Professional Documents
Culture Documents
OF MICROBIAL CONTROL
Physical Methods of Microbial Control is a part of Stataization.
Sterlaization is Process In which the Kill the Microganisms from
Contaminated thing such as glass ware Petri dish, test tube, slaid Flaks,
and also the Plateform where the Practical are performed.
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D- Filtration- These method is used for sterilizing those material which
are distrait by the effect of dry and wet heat, this metod is stable from
thermable materials such as culture media cantoning harmonies.
Enzyme and organic substrate such materials are sterlised by filtration
there the filtered of Fine poroeity which help became micro-orgnism
such as bacteria, fungi and virus.
The filters used from this process are made up of plaster of peris
intend gived and they do not allow bacteria to pass out. There for the
filtrate becomes sterile.
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The filter commonly used are chamber lamp. Pasteur filter and
berkland filter filtration is performed under pressure.
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E- Sterilization by Radiation- In this process U.V. Radiations are
generally used for stylizing culture rooms and tables in this method the
materials to be sterilized is taken in to gass tapes which to not observe
U.V. Radiations and the exposer time is about one hours.
Asmotie Pressure- Microorganism in high concentrations of salts and
sugars undergo plasmolysis molds and yeasts are more capable than
bacteria of growing in materials with law moisture of high osmotic
pressure.
Cold -Freez - It is done by liquid nitrogen dieres the biological material
a netural temp to 196C0 Deping in 20 to 18C0 in Refrigerator if we put.
The materid on at 4C0 them is is not freez the sample but the slow the
growth of the contoment.
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Distribution and Origin:- The both type of the virus papaya Ringspot P
and W is Present in Middle east, Africa, south and Central America, If is
also found in china, France, Germany, Italy, Mexico, Taiwan, USA and
also in India.
In Asia little India about 2250 years Ago. From there it is transmitted
slowly to the continent Teaching China. It is also transmitted Australia
and America Directly by India. The Papaya Introduce in India only 500
year Age so the Virus is made from the cucurbits. The Virus is switched
back and forth between Pathtypes some tiem in its evolution.
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Symptoms:- The infected papaya free show symptoms in 2-3 week. The
symptoms consists of leaf muffling and distortion, small share strong.
New leaf, dark green and slightly sunken ring on the surface of
Misshapen fruits and streaks and stem and petiols Quality on yeialed of
Infected plants are markedly Reduced.
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Properties of Virus- The papaya Ring spot Virus Consist two type of
Virus one is PRSV-P which is caused papaya Ringspot and also attack on
Cucurbits Family and another one is PRSV-W who is Infected only
Cucurbits PRSV-W also called is watermelon music virus This are single
stranded RNA and coat Proteins submit of 36 KDA
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Some species also contain plasmids or other extra chromosomal DNA.
The Chromosome very widely in between different organisms.
Compaction of the Duplicated chromosome during mitosis and meioses
result as a Four- arm Structure. It the centromere is located in the
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DNA PACKAGING:Prokaryotes do not have own nuctei, instead their DNA is organized in
to a structured celled nucleoid, The nucleoid is a structure and a
defined region of the bacterial cell, This structur is dynamic and is
maintained and remodeled by the action of a range of the Histone
protein which link with bacterial chromosomes in reechoes the DNA in
chromosome is more organized with he DNA packaged with structure
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