REACTION KINETICS AND VALIDITY

OF BOD TEST FOR DOMESTIC

WASTEWATER RELEASED IN MARINE
ECOSYSTEMS
REAKSI KINETIK DAN VALIDITAS UJI
BOD UNTUK PELEPASAN LIMBAH DOMESTIK
DI EKOSISTEM LAUT

Hikmat Megandana
Master Programme of Environmental Engineering,
Institut Teknologi Bandung
Jl Ganesha No. 10, Bandung, 40132

E-mail: hikmatmegandana@yahoo.co.id

INTRODUCTION

Studies were conducted to evaluate the impact of

salinity and temperature on biodegradation of
synthetic organic compound and domestic
wastewater commonly discharged into the
marine water. This paper presents the findings of
BOD exertion of glucoseglutamic acid under
specific salinities expressed in terms of chloride
concentrations at 20C and 27C.

PURPOSE

Start

Collecting data of BOD in various concentration of chloride

and temperature

Calculating BOD which decrease by the time in

various chloride and temperature

Calculating bioremediation reaction kinetics of

BOD (k) to the reaction time in various
concentration of chloride and temperature
Analysis and Conclusion

METHODOLOGY

The natural seawater with 3536 parts per thousand (ppt) of salinity has
about 20,000 mg/L of chlorides. During present study, the chloride
concentrations were varied to represent creek, estuaries, and ocean
conditions.
The experiments were conducted for a 10-day period to achieve
maximum exertion of carbonaceous BOD.
To study the BOD exertion pattern under various salinities, the glucose
glutamic acid solution was selected as test samples.
In view of the fact that BOD exerted at 20C for 5 days is equivalent to
the BOD at 27C for 3 days (CPCB 1991) Central Pollution Control Board,,
temperatures of 20C and 270 C were considered for incubation. The
incubated samples at various time slots were analyzed for the existence
and adequacy of heterotrophic microorganisms under various chloride
concentrations using Zobel/nutrient agar media

MATERIAL

A first-order BOD decay equation has been widely

applied to describe the degradation rate of carbonaceous organic matter for most of the municipal waste.
The BOD curve can be described by a first-order
kinetics equation (Metcalf dan Eddy, Inc. 2003).
dLo/dt = - kLo

y = Lo (1-10-kt/2.303)

Dimana :
y Amount of oxygen consumed (or BOD) at time t
t Time elapsed since the start of the assay
L0 Total amount of oxygen consumed in the reaction
k Reaction constant

FORMULA

1. Percent exertion of BOD for glucose

glutamic acid solution at various chloride
concentrations at 20C

RESULT AND DISCUSSION

2. Percent exertion of BOD for glucose

glutamic acid solution at various chloride
concentrations at 27C
27o C

BOD exertion was 10% to 15% higher at 27C as compared

to 20C.
BOD decreased with increase in chloride concentration
thereby indicating the negative impact of chlorides on the
oxidation of carbonaceous matter.
Huge increase in oxidation of glucoseglutamic acid and
domestic wastewater at 27C was observed as compared to
20C emphasizing the important role of temperature.
Sudden rise in BOD exertion was not observed after the
sixth or seventh day. This indicates that adequate number
of organisms were not present in the system.
Adequate microorganisms were present at higher chloride
concentrations for the whole test period at both the
temperatures.

3. Values of k in exertion of BOD for

glucoseglutamic acid solution at various
chloride concentrations at 20C

4. Values of k in exertion of BOD for

glucoseglutamic acid solution at various
chloride concentrations at 27C

Higher values for k were observed at 27C

as compared to 20C indicating faster rate of
oxidation at elevated temperature.

The k values had lowered with increase in

chloride concentrations.

This finding confirmed the slowing down of

the rate of biodegradation of organic matter
with elevation of chlorides (salinity). more
time will be required to break down the
pollutants

Based
on the result, we can see that Ten
percent to 15% higher BOD was observed at 27C
when compared to 20C for glucose-glutamic acid.
Negative impact of chlorides on the oxidation of
organic matter was observed showing decrease in
BOD exertion at higher chloride concentrations.
The values of k reduced significantly at
elevated salinities. Good strength of the association
between degradation rate and chloride concentration.
The values of k indicate decreasing trend in
degradation with increasing salinities. The findings of
the study indicate the necessity for critical
consideration of slow degradation rates while planning
the disposal options for organic wastes in the marine
environment

CONCLUSION

ATTENTION

QUESTION

THANK YOU

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