Tupton Hall School

Q1.(a)

Name the monosaccharides of which the following disaccharides are composed.

(i)

Sucrose
monosaccharides.....................................and.......................................
(1)

(ii)

Lactose
monosaccharides.....................................and.......................................
(1)

(b)

Amylase and maltase are involved in the digestion of starch in the small intestine.
Complete the table by identifying where these enzymes are produced and the
product of the reaction they catalyse.

Name of enzyme

Where the enzyme is

produced

Product of the
reaction catalysed
by the enzyme

Amylase
Maltase

(2)
(Total 4 marks)

Q2.

(a) A student investigated the effect of pH on the activity of the enzyme amylase.
She set up the apparatus shown in the diagram.

The tubes were made from Visking tubing. Visking tubing is partially permeable.
She added an equal volume of amylase solution and starch to each tube.

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She added a buffer solution at pH2 to tube A.

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She added an equal volume of buffer solution at pH8 to tube B.

After 30 minutes, she measured the height of the solutions in both tubes.
She then tested the solutions in tubes A and B for the presence of reducing sugars.
Describe how the student would show that reducing sugars were present in a
solution.
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...
(3)

(b)

After 30 minutes, the solution in tube B was higher than the solution in tube A.
(i)

Explain why the solution in tube B was higher.

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(3)

(ii)

The student concluded from her investigation that the optimum pH of amylase
was pH8. Is this conclusion valid? Explain your answer
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(1)(Total 7 marks)

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Q3.

(a)

Sucrose, maltose and lactose are disaccharides.

(i)

Sucrase is an enzyme. It hydrolyses sucrose during digestion. Name the

products of this reaction.
................................................... and ..................................................
(2)

(ii)

Sucrase does not hydrolyse lactose. Use your knowledge of the way in which
enzymes work to explain why.
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(2)

(b)

A woman was given a solution of sucrose to drink. Her blood glucose concentration
was measured over the next 90 minutes. The results are shown on the graph.

(i)

Describe how the womans blood glucose concentration changed in the period
shown in the graph.
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(2)

(ii)

Explain the results shown on the graph.

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(2)

(iii)

This woman was lactose intolerant.On the graph, sketch a curve to show what
would happen to her blood glucose concentration if she had been given a
solution of lactose to drink instead of a sucrose solution.
(1)
(Total 9 marks)

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M1.(a)

(i)

Glucose and fructose;

Ignore reference to alpha and beta
Either way around
1

(ii)

Glucose and galactose;

Ignore reference to alpha and beta
Either way around
1

(b)

1.

(Amylase) pancreas, produces maltose;

Place and product = 1 mark
(mark horizontally)

2.

(Maltase) in / on epithelium (of small intestine), produces glucose;

Ignore references to salivary glands or saliva
Accept wall / lining of small intestine
Ignore reference to cells alone
Ignore reference to ribosomes / rER
2

[4]

M2.

(a)

2.

1.

Add Benedicts;
Hydrolyse with acid negates mp1

Heat;
2. Accept warm, but not an unqualified reference to water
bath

3.

Red / orange / yellow / green (shows reducing sugar present);

3. Accept brown
3

(b)

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(i)

1.

Starch hydrolysed / broken down / glucose / maltose produced;

1. Neutral: Sugar produced

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2.

Lower water potential;

3.

Water enters by osmosis;

3

(ii)

Only 2 pHs studied / more pHs need to be tested;

Accept: different amylase may have a different optimum pH
1

[7]

M3.

(a)

(i)

Glucose;

Fructose;
Any order.
2

(ii)

Lactose has a different shape/structure;

Does not fit/bind to active site of enzyme/sucrase;
Only allow a second mark if reference is made to the active
site.
Max 1 mark if active site is described as being on the
substrate.
OR
Active site of enzyme/sucrase has a specific shape/structure;
Does not fit/bind to lactose;
Do not accept same shape.
2

(b)

(i)

Rose and fell;

Peak at 45 (minutes) / concentration of 6.6 (mmol dm );
3

(ii)

Glucose (produced by digestion) is absorbed / enters blood;

Decrease as used up/stored;
2

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(iii)

Curve roughly parallel to the x-axis or falling, starting

from approximately the same point;
1

[9]

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