Thin Layer Chromatography September 16, 2009 Purpose: - To separate and characterize the polarity of a mixture of benzene derivatives

and
analgesics.

Procedure: - The handout procedure1 was followed and used .3% of acetic acid. Material: UV light, 9.95 ml Ethyl acetate, micro capillary, 10 ml Hexane, 250ml Beaker, foilpaper, filter paper. DATA Table II % of Acetic Acid .3 .4 .5 .6 .7 Table III % of Acetic Acid .4 .5 .6 .7 RF Value Acetaminophen .210 .083 .159 .276 .169

RF Value Caffeine .0998 .542 .062 .069 .062

0.6 0.4 0.2 0 0 0.2 0.4 RF Values 0.6 0.8

Rf Value
0.4 0.2 0 0 0.2 0.4 0.6 0.8 Rf Value

Table IV % of Acetic Acid .3 .4 .5 .6 .7 RF Value aspirin .663 (.140) .583 .513 .558 .662

Rf Value
1 0 0 0.2 0.4 0.6 0.8 Rf Value

Percent Recovery Caffeine Percent Recovery =(.067/.167)*100 =40.2% Acetaminophen Percent recovery = (.0306/.141)*100 = 21.7 Aspirin Percent Recovery: -(.0672/.66)*100 =10.3% Discussion: the purpose was to use thin layer chromatography to separate and characterize the polarity of a mixture of benzene derivatives and analgesics. Result shows that Caffeine is most polar >Aspirin >Acetaminophen. The results are little different the groups hypothesis. Group hypothesis was Caffeine >Aspirin >Acetaminophen; the only experiment disagrees on is Acetaminophen, which could be an error in experiment. There was no unknown caffeine collected, so there was no error calculated between the pure caffeine and unknown caffeine. According to the substituent functional group analysis, acetaminophen has more functional group, thus it should have been more polar and the Rf value for Acetaminophen should be less. Conclusion: Group determined that the chemicals Caffeine is most polar based on Rf value collected, Substituents analysis, and solubility Spotting. Yet the overall experiment proved 75% correct and the hypothesis was almost correct. Using TLC Spotting technique the group learned to separate out the chemical via their polarity and solubility. The experiment was quite successful.