HDLMANUAL

PFF

50 uL CHEMA PRECIPITATE REAGENT

50 Ul Px SAMPLE
CENTRIFUGE FOR 10 MINS
1.) REAGENT 1 1mL OF CHEMA TOTAL
CHOLE REAGENT.
2.) 25 ul FROM PFF
3.) INCUBATE
FOR 5 MINS.
4.) READ IN ABS (500 nm)
5.) STANDARD : 0.664
CALCULATION
U
200
MG/DL
S
GLUCOSE
(PROGRAM
MED)
READ IN Hb
1.) GLUCOSE REAGENT 1,000 ul.
2.) PLUS SAMPLE OF Px OF 10 ul.
3.) INCUBATE FOR 5 MINS.
4.) READ AS IS.
CREATIN
INE
(PROGRA
MMED)
1.) READ IN CREA
2.) 250/500 ul OF REAGENT A (BASE)
3.) 250/500 ul OF REAGENT B (PICRIC)
4.) 25/50 ul OF Px SAMPLE
5.) NO INCUBATION (KINETIC)

UREA
(PROGR
AMMED)
1.) READ IN UREA
2.) 1000 ul OF REAGENT
3.) READ THE REAGENT BLANK (W/C WILL BE 500ul)
4.) ADD 5 ul OF SAMPLE
5.) READ..NO INCUBATION (KINETIC)

TRIGLYCE
RIDE
(ABSORBA
NCE)
READ IN TG
1.) 500 ul OF TG REAGENT
2.) ADD 5 ul OF Px SAMPLE
3.) INCUBATE FOR 5 MINS
4.) 505 nm
U
2.26
S
STD : 0.458
URIC
ACID
(ABSORB
ANCE)
1.) 500 ul OF REAGENT
2.) 15 ul OF Px SAMPLE
3.) INCUBATE FOR 5 MINS
4.) 505 nm
U
0.30
S
STD : 0.238

GLUCOSE
(ABSORBA
NCE)
1.)500 ul OF REAGENT
2.) 5 ul OF Px SAMPLE
3.) INCUBATE FOR 5 MINS
4.) 505 nm
U
5.56
S
STD : 0.183