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Diabetes
Diabetesmellitus
isagroupofmetabolicdiseasescharacterizedbyhigh bloodsugar(glucose)levelsorhyperglycemia
Diabetesisclassifiedintwotypes:
Type1
calledinsulindependentdiabetesmellitus
Type2
noninsulindependentdiabetesmellitus
Diabetescomplications
Hyperglycemia:
Impairimmunesystem Patientssufferofincreasedlevelsofbacterialinfections (Stoeckle etal2008). Damagesthecardiovascularsystem diabeticsshowedincreaselevelsofhypercholesterolemia andhypertension(Khamaisi etal2003).
PreviousStudies
Gymnema Studies Gymnema sylvestre isstudiedforitsknownanti hyperglycemicproperties
Theextractincreasesthefunctionofbetacells(Persaud et al1999)
Previousstudies
Ethnobotanical surveyconductedinCayey region,literature,andbooksinPRidentified fourplantsusedinPuertoRicoasdiabetes therapyadjuvant.
TheseareTapeinochilus ananassae,Costus spp, Rhoeo spathacea andSyzygium jambos Bothmethanolic andaqueousextractswere tested Gymnema sylvestre extractwasusedaspositive control
Relevance
Thisisthefirstassessmentofthesafetyatthe cellularlevelofthesefourextracts
Weusedtwocellmodelsofbetacells(BetaTC6) andtheHepaticcellline(Hep G2)
CellModel:BetaCells
CellModel:Hep Cells
MTTAssay
Invitro assayforthemeasurementofcell proliferation Itdeterminesareductionincellviabilityaftera particulartreatment Cellsareculturedinflatbottomed,96welltissue cultureplates Thetetrazolium compoundMTT(3[4,5 dimethylthiazol2yl]2,5diphenyltetrazolium bromide)isthecompoundusedtomeasure viability
MTTAssay
MTTisreducedbythemitochondrial dehydrogenase,presentinactivecells Reductionallowstheformationofinsoluble purpleformazan dyecrystals Adetergentisaddedtothewellstosolubilize the crystals Absorbanceisreadusingaspectrophotometer RateofMTTreductionisproportionaltotherate ofcellproliferation
ResearchDesign
ResearchDesign
24h Incubation
Extracts (0.5 mg/mL)
ResearchDesign
24h
ResearchDesign
Absorbance 570 nm
Results
Results
Results
Results
Conclusions
Noneoftheextractspreparationstestedin Betacellsshoweddecreasecellviability Alloftheaqueousandmethanolic extracts testedwithHep cellsshowednodecreased cellviability. Theextractsrepresentpromisingcandidates forfurthertherapeutical analysesastheydo notinducecelldeath.
FutureExperiments
Testadditionalconcentrationsoftheextracts Examinetheeffectoflongtermexposure Futureinvivo assaywillbenecessaryto establishthetherapeutical benefitsofthese extractsinbetacellfunctionandin improvementofdiabetes
References
Persaud SJ,AlMajed H,RamanA,JonesPM.Gymnema sylvestre stimulatesinsulin releaseinvitrobyincreasedmembranepermeability.JEndocrinol.1999 Nov;163(2):20712. LiuB,AsareAnane H,AlRomaiyan A,HuangG,Amiel SA,JonesPM,Persaud SJ. Characterisation oftheinsulinotropic activityofanaqueousextractofGymnema sylvestre inmousebetacellsandhumanisletsofLangerhans.CellPhysiol Biochem.2009;23(13):12532. Stoeckle M,Kaech C,Trampuz A,Zimmerli W.Theroleofdiabetesmellitusin patientswithbloodstreaminfections.SwissMedWkly.2008Sep6;138(35 36):5129. Khamaisi M,WexlerID,Skrha J,Strojek K,Raz I,Milicevic Z.Cardiovasculardisease intype2diabetics:epidemiology,riskfactorsandtherapeuticmodalities.Isr Med AssocJ.2003Nov;5(11):8016.
Thanks
Collaborators BiologyDepartmentandtechnicians Nivea Franco AngieCastell RISEprogram Yadira Ortiz MSC BiochemistryDepartment Dr.CarmenCadilla Otherresources NIHRIMIProgram#1P2MD0011120 AMPprogram InstitutodeInvestigacionesInterdisciplinarias
Thanks
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