC/RM 33-1970 AOAC 984.25 CAC/RM 39-1970 CAC/RM 35-1970 Described in the Standard

Draining Cooking Gravimetry (convection oven) Stretching Gravimetry Weighing

I I I I I I

49

Processed Meat and Poultry Products and Soups and Broths Meat Products Meat Products Processed meat and poultry products Processed meat and poultry products Processed meat and poultry products Processed meat and poultry products Processed meat and poultry products Processed meat and poultry products Bouillons and Consomms (soups and broths) Bouillons and Consomms (soups and broths Bouillons and Consomms (soups and broths Nitrates and/or Nitrites Nitrates and/or Nitrites Fat Lead Nitrates Nitrites Tin Nitrogen/protein Amino nitrogen Creatinine Nitrogen, total ENV 12014-3:1998-06 - Part 3 ENV 12014-4:1998-06 - Part 4 NMKL 165 (2000) ISO 1443-1973 AOAC 934.07 ISO 3091:1975 (confirmed 1996) ISO 2918:1975 (confirmed 1996) AOAC 985.16 (Codex general method) ISO 937:1978 (confirmed 1995) AIIBP Method No 2/7 AIIBP Method No 2/5 AOAC 928.08 AIBP Method No 2/4 AOAC 972.25 (Codex general method) AOAC 973.31 (Codex general method) ISO 2918:1975 (confirmed 1996) AOAC 985.16 (Codex general method) ISO 1443-1973 Spectrometric determination of nitrate and nitrite content of meat products after enzymatic reduction of nitrate to nitrite Ion-exchange chromatographic method Gravimetry Colorimetry (dithizone) Colorimetry (cadmium reduction) Colorimetry Atomic absorption spectrophotometry Titrimetry Volumetry (modified Van Slyke) HPLC Kjeldahl Potentiometric titration (chloride expressed as sodium chloride) Atomic absorption spectrophotometry Colorimetry Colorimetry Atomic absorption spectrophotometry Gravimetry (extraction) III

III I II II IV II II II II II II II II IV II I

Bouillons and Consomms (soups and Sodium chloride broths) Canned corned beef Canned corned beef Canned corned beef Canned corned beef Cooked cured chopped meat Lead Nitrites, potassium and/or sodium salt Nitrites, potassium and/or sodium salt Tin (Products in tinplate and other containers) Fat

50

Cooked cured chopped meat Cooked cured chopped meat Cooked cured chopped meat Cooked cured chopped meat Cooked cured ham Cooked cured ham Cooked cured ham Cooked cured ham Cooked cured ham Cooked cured ham Cooked cured ham Cooked cured pork shoulder Cooked cured pork shoulder Cooked cured pork shoulder Cooked cured pork shoulder Cooked cured pork shoulder Cooked cured pork shoulder Cooked cured pork shoulder Luncheon meat Luncheon meat Luncheon meat Luncheon meat Luncheon meat

Lead Nitrites Nitrites Tin Fat Gelatin, added Lead Nitrites Nitrites Protein (conversion factor 6.25) Tin Fat Gelatin, added Lead Nitrites Nitrites Protein Tin Fat Lead Nitrites, potassium and/or sodium salt Nitrites, potassium and/or sodium salt Tin

AOAC 972.25 (Codex general method) AOAC 973.31 (Codex general method) ISO 2918:1975 (confirmed 1996) AOAC 985.16 (Codex general method) ISO 1443:1973 Described in the Standard AOAC 972.25 (Codex general method) AOAC 973.31 (Codex general method) ISO 2918:1975 (confirmed 1996) ISO 937:1978 (confirmed 1995) AOAC 985.16 (Codex general method) ISO 1443:1973 Described in the Standard AOAC 972.25 (Codex general method) AOAC 973.31 (Codex general method) ISO 2918:1975 (confirmed 1996) ISO 937:1978 (confirmed 1995) AOAC 985.16 (Codex general method) ISO 1443:1973 AOAC 972.25 (Codex general method) AOAC 973.31 (Codex general method) ISO 2918:1975 (confirmed 1996) AOAC 985.16 (Codex general method)

Atomic absorption spectrophotometry Colorimetry Colorimetry Atomic absorption spectrophotometry Gravimetry (extraction) Calculation Atomic absorption spectrophotometry Colorimetry Colorimetry Titrimetry, Kjeldahl digestion Atomic absorption spectrophotometry Gravimetry (extraction) Calculation Atomic absorption spectrophotometry Colorimetry Colorimetry Titrimetry, Kjeldahl digestion Atomic absorption spectrophotometry Gravimetry (extraction) Atomic absorption spectrophotometry Colorimetry Colorimetry Atomic absorption spectrophotometry

II II IV II I I II II IV II II I I II II IV II II I II II IV II

51

Sugars and Honey Honey Acidity MAFF Validated Method V19 J. Assoc. Public Analysts (1992) 28 (4) 171175 AOAC 969.38B or MAFF Validated Method V21 AOAC 920.180 Titrimetry I

Honey

Moisture

Refractometry

Honey Honey

Sample preparation Solids, water-insoluble

MAFF Validated Method V22 Gravimetry J. Assoc. Public Analysts (1992) 28(4) 189193 AOAC 998.18 AOAC 978.17 ISO 5377:1981 ISO 1741:1980 ISO 5809:1982 Carbon isotope ratio mass spectrometry Carbon isotope ratio mass spectrometry Titrimetry Gravimetry (vacuum oven) Single sulphonation

Honey Honey Sugars (dextrose anhydrous and dextrose monohydrate) Sugars (dextrose anhydrous and dextrose monohydrate) Sugars (dextrose anhydrous and dextrose monohydrate, dried glucose syrup, glucose syrup, powdered dextrose, lactose) Sugars (dextrose anhydrous and dextrose monohydrate) Sugars (fructose) Sugars (fructose) Sugars (fructose)

Sugars added (for sugar profile) Sugars added: detection of corn and cane sugar products D-Glucose Solids, total Sulphated ash

I I I I I

Sulphur dioxide pH Conductivity ash D-Fructose

ISO 5379:1983 ICUMSA GS 1/2/3/4/7/8-23 (1994) ICUMSA GS 2/3-17 (1994) ISO 10504:1988

Acidimetry and nephelometry Potentiometry Conductimetry Liquid chromatography (refractive index detection)

IV I I II

52

Sugars (fructose) Sugars (fructose) Sugars (fructose) Sugars (glucose syrup and dried glucose syrup) Sugars (glucose syrup and dried glucose syrup) Sugars (glucose syrup and dried glucose syrup) Sugars (lactose) Sugars (lactose) Sugars (lactose) Sugars (plantation and mill white sugar)

D-Glucose Loss on drying Sulphur dioxide Reducing sugar Solids, total Sulphur dioxide Lactose, anhydrous Loss on drying pH Colour

ISO 10504:1988 ISO 1742:1980 ISO 5379:1983 ISO 5377:1981 ISO 1742:1980 ISO 5379:1983 ICUMSA GS 4/3-3 (1994) USP General Chapter 731 ICUMSA GS 1/2/3/4/7/8-23 (1994) ICUMSA GS9/1/2/3-8 ICUMSA GS 1/3/4/7/8-13 (1994) ICUMSA GS 1/3/7-3 (1994) ICUMSA GS 2/1/3-15 (1994) ICUMSA GS 1/2/3-1 (1994) ICUMSA GS 2/3-35 (1998) NMKL 135 (1990) EN 1988-2 (1998) ICUMSA GS 2/3-35 (1998) NMKL 135 (1990) EN 1988-2 (1998) ICUMSA GS 2/3-9 (1994) ICUMSA GS 2/3-17 (1994)

Liquid chromatography (refractive index detection) Gravimetry Acidimetry and nephelometry Titrimetry Gravimetry (vacuum oven) Acidimetry and nephelometry Titrimetry Gravimetry (Drying at 120C for 16 h) Potentiometry Photometry Conductimetry Titrimetry (Lane & Eynon) Gravimetry Polarimetry Enzymatic method

II I IV I I IV II I I I I I I II II

Sugars (plantation or mill white sugar) Conductivity ash Sugars (plantation or mill white sugar) Invert sugar Sugars (plantation or mill white sugar) Loss on drying Sugars (plantation or mill white sugar) Polarization Sugars (plantation or mill white sugar) Sulphur dioxide

Sugars (powdered sugar and powdered Sulphur dioxide dextrose) Sugars (powdered sugar) Sugars (powdered sugar) Colour Conductivity ash

Enzymatic method

II

Photometry Conductimetry

I I

53

Sugars (powdered sugar) Sugars (powdered sugar) Sugars (powdered sugar) Sugars (raw cane sugar)

Invert sugar Loss on drying Polarization Sulphur dioxide

ICUMSA GS 2/3-5 (1997) after filtration if necessary to remove any anticaking agents ICUMSA GS 2/1/3-15 (1994) ICUMSA GS 2/3-1 after filtration if necessary to remove any anticaking agents ICUMSA GS 2/3-35 (1998) NMKL 135 (1990) EN 1988-2 (1998) ICUMSA GS 1/3/4/7/8-13 (1994) ICUMSA GS 4/3-3 (1994) (applicable at levels >10% m/m) ICUMSA GS 1/3/7-3 (1994) (applicable at levels <10% m/m) ICUMSA GS 2/1/3-15 (1994) ICUMSA GS 4/3-7 (1994) ICUMSA GS 1/3/4/7/8-11 (1994) ICUMSA GS 2/3-35 (1998) NMKL 135 (1990) EN 1988-2 (1998) ICUMSA GS 2/3-9 (1994) ICUMSA GS 2/3-17 (1994) ICUMSA GS 2/3-5 (1997) ICUMSA GS 2/1/3-15 (1994) ICUMSA GS 2/3-1 (1994)

Titrimetry Gravimetry Polarimetry Enzymatic method

I I II II

Sugars (soft white sugar and soft brown sugar) Sugars (soft white sugar and soft brown sugar) Sugars (soft white sugar and soft brown sugar) Sugars (soft white sugar and soft brown sugar) Sugars (soft white sugar and soft brown sugar) Sugars (soft brown sugar) Sugars (soft white sugar and soft brown sugar) Sugars (soft white sugar) Sugars (white sugar) Sugars (white sugar) Sugars (white sugar) Sugars (white sugar)

Conductivity ash Invert sugar Invert sugar Loss on drying Sucrose plus invert sugar Sulphated ash Sulphur dioxide

Conductimetry Titrimetry (Lane & Eynon) Titrimetry (Lane & Eynon) Gravimetry Titrimetry Gravimetry Enzymatic method

I I I I I I II

Colour Conductivity ash Invert sugar Loss on drying Polarization

Photometry Conductimetry Titrimetry Gravimetry Polarimetry

I I I I II

54

Sugars (white sugar)

Sulphur dioxide

ICUMSA GS 2/3-35 (1998) NMKL 135 (1990) EN 1988-2 (1998)

Enzymatic method

II

Miscellaneous Products Chili sauce Chili sauce Chili sauce Edible cassava flour Edible cassava flour Edible cassava flour Fermented Soybean Paste Fermented Soybean Paste Fermented Soybean Paste Food grade salt Food grade salt Food grade salt Food grade salt Food grade salt
22

pH pH Fill of containers Fibre, crude Granularity Moisture Total Nitrogen Amino Nitrogen Moisture Arsenic Cadmium Calcium and magnesium Copper Halogens

NMKL 179:2005 (Codex general method) AOAC 981.12 (Codex general method) CAC/RM 46-1972 (Codex general method) ISO 5498:1981 (B.5 separation) ISO 2591-1:1988 ISO 712:1998 AOAC 984.13 AOAC 920.154 on the conditions specified in the standard 22 AOAC 934.01 (70C, 50 mm Hg) ESPA/CN-E/105-1996 ESPA/CN-E/107-1997 ISO 2482:1973 ESPA/CN-E/101-1994 ISO 2481:1973

Potentiometry Potentiometry Weighing Gravimetry Sieving Gravimetry Kjeldahl Volumetry Gravimetry Photometry Atomic absorption spectrophotometry Complexometric titrimetry Photometry Mercurimetry

II III I I I I I I I IV IV II II II

Section 9.2 Determination of Amino Nitrogen

Preparation of test samples: Weigh 2 g of sample into a 250 ml beaker and mix the sample with 100 ml of cold (15C) NH3-free H2O and then stir the mixture for 60 min. Next, decant the mixture through a quantitative filter and collect the filtrate in a 100 ml volumetric flask. Endpoint - A pH meter shall be used to determine the endpoint instead of optical verification of colours

55

Food grade salt Food grade salt Food grade salt Food grade salt Food grade salt Food grade salt Food grade salt

Insoluble matter Iodine Iodine Lead Loss on drying Mercury Potassium

ISO 2479:1972 ESPA/CN-E/109-1994 AOAC 925.56 ESPA/CN-E/108-1994 ISO 2483:1973 ESPA/CN-E/106-1994 ESPA/CN-E/104-1994 (applicable to products containing 2 mgK/kg)

Gravimetry Titrimetry using sodium thiosulphate Titrimetry using sodium thiosulphate Atomic absorption spectrophotometry Gravimetry (drying at 110C) Cold vapour atomic absorption spectrophotometry Flame atomic absorption spectrophotometry

II II III IV I IV II

Food grade salt

Potassium

ESPA/CN-E/103-1994 Titrimetry (applicable to products containing 100 mgK/kg) Described in the Standard ISO 2480:1972 AOAC 945.68 AOAC 971,27 NMKL 178:2004 AOAC 968.30 ISO 2171:1993 ISO 5498:1981 (B.5 separation) ISO 2591-1:1988 ICC Method No 109/1 (1986) ISO 712:1998 AOAC 925.45 Potentiometry Sieving Gravimetry Gravimetry Sieving Gravimetry Gravimetry, drying at atmospheric pressure Calculation Gravimetry

III

Food grade salt Food grade salt Foul medames Foul medames Foul medames Gari Gari Gari Gari Ginseng Products

Sodium chloride Sulphate Sample Preparation Salt content Drained weight Ash Fibre, crude Granularity Moisture Moisture

I II _ II I I I I I IV

56

Ginseng Products Ginseng Products Ginseng Products Ginseng Products Ginseng Products Gochujang Gochujang Gochujang Gochujang Guideline level for acrylonitrile Guideline levels for mercury in fish Guideline levels for vinyl chloride monomer Guideline levels for vinyl chloride monomer Guidelines for nutrition labelling Guidelines for nutrition labelling Guidelines for nutrition labelling Humus with tehena Humus with tehena

Solids Ash Water-insoluble Solids Water-saturated 1-butanol extracts Identification of ginsenosides Rb1 and Rf Capsaicin Crude protein Moisture Acrylonitrile Methyl mercury Vinyl chloride monomer Vinyl chloride monomer Polyunsaturated fatty acids Saturated fat Saturated fatty acids Sample Preparation Salt content

AOAC 925.45 and calculated by subtracting calculation the content of water from 100%. AOAC 923.03 described in the Standard (Annex A) described in the Standard (Annex B) described in the Standard (Annex C) AOAC 995.03 described in the Standard (Annex D) AOAC 984.13 (Nitrogen conversion factor: 6.25) AOAC 934.01 ( 70C, 50 mm Hg) AOAC 985.13 AOAC 988.11 ISO 6401:1985 Gravimetry, after ashing at 550C Gravimetry Gravimetry TLC or HPLC HPLC Gas chromatography Kjeldahl Gravimetry Gas chromatography Atomic absorption spectrophotometry Gas chromatography

IV IV IV IV IV II IV I I II II II III II II II _

Commission Directive 81/432/EEC O.J. No. Gas chromatography ("head-space") L.167, p. 6, 24.6.81 AOCS Ce 1h-0523 Gas liquid chromatography AOAC 996.06; or AOCS Ce 1h-05 Gas liquid chromatography AOCS Ce 1h-05 AOAC 945.68 AOAC 971.27 NMKL 178:2004 Potentiometry Gas liquid chromatography

II

23

Can also be used to measure trans unsaturated fatty acids

57

Humus with tehena Sago Flour Sago Flour Sago Flour Sago Flour Sago Flour Tehena Tehena Tehena Tehena Tehena Tehena

Total acidity Moisture Content Ash (inorganic extraneous matter) Acidity Crude Fibre Starch Moisture Content Protein content Total Ash Acid Insoluble Ash Total Acidity Sesame oil

AOAC 925.53 ISO 712:1998 ISO 2171: 2007 AOAC 939.05 ISO 6541:1981 AOAC 920.44. ISO 934:1980 ISO 1871:1975 ISO 6884:1980 ISO 735:1977 ISO 729:1988 AOCS Cb 2-40 (97) (Baudouin Test)

Titrimetry Gravimetry Gravimetry Titrimetry Gravimetry Gravimetry Gravimetry Titrimetry, Kjeldahl Gravimetry Gravimetry Titrimetry Colour reaction

I I I I I I I I I I I I

58

PART B METHODS OF SAMPLING BY COMMODITY CATEGORIES AND NAMES

Commodity Categories Cereals, Pulses and Legumes and Derived Products Wheat protein products including wheat gluten Fats and Oils Olive Oils and Olive-Pomace Oils Milk and Milk Products Milk products Milk products Milk products Processed Fruits and Vegetables Desiccated coconut Certain canned vegetables, jams and jellies Chili sauce

Method of Sampling

Notes

ISO 13690:1999 ISO 661:1989 and ISO 5555:2001. ISO 707|IDF 50:2008 ISO 5538|IDF 113:2004 IDF 136A:1992 ISO 8197:1988 Described in the Standard Described in the Standards Described in the Standard General instructions for obtaining a sample from a bulk Inspection by attributes Inspection by variables

59