Final&Study&Guide&&

Microbiology&

Ascaris lumbricoides (eggs)- NematodeHELMINTHES

Ascaris lumbricoides - Nematode- Helminthes

Aspergillus Condia- FUNGI

Bacillus

Balantidium coli- Phylum Cilliophora- Protozoa

Candida albicans (budding Yeast) FUNGI

Capsule stain

Clostridium

Endospore

ENTERIC

Enterobius vermicularis - NematodeHELMINTHES

Entamoeba histolytica - Subphylum sarcodinaProtozoa

Fasciola hepatica- Trematodes- HELMINTHES

Flagella

Giardia lamblia- Sarcomastigophora:Subphylum Mastigophora - PROTOZOA

Nisseria

Plasmodium vivax - ApicomplexansPROTOZOA

Penicillium notatum- FUNGI

Schistosoma japonicum -TrematodesHELMINTHES

Schistosoma mansoni (eggs) - TrematodesHELMINTHES

Staph

Strept

Taenia solium- Cestodes- HELMINTHES

TRICHOMONAS vaginalis -Sarcomastigophora PROTOZOA-

Beta-hemolysin breaks down the red blood cells and hemoglobin completely. This leaves a clear zone around the bacterial growth.

Alpha-hemolysin partially breaks down the red blood cells and leaves a greenish color behind

If the organism does not produce hemolysins and does not break down the blood cells, no clearing will occur. This is called -hemolysis (gamma hemolysis).

POSITIVE

NEG

Bacterial Susceptibility test on Blood Agar

Bile esculin agar contains oxgall (bile salts) to inhibit the growth of gram positive organisms other than enterococci and group D streptococci.

(MSA) is a selective and differential medium. The high concentration of salt (7.5%) selects for members of the genus Staphylococcus, since they can tolerate high saline levels. If an organism can ferment mannitol, an acidic byproduct is formed that will cause the phenol red in the agar to turn yellow.

Coagulase&Test&

Urea&Test&

+&
The coagulase test identies whether an organism produces the exoenzyme coagulase, which causes the brin of blood plasma to clot.

=&

9&

differential medium that tests the ability of an organism to produce an exoenzyme, called urease, that hydrolyzes urea to ammonia and carbon dioxide.

selective and differential medium designed to isolate and differentiate selective and differential medium used to isolate fecal coliforms enterics based on their ability to ferment lactose. Bile salts and crystal Green=fermentation+acid Pink= Fermentation little acid violet inhibit the growth of Gram positive organisms

Indole&test&

Glucose&Fermenta@on&

N/R&

Sim Medium used to detect enterics

Methyl&Red&Test&
The Methyl Red test involves adding the pH indicator methyl red to an inoculated tube of MR-VP broth. If the organism uses the mixed acid fermentation pathway and produces stable acidic end-products, the acids will overcome the buffers in the medium and produce an acidic environment in the medium. When methyl red is added, if acidic end products are present, the methyl red will stay red.

SIM&Tubes&

SIM medium is a combination differential medium that tests three different parameters, which are represented by the three letters in the name: Sulfur Reduction Indole Production Motility

used to detect acetoin in a bacterial broth culture. The test is performed by adding alphanaphthol and potassium hydroxide to the Voges-Proskauer broth which has been inoculated with bacteria. A cherry red color indicates a positive result, while a yellow-brown color indicates a negative result.

NO REACTION
POSSITIVE

+ IODINE

Starch agar is a differential medium that tests the ability of an organism to produce certain exoenzymes, A clearing around the bacterial growth indicates that the organism has hydrolyzed starch.

The oxidase test identies organisms that produce the enzyme cytochrome oxidase. If the test organism produces cytochrome oxidase, the oxidase reagent will turn blue or purple within 15 seconds.

Lactose&Fermenta@on&

+&Gas&&&&&&&&&+&&&&&&&&&&&&&&&=&