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  • Buffer Recipes: Name Description Recipe

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    This document provides recipes for various buffers used in biological research. It lists the name, description, and ingredients to make Tris buffer, DMEM buffer, Leibovitz's L-15 medium, PBS buffer, RIPA lysis buffer, SDS-PAGE running buffer, TAE buffer, TBS buffer, Western transfer buffer, and structures of common compounds used in buffers like Tris, EDTA, SDS, NP-40, sodium deoxycholate, and Triton X-100. The buffers are used for applications like making collagen matrices, cell culture media, lysis of cells, electrophoresis, and protein transfer.

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    buffers

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    1_Buffers

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    This document provides recipes for various buffers used in biological research. It lists the name, description, and ingredients to make Tris buffer, DMEM buffer, Leibovitz's L-15 medium, PBS buffer, RIPA lysis buffer, SDS-PAGE running buffer, TAE buffer, TBS buffer, Western transfer buffer, and structures of common compounds used in buffers like Tris, EDTA, SDS, NP-40, sodium deoxycholate, and Triton X-100. The buffers are used for applications like making collagen matrices, cell culture media, lysis of cells, electrophoresis, and protein transfer.

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    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
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    13 views3 pages

    Buffer Recipes: Name Description Recipe

    Uploaded by

    pisces7390
    This document provides recipes for various buffers used in biological research. It lists the name, description, and ingredients to make Tris buffer, DMEM buffer, Leibovitz's L-15 medium, PBS buffer, RIPA lysis buffer, SDS-PAGE running buffer, TAE buffer, TBS buffer, Western transfer buffer, and structures of common compounds used in buffers like Tris, EDTA, SDS, NP-40, sodium deoxycholate, and Triton X-100. The buffers are used for applications like making collagen matrices, cell culture media, lysis of cells, electrophoresis, and protein transfer.

    Copyright:

    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
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    of 3

    http://www.geocities.com/simonwaynemoore/protocols.

    htm Page 1 of 3
    Buffer Recipes
    Name Description Recipe
    Balanced
    Salt
    50mM Tris, pH 7.5
    150mM a!l
    For 1L:
    " #.05g Tris
    " $00ml %H&'
    " $.#7g a!l
    " pH to 7.5
    " (il)te to 1*
    DMEM (10X)
    " Ma%e from pow%er
    " +se% for ma,ing collagen
    matrices for e-plant c)lt)res
    For a detailed description see
    media formulations
    " $0ml of %H&'
    " .%% (M/M pow%er 01igma 2(7777"1*3
    " Mi- thoro)ghly
    " pH to 1.$ with 10M H!l 04&0 %rops3
    " Mi- )ntil completely %issol5e%
    " 6ring to 100ml with %H&'
    " 1yringe filter 7 ali8)ot
    Leibovitz's L-15 Medim
    Invitrogen #
    41300-039 (powder)
    1141-0!4 (li"uid)
    #arious vitamins$ salts and
    amino acids
    For a detailed description see
    media formulations
    For 5L !rom po"der:
    " 9inse o)t glass :ottles with water 0no soap3
    " .)tocla5e
    " ;n a 5* :ea,er, a%% <* %istille% water
    " (issol5e 5 pac,s of pow%er
    " pH to 7.# with H!l
    " 6ring to 5* with %istille% water
    " =ilter sterili>e into a)tocla5e% :ottles )n%er sterile con%itions
    #BS
    (%&osp&ate 'uffered saline)
    pH 7.<
    137mM a!l
    &.7mM ?!l
    <.3mM a&HP'<
    1.<mM ?H&P'<
    For 1L o! 1$%:
    " $0g a!l
    " &g ?!l
    " 1<.< a&HP'<
    " &.<g ?H&P'<
    " pH to 7.<
    N#-&$ l'sis b!!er
    10mM Tris, pH 7.5
    150mM a!l
    1@ P"<0
    For 5$ml:
    " 500l of 1M Tris, pH 7.5
    " <0.75ml of %H&'
    " 5ml 10@ P"<0
    " 3.75ml of &M a!l
    R(#) l'sis b!!er
    ((adio Immuno %recipitation
    )ssa* l*sis 'uffer)
    50mM Tris, pH 7.5
    or
    10mM Phosphate, pH 7.5A
    150mM a!l
    +etergents,
    1@ P"<0
    0.1@ 1(1
    0.5@ (eo-ycholate
    For 5$ml:
    " &.5ml of 1M Tris, pH 7.5
    or &.5ml of &00mM aP, pH 7.5A
    " 35.75 of %H&'
    " 3.75ml of &M a!l
    " 5ml of 10@ P"<0
    " &.5ml of 10@ so%i)m %eo-ycholate
    " 500Bl of 10@ 1(1
    " 6ring to 50ml
    - %&osp&ate is a useful 'uffer 'ecause it in&i'its
    p&osp&atases$ &owever it will precipitate in t&e presence of
    calcium or manganese.
    SDS-#)*E
    Rnnin+ B!!er (10X)
    /&en diluted to 1X,
    0m1 2ris
    13 4+4
    000m1 5l*cine
    For 1L o! 1$%:
    " 30.3g T9;1
    " 10g 1(1
    " 1<<g Clycine
    ,$$mM Sodim #-osp-ate
    For 5$ml:
    " 1.<1g 1o%i)m Phosphate
    " <0ml %H&'
    " pH to 7.<
    " 6ring to 50ml
    5$% .)E
    (2ris acetate 6+2))
    /&en diluted to 1X,
    0.04 1 2ris - )cetate
    0.001 1 6+2)
    For 5$$ml:
    " 1&1g Tris 6ase
    " &$.#ml glacial acetic aci%
    " D.3g /(T. 0or 0ml of 0.1 6+2))
    " %H&' to 500ml
    1M .ris
    For 5$ml:
    " #.0#g Tris
    " <0ml %H&'
    " pH to 7.5 with E5ml 10M H!l
    " 6ring to 50ml
    .BS
    (2ris 'uffered saline)
    50mM Tris
    137mM a!l
    &.7mM ?!l
    For 1L:
    " $00ml %H&'
    " 1#g a!l (or !7.ml of 01 8a9l)
    " 0.&g ?!l
    " 50ml of 1M Tris, pH 7.5 (see a'ove)
    " 6ring to 1* with %H&'
    /estern
    .rans!er B!!er (10X)
    /&en diluted to 1X,
    &0mM Tris
    150mM Clycine
    For 1L o! 1$%:
    " &<.&g T9;1
    " 11&.#g Clycine
    http://www.geocities.com/simonwaynemoore/protocols.htm Page & of 3
    Strctres
    .ris ."een-,$
    ED.)
    SDS
    (4odium +odec*l 4ulfate)
    N#-&$
    (Nonidet P 40$ I56%): 9)-!30 or
    Nonylphenyl-polyethylenglycol)
    Sodim Deo%'c-olate
    nF 45
    .riton 0-1$$
    http://www.geocities.com/simonwaynemoore/protocols.htm Page 3 of 3

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