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3 Enzymes
Click on the correct answer to each question.
1 Which of the following describes an enzyme?
A a catalyst with an active site which binds to the product of a
reaction
B a fbrous protein with an active site which binds to a substrate
C a globular protein with hydrophilic groups on its surface
D an insoluble biological catalyst
2 Te graph shows the energy changes during the progress of a chemical
reaction.
Progress of reaction
1
3
2
E
n
e
r
g
y
Which of the energy changes could be decreased by adding an
enzyme?
A 1, 2 and 3
B 1 and 2 only
C 1 and 3 only
D 2 and 3 only
Multiple-choice test
Cambridge University Press 2013 Multiple-choice test: Chapter 3
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3 Which tests, carried out on samples taken at intervals during the
course of the reaction below, would enable the progress of the reaction
to be followed?
amylose reducing sugars
1 Benedicts test
2 biuret test
3 emulsion test
4 iodine in potassium iodide solution test
A 1 or 2
B 1 or 4
C 2 or 3
D 3 or 4
4 Which of the following describes the efects of temperature on an
enzyme-controlled reaction?
A At low temperatures, substrate molecules only rarely collide
with an enzymes active site.
B At low temperatures, the enzyme loses its shape and activity.
C At low temperatures, the enzyme becomes denatured.
D As the temperature increases, the number of collisions between
enzyme and substrate decrease.
5 Which statement does not describe the efect on an enzymes activity
of changing the pH.
A A pH that is very diferent from the enzymes optimum pH can
denature the enzyme.
B At low pH there are fewer hydrogen ions to interact with the R
groups of the amino acids that make up the enzyme.
C Changing the pH alters the interactions of the amino acids that
make up the enzyme.
D Changing the pH alters the enzymes three-dimensional shape.
amylase
Multiple-choice test: Chapter 3 Cambridge University Press 2013
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6 Te graph shows the efect of increasing the substrate concentration
on the rate of an enzyme-catalysed reaction.
Substrate concentration
R
a
t
e

o
f

r
e
a
c
t
i
o
n
What explains why the rate of reaction levels of?
A All the enzyme active sites are saturated with substrate.
B All the substrate has been used up.
C Te concentration of the enzyme is gradually decreasing.
D Te rate at which enzyme and substrate collide is decreasing.
Cambridge University Press 2013 Multiple-choice test: Chapter 3
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7 Curve X shows the progress of an enzyme-catalysed reaction under
optimum conditions.
Curve Y shows the same reaction with one factor changed throughout
the reaction.
Time
x
y
S
u
b
s
t
r
a
t
e

c
o
n
c
e
n
t
r
a
t
i
o
n
Which factor was changed?
A A competitive inhibitor was added.
B A non-competitive inhibitor was added.
C Te reaction took place at a diferent temperature.
D Te reaction took place at a diferent pH.
8 Tese statements are about enzyme inhibitors.
1 binds reversibly to an enzyme
2 binds to a site on the enzyme diferent from the active site
3 has a structural similarity to the enzymes normal substrate
4 can be displaced from the enzyme by a high concentration of the
enzymes normal substrate
Which statements describe a competitive inhibitor?
A 1, 2, 3 and 4
B 1, 3 and 4 only
C 2 and 3 only
D 3 and 4 only
Multiple-choice test: Chapter 3 Cambridge University Press 2013
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9 Fibrous protein from dead cells is difcult to remove from contact
lenses. Some cleaning solutions contain an enzyme to digest this
protein to soluble products.
What describes the enzyme and its activity?
A An active site on a fbrous protein binds to the enzyme and is
hydrolysed.
B An active site on a soluble product binds to the enzyme and is
digested.
C An active site on a globular protein binds to a soluble product
and digests it.
D An active site on a globular protein binds to a fbrous protein
and hydrolyses it.
10 Several diferent organisms produce lactase enzymes to hydrolyse
lactose. Te diferent enzymes have diferent molecular sizes.
Which description of these diferent lactases is correct?
A Teir active sites have the same shape.
B Teir primary structure is the same.
C Tey each contain the same number of amino acids.
D Tey have exactly the same three-dimensional structure.
Cambridge University Press 2013 Multiple-choice test: Chapter 3