Professional Documents
Culture Documents
Jessilyn S. Daliri
Pamela Camille A. Dagman
Hannah Faye D. Libatique
Ronalyn Pastor
July Anne Simon
BS PHARMACY 4A
Haemostasis is the process that retains the blood within the vascular
system during periods of injury. The coagulation mechanism may be thought
of as a complex series of cascading reactions involving development of
enzymes from their precursor (zymogens, procoagulants proenzymes). Most
of the substances necessary for coagulation are present in an inert form and
must be converted to an activated state. As one enzyme is formed it then
becomes available to convert the next zymogen to its activated enzyme
(serine protease). This process continues until a fibrin meshwork clot has
formed. In addition to the zymogens, protein cofactors and membrane
III. OBJECTIVES
The main objective of this study is to analyse and evaluate the
anticoagulant properties of Allium cepa. Specifically, this study aims to
accomplish the following:
1. To determine whether or not the plant extract of Allium cepa induce an
effect on the normal blood clotting time on human.
2. To formulate a product that contains Allium cepa as an active ingredients
3. To make use of Allium cepa as an alternative /supplementary
anticoagulant agent to improve and/or prevent cardiovascular diseases.
4. To recommend Allium cepa as an anticoagulant to medical practitioners
and researchers for the improvement of this study, so that it may
contribute to pharmaceutical applications and various health services.
Plant Extract
Freshly procured red onion (Allium cepa) bulbs were cut into small
slices, crushed, and dried in an oven at 70oC. Fifty (50) grams of the dried
samples were pulverized, and suspended in 100 ml of boiling water (100 oC)
for 30 min. The extract was centrifuged at 12,000 x g for 30 min, and the
supernatant was used for this experiment. (Kim et. al., 2014)
0.5 mL (eq. to 0.25 g) was introduced to the first test tube, 1 mL (eq.
to 0.5 g) into the second, and 2 mL (eq. to 1 g) into the third test tube.
Sample Collection and Analysis
The participants had been chosen according to the following criteria:
having
normal
PT,
not
suffering
from
any
cardiovascular
diseases
Verbal consent was obtained from ten (10) human volunteers. Blood
samples were extracted with the help of medical technologists. Blood
samples of shortlisted donors
were subjected to basic hematology screening tests to exclude those with
abnormal results.
concentration
were
analyzed
using
standard
methods.
(Cheesbrough, 2000)
b. List of materials needed
Mortar and pestle
Whatman No. 1 filter
paper
Funnel
Erlenmeyer flask
Beaker (50 mL, 100
mL, 250 mL)
Dropper
c. List of reagents (include quantity)
Red onion (Allium cepa L.) bulbs
Distilled water
EDTA/Trisodium citrate
d. List of machines/ equipment needed
Analytical balance
Centrifuge
Sucking pump
Oven
person/organization
in
Domingo
A.
Madulid,
Division
Chief,
527-1218
botany@nationalmuseum.gov.ph)
The blood samples obtained from the 10 participants will be screened
with the help of 4J Clinical Diagnostic Laboratory.