Professional Documents
Culture Documents
ECOLOGY OF
FRESHWATER FISH
Letter
Ciesielski S, Makowiecki D. Ancient and modern mitochondrial haplotypes of common bream (Abramis brama L.) in Poland.
Ecology of Freshwater Fish 2005: 14: 278282. Blackwell
Munksgaard, 2005
Abstract Genetic data on archaeological specimens provide
complementary information for addressing questions on distribution and
migration of shes over long time scales. In this study DNA was extracted
from common bream bones (N 4), dated c. 60001000 bp, and a 172 bp
fragment of mitochondrial cytochrome b gene has been sequenced. The
obtained sequences differed from homologous sequences of breams living
contemporarily (N 4), inhabiting the same geographical areas as
ancient sh. None of ancient mitochondrial haplotype was found in sh
living at present. Our results suggest that sh vicariance could be affected
also by other than glacial retreat historical events, and the mechanisms that
inuenced present distribution of freshwater shes are still unclear.
1
Department of Environmental Biotechnology,
Faculty of Environmental Sciences and Fisheries,
University of Warmia and Mazury in Olsztyn,
Olsztyn, 2Institute of Archaeology and Ethnology,
Polish Academy of Sciences, Poznan, Poland
Collection location
Sample
Age (years)
Dabki 20
Dabki 01
Dudka 25
Dudka 01
Wolin 11
Wolin 12
Wolin 01a
Wolin 01b
os cleithrum
Muscle
os operculum
Muscle
os cleithrum
os cleithrum
Muscle
Muscle
6000
1
5500
1
1000
1000
1
1
Primers L: 5-acgcactagtcgacctcc-3 and H: 5-acgtctcggcagatgtgg-3 spanned a 172 bp region of cytochrome b gene from 46 to 218 nucleotide (Ciesielski
et al. 2002).
Double-stranded PCR amplication was performed
in 50 ll reaction volumes containing 2 units of Taq
DNA polymerase (Promega), 5 ll reaction buffer
(500 mm KCl, pH 8.5; Triton X-100), 20 pmol of
each primer (MWG-BIOTECH, Ebersberg, Germany),
2.5 mm MgCl2, 500 of lm dATP, dCTP, dGTP, and
dTTP, and 2 ll of DNA template. DNA was amplied
using a Perkin Elmer 9600 thermal cycler (PE-Applied
Biosystems, Foster City, CA, USA) beginning with
preliminary denaturation at 95 C for 5 min. The
amplication cycle consisted of 94 C for 30 s, 52 C
for 30 s, and 72 C for 45 s, for a total of 30 cycles,
ending with a nal elongation step at 72 C for 3 min.
Initial PCR products of archival samples were reamplied using the same procedure.
The PCR product was separated by electrophoresis
on 1.5% agarose gel, using 1x TBE buffer (0.5 m
EDTA, pH 8.0). Ethidium bromide (0.1 mg/ml) was
added to the gel and the PCR product was visualised
with UV light. Molecular weight marker UX 174/Hinf
I was used.
279
51
61
63
67
69
72
Dudka 01
Dabki 01
Wolin 01a
Wolin 01b
Dudka 25
Dabki 20
Wolin 11
Wolin 12
A
A
A
C
C
1.
2.
3.
4.
5.
Con 01
Dab 20
Wol 12
Dud 25
Wol 11
0.03516
0.03502
0.00862
0.00860
0.03516
0.02615
0.02632
0.02609
0.02615
0.01730
281
Acknowledgements
We thank M. Luczynski and F. Volckaert for critical reading
of the manuscript. We are grateful also to J. Filipiak,
Z. Chelkowski, K. Formicki, and A. Wisniewska for their kind
provision of samples. This research was supported by the Polish
Foundation for Sciences under the ARCHEO Nr 5 scheme.
References
Adachi, J. & Hasegawa, M. 1996. Model of amino acid
substitution in proteins encoded by mitochondrial DNA.
Journal of Molecular Evolution 42: 459468.
Avise, J.C. 1994. Molecular markers, natural history and
evolution. London: Chapman and Hall.
Bernatchez, L. & Osinov, A. 1995. Genetic diversity of trout
(genus Salmo) from its most eastern range based on
mitochondrial DNA and nuclear gene variation. Molecular
Ecology 7: 431452.
Briolay, J., Galtier, N., Brito, R.M. & Bouvet, Y. 1998.
Molecular phylogeny of Cyprinidae inferred from cytochrome b DNA sequences. Molecular Phylogenetics and
Evolution 9: 100108.
Ciesielski, S., Brzuzan, P. & Luczynski, M. 2002. Recovery and
analysis of mitochondrial DNA from ancient bones of
common bream, Abramis brama L. Ancient Biomolecules
4: 4346.
Cooper, A. & Wayne, R. 1998. New uses for old DNA. Current
Opinion in Biotechnology 9: 4953
Durand, J.D., Persat, H. & Bouvet, Y. 1999. Phylogeography
and postglacial dispersion of the chub (Leuciscus cephalus)
in Europe. Molecular Ecology 8: 989997.
Hagelberg, E., Bell, L.S., Allen, T., Boyde, A., Jones, S.J. &
Clegg, J.B. 1991. Ancient bone DNA: techniques and
applications. Philosophical Transactions of the Royal Society,
London B 333: 399407.
Hewitt, G.M. 1996. Some genetic consequences of ages, and
their role in divergence and speciation. Biological Journal of
Linnean Society 58: 247276.
Hewitt, G.M. 1999. Post-glacial re-colonization of European
biota. Biological Journal of Linnean Society 68: 87112.
Hewitt, GM. 2004. The structure of biodiversity insights from
molecular phylogeography. Frontiers in Zoology 1: 4.
282