Name: OBFENDA, Nicolo Dominique R.

Course and Section: BIO20 1 / A3 Program & Year: ECE-2

Date Performed: December 12, 2012 Date Submitted: December 13, 2012 Professor: Suico

A. FASTA SEARCH

STKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQSGVGAL FNGINVLQAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSE YEYPVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSM TAPTGSKPSFPDGMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQ VFLQPLNPQYPMIPCNESCSVVGKVIASQWPEETFG 1. Which protein can be assigned to this sequence? Give the top 3 proteins that are the most similar to the sequence.
Entry
H7C6D3

Entry name
H7C6D3_CORGL

Status
unreviewed

Protein names
Cystathionine beta-Lyase (EC 4.4.1.-) (EC 4.4.1.8) Cystathionine beta-lyase PLP-dependent aminotransferase (EC 2.6.1.-)

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025) Corynebacterium glutamicum (Brevibacterium saccharolyticum) Corynebacterium glutamicum K051

Length
368

Identity
100.00%

Score
1916

Evalue
0

Gene names
aecD cg2536 metC AecD

Q93QC6 I0LLS0

Q93QC6_CORGT I0LLS0_CORGL

unreviewed unreviewed

368 368

100.00% 100.00%

1916 1916

0 0

3 Proteins that are most similar: 1. Repressor OS=Escherichia coli J53 PE=4 SV=1 2. Phage lambda repressor protein CI OS=Escherichia coli GN=cI PE=1 SV=1 3. Repressor protein CI OS=Enterobacteria phage lambda GN=CI PE=1 SV=2 2. Open the DB:ID link of each protein (on the second column from the left), and based on the opened link, provide the following information: a. Description of Protein DB:ID : TR:Q7B004_ECOLX Protein Name: Phage lambda repressor protein CI Gene Name: cI Sequence Length : 237 AA Taxonomic identifier: 562 [NCBI] Sequence length : 237 AA b. Organism source of the protein : Escherichia coli

B. FASTA SEARCH

MRFPELEELKNRRTLKWTRFPEDVLPLWVAESDFGTCPQLKEAMADAVER EVFGYPPDATGLNDALTGFYERRYGFGPNPESVFAIPDVVRGLKLAIEHF TKPGSAIIVPLPAYPPFIELPKVTGRQAIYIDAHEYDLKEIEKAFADGAG SLLFCNPHNPLGTVFSEEYIRELTDIAAKYDARIIVDEIHAPLVYEGTHV VAAGVSENAANTCITITATSKAWNTAGLKCAQIFFSNEADVKAWKNLSDI TRDGVSILGLIAAETVYNEGEEFLDESIQILKDNRDFAAAELEKLGVKVY APDSTYLMWLDFAGTKIEEAPSKILREEGKVMLNDGAAFGGFTTCARLNF ACSRETLEEGLRRIASVL 1. Which protein can be assigned to this sequence? Give the top 3 proteins that are the most similar to the sequence.
Entry
H7C6D3

Entry name
H7C6D3_CORGL

Status
unreviewed

Protein names
Cystathionine beta-Lyase (EC 4.4.1.-) (EC 4.4.1.8) Cystathionine beta-lyase PLP-dependent aminotransferase (EC 2.6.1.-)

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025) Corynebacterium glutamicum (Brevibacterium saccharolyticum) Corynebacterium glutamicum K051

Length
368

Identity
100.00%

Score
1916

Evalue
0

Gene names
aecD cg2536 metC AecD

Q93QC6 I0LLS0

Q93QC6_CORGT I0LLS0_CORGL

unreviewed unreviewed

368 368

100.00% 100.00%

1916 1916

0 0

Top 3 Proteins are: 1. Cystathionine beta-Lyase OS=Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG

3730 / NCIMB 10025) GN=aecD PE=4 SV=1

2 Cystathionine beta-lyase OS=Corynebacterium glutamicum GN=metC PE=4 SV=1 3. PLP-dependent aminotransferase OS=Corynebacterium glutamicum K051 GN=AecD PE=4 SV=1 2. Open the DB:ID link of each protein (on the second column from the left), and based on the opened link, provide the following information:

a. Description of Protein DB:ID : TR:Q93QC6_CORGT Protein Name: Corynebacterium glutamicum (Brevibacterium saccharolyticum) Gene Name : metC Taxonomic Identifier: 1718 [NCBI] Sequence Length : 368 AA
Sequence Status : Complete Protein Existence : Predicted

b. Organism source of the protein :Corynebacterium glutamicum (Brevibacterium saccharolyticum)

A. BLAST SEARCH

1. 3 Proteins that are most similar: 1. Repressor OS=Escherichia coli J53 PE=4 SV=1 2. Phage lambda repressor protein CI OS=Escherichia coli GN=cI PE=1 SV=1 3. Repressor protein CI OS=Enterobacteria phage lambda GN=CI PE=1 SV=2 2. a. Description of Protein DB:ID : TR:Q7B004_ECOLX Protein Name: Phage lambda repressor protein CI Gene Name: cI

Encoded on: Plasmid pGBG1

Sequence Length : 237 AA Taxonomic identifier: 562 [NCBI] Sequence length : 237 AA b. Organism source of the protein : Escherichia coli

B. BLAST SEARCH
a. Description of Protein DB:ID : TR:Q93QC6_CORGT Protein Name: Corynebacterium glutamicum (Brevibacterium saccharolyticum) Gene Name : metC Taxonomic Identifier: 1718 [NCBI] Sequence Length : 368 AA
Sequence Status : Complete Protein Existence : Predicted

b. Organism source of the protein :

Corynebacterium glutamicum (Brevibacterium saccharolyticum)

OVERVIEW