122.

231 Genes and Gene Expression

Answers to Numeracy Test 2009
1.

203 g/mol x

2.

250
0.5 mol
Lx
1000
L

25.38 g

Dilution factor = 20

100 mL
= 5 mL
20
5 mL of 0.5 M MgCl2 to 95 mL H2O

3.

0.5 mol/L x

b.

pH

a.

5 molL-1
= 5 x 10 -3 M
1000

b.

5 molL-1 x 1000 = 5000 M

c.

5 x 10 -3
x 58.5 = 0.293 g/L
mmolL-1

4.

0.3 x 2
= 0.15 M = 300 mM
1.0

a.

= -log [H+]
= -log 0.30
= 0.52

x 2 for 2mol H+

[H+] must be in M

0.293 x 1000 = 293 mg/L

5.

d.

293 x 10 3
= 0.293 g/L
10 6

a.

10 fold dilution 1 L of DNA solution + 9 L H2O

b.

10 fold dilution of (a.) 1 L of (a.) + 9 L H2O

c.

10 fold dilution of (b.) 1 L of (b.) + 9 L H2O

Serial dilutions are best, otherwise the volumes to be measured are too small
for accuracy.

moles H+ =

6.

250
L x 0.6 mol L1
1000

0.15 mole

300 mL

need 0.15 moles of OH-

0.15 mol
volume
OH- =
x 1000

0.5 mol L-1

7.

0.7g
x 15 x 75 mL = 7.87 g
100 mL

8.

Mwt =

100
x 204 x 2 = 54400 g/mol
0.75

9.

pH = pKa + log
5 = 4.77 + log

0.23 = log

[b]
[a]

[b]
[a ]

[b]
[a ]

[b]
[a ]

= 1.698

[base] =

1.698
x 0.2 M = 0.126 M
2.698

[acid] =

1
x 0.2 M = 0.074 M
2.698

10.

Mwt oligonucleotide = 18 x330

= 5940 g/mol
= 5940 g/mol
no. mol in 240 g
=

240
= 0.0404 mol
5940

volume at TE to make 500 M

0.0404 mol
x 10 6 = 81L
500 mol

11.

Tris

10mM x 10 500 L
x
x 121 g/mol = 6.05 g
1000
1000

EDTA

0.1 mM x 10
500 L
x
x 373 g/mol = 0.187 g
1000
1000

Titrate to pH 8.0 with HCl and make up to 500 mL

12.

500
= 100 fold dilution
5
75 mg in 5 L

75
x 100 = 1500 ng/L
5
= 1.5 g/L
= 1500 g/mL

Yield = 15 ng/L x 100 x 250 L

= 375000 ng
= 0.375 mg

13.

Mwt plasmid = 2500 x 660 = 1650000 g/mol

5 x 10 -6 g
mole in 5g plasmid =
1650000 g/mol
-12
= 3 x 10 mole
No. molecules in 3 x 10-12 mole
x 6.022 x 1023 molecules/mole
= 3 x 10-12 mole
= 1.82 x 1012 molecules

14.

Mwt = 106 g/mol

10 -6
moles in 1 g =
= 1 x 10 -12 mole
6
10
1 x 10-12 mole in 1mL TE
1 x 10-9 mole in 1 L
1 x 10-9 M

15

1 mole bases = 330 g/mol

1 mole of ss DNA = 106 g/mol
10 6
= 3030 bases
330
= 3.03 kb