PDO Case

Jecyca Thrinda 24031018004
Siti Fatimatul F 24031018022

Irji Rizqi S 240310180051
Yaritsha Nafhan T 240310180061
PDO case Q1 : Production Rates
Part I: Production Rates
For a 1,3 Propanediol (C3H8O2) – PDO – production organism (C1H1.8O0.5N0.2) a first

chemostat experiment is performed and measurements are made when the chemostat is at
steady state. PDO is produced aerobically using glucose (C6H12O6) as a substrate and NH4+ as
N-source. The chemostat feed Fin in L/h contains glucose and (NH4)2SO4 dissolved in water.
Air sparging, with rate FN,in in mol/h, is used to supply O2 to the organism and to remove
CO2. The dry sparged air contains much more CO2 than normal and its composition is 21%
O2; 0.5% CO2 and 78.5% N2. The offgas is passed through a cooling system, to make the gas
as dry as possible before analysing with a coupled gas chromatograph-mass spectrometer.
The following measurements are available:
Measurement
Broth Volume VL
Biomass concentration in broth; Cx
Biomass concentration in outflow; Cx,out
PDO concentration in broth; Cp
PDO concentration in outflow; Cp,out
Glucose concentration in broth; Cs
Glucose concentration in outflow; Cs,out
Titrant flow (2 M KOH); Fph
Feed Flow; Fin
Glucose concentration in feed; Cs,in
NH4+ concentration in outflow; CNH4+,out
NH4+ concentration in inflow; CNH4+,in
Before starting on the exercises of the case study, try making a process scheme of the
fermenter with all known and unknown flow rates and include their compositions. If you
don't know what this should look like, consult the lectures about gas and broth balances. This
will give a much better overview of the process and should aid you in the following
exercises.
Pertanyaan
1. Calculate Fout, assuming density is constant; give the correct units. Indicate which
balance you used to calculate the outflow.
Jumlah = Fin – Fout + Fph, Fout

= Fin + Fph
= 66+9 = 75 L/h
2. Which balance did you use?

A. Substrat
B. Volume
C. NH4+
The Answer : B
3. Which element conservation can you apply to check the values of RNH4+ and Rx ?
A. C
B. N
C. Charge Consevation
The Answer :B
PDO case Q2 : O2 dan CO2 trf (Tn,o &Tn,c)

Part II: O2 and CO2 transfer (Tn,o & Tn,c)

N-source. The chemostat feed, Fin in m3/h, contains glucose and (NH4)2SO4 dissolved in
water. Air sparging, with rate FN,in in mol/h, is used to supply O2 to the organism and to
remove CO2. The inflow of the dry sparged air contains much more CO2 than normal and its
composition is 21% O2; 0.5% CO2 and 78.5% N2.
Measurement Value Unit

Broth Volume VL 2.250 m3
Biomass concentration in broth; Cx 1000.0 mol/m3
Biomass concentration in outflow; Cx,out 1250 mol/m3
PDO concentration in broth; Cp 858 mol/m3
PDO concentration in outflow; Cp,out 858 mol /m3
Glucose concentration in broth; Cs 0.117 mol/m3
Glucose concentration in outflow; Cs,out 0.117 mol/m3
Titrant flow (2 M KOH); Fph 9.0 L/h
Feed Flow; Fin 66 L/h
Glucose concentration in feed; Cs,in 1292.18 mol/m3
NH4+ concentration in outflow; CNH4+,out 200 mol/m3
NH4+ concentration in inflow; CNH4+,in 500 mol/m3
Dry air inflow; FN,in 2400 mol/h
Mol fraction O2 in off gas; yo,out 0.1394 -
Mol fraction CO2 in off gas; yc,out 0.09745 -
Dissolved O2 in glucose feed; Co,in 0.30 mol/m3
Dissolved O2 in broth outflow; Co,out 0.20 mol/m3
Dissolved CO2 in broth outflow; Cc,out 3.50 mol/m3
Pertanyaan :
1. First FN,out must be calculated, which balance do you use for this?
A. Broth O2 balance
B. Gas N2 balance
C. Gas O2 balance
The Answer : B
2. Calculate FN,out (assuming the solubility of N2 in the broth is negligible); give the
correct units.
The Answer :
PDO case Q3: Ro and Rc calculation
Part III: Ro & Rc calculation

N-source. The chemostat feed, Fin in m3/h, contains glucose and (NH4)2SO4 dissolved in
water. Air sparging, with rate FN,in in mol/h3, is used to supply O2 to the organism and to
remove CO2. The dry sparged air contains much more CO2 than normal and its composition
is 21% O2; 0.5% CO2and 78.5% N2.

Pertanyaan :
1. Which balance do you use to calculate RO ?

A. Broth O2 balance
B. Gas CO2 balance
C. Gad O2 balance
D. Broth CO2 balance
The Answer : A
2. Kalkulasikan Ro
𝑑(𝑐𝑂𝑉𝐿)𝑑𝑡=0
0=𝑅𝑂+𝑇𝑁,𝑂+𝐹𝑖𝑛𝑐𝑂,𝑖𝑛+𝐹𝑜𝑢𝑡𝑐𝑂,𝑜𝑢𝑡
𝑅𝑂 = −160−0.066∗0.3+0.075∗0.20
=−160𝑚𝑜𝑙/ℎ
PDO case Q4: Data check
Part IV: Data Check
For a 1,3-propanediol (C3H8O2) – PDO – production organism (C1H1.8O0.5N0.2) a first

N-source. The chemostat feed, Fin in m3/h, contains glucose and (NH4)2SO4dissolved in
is 21% O2; 0.5% CO2 and 78.5% N2.

PDO case Q5: q-rates
Part V: q-rates

is 21% O2; 0.5% CO2 and 78.5% N2.

PDO case Q6: Charged compounds
Part VI: Charged Compounds

is 21% O2; 0.5% CO2 and 78.5% N2.

Pertanyaan :
1. A measurement of SO4 2- is performed on the broth outflow and is found to be 220

mol SO4 2- /m3 .
Which balance can you use to check this value?
A. Broth SO42- balance

B. gas SO42- balance
C. K+ balance
D. S- balance
The Answer : A
2. Which K+ concentration in mol/m3 do you expect in the broth outflow?
The Answer :
K+ yang menghasilkan KOH dan mempengaruhi pH. Maka:

𝑑(𝐶𝐾+𝑉𝐿)𝑑𝑡=0=𝑅𝐾++𝐹𝑝𝐻𝑐𝐾+,𝑖𝑛−𝐹𝑜𝑢𝑡𝑐𝐾+,𝑜𝑢𝑡
𝑐𝐾+,𝑜𝑢𝑡=0.009∗20000.075=240𝑚𝑜𝑙/𝑚3
3. The ion (SO42-, NH4+, K+) concentrations in the broth outflow are subject to a
constraint which is:
A. C-conservation
B. Charge conservation
C. N-conservation
The Answer : B
4. Does the constraint hold for the found experimental values?

The Answer : Betul

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Jecyca Thrinda 24031018004

Siti Fatimatul F 24031018022

Part I: Production Rates

For a 1,3 Propanediol (C3H8O2) – PDO – production organism (C1H1.8O0.5N0.2) a first

The following measurements are available:

Jumlah = Fin – Fout + Fph, Fout

2. Which balance did you use?

PDO case Q2 : O2 dan CO2 trf (Tn,o &Tn,c)

For a 1,3 Propanediol (C3H8O2) – PDO – production organism (C1H1.8O0.5N0.2) a first

The following measurements are available:

Measurement Value Unit

PDO case Q3: Ro and Rc calculation

Part III: Ro & Rc calculation

For a 1,3 Propanediol (C3H8O2) – PDO – production organism (C1H1.8O0.5N0.2) a first

The following measurements are available:

Measurement Value Unit

1. Which balance do you use to calculate RO ?

PDO case Q4: Data check

Part IV: Data Check

For a 1,3-propanediol (C3H8O2) – PDO – production organism (C1H1.8O0.5N0.2) a first

The following measurements are available:

Measurement Value Unit

PDO case Q5: q-rates

For a 1,3 Propanediol (C3H8O2) – PDO – production organism (C1H1.8O0.5N0.2) a first

The following measurements are available:

Measurement Value Unit

Part VI: Charged Compounds

For a 1,3 Propanediol (C3H8O2) – PDO – production organism (C1H1.8O0.5N0.2) a first

The following measurements are available:

Measurement Value Unit

1. A measurement of SO4 2- is performed on the broth outflow and is found to be 220

Which balance can you use to check this value?

A. Broth SO42- balance

2. Which K+ concentration in mol/m3 do you expect in the broth outflow?

K+ yang menghasilkan KOH dan mempengaruhi pH. Maka:

4. Does the constraint hold for the found experimental values?

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