Bahir Dar University

College of Agriculture and Environmental Sciences

Animal Production and Technology Program
Course Title
Biotechnology in Animal Breeding
Course Code
Angb636
M.Sc. Program
Animal Genetics and Breeding
Mode of delivery parallel
Module No.
02
Instructor
Office location: 30
Mobile:0931855328 ; e-mail: garikikumar@yahoo.com
ECTS Credits
5
(CP)
Contact Hours
Lecture
Tutorial
Lab/Practical Home Study
(per week)
2
8
Lecture days,
Friday 8.30-10.15am
Hours & Room:
Tutorial/Lab
NA
days & Hours
Target Group:
M.Sc. in Animal Genetics and Breeding
Year /Semester
Year 1 & semester 2
Pre-requisites
Status of the
Compulsory
course

Total
5

COURSE DESCRIPTION
Introduction: The basic tools for genome analysis. Molecular technology. Methods of gene
isolation. Genetic engineering: insertion of foreign DNA and, gene transfer. Evaluation and
dissemination of transgenic in animal production. Biochemical and DNA polymorphism.
QTLs (Quantitative Trait Loci), Marker genes and gene mapping. Marker assisted selection in

animal breeding. PCR; Embryo manipulation. Embryonic stem cells and its application in
animal breeding.
Course objectives: At the end of this course, the student will be able to:
In depth understanding of Biotechnology and genomics.
Increased awareness of the importance of genome analysis in research and
applications.
Expected/learning outcomes: students should able to know the different biotechnology
procedures in the Animal genetics and breeding

week

Lecture
(hrs)

Conceptual Focus

Introduction basic tools -Listen to a lecture

for genome analysis
and take notes on

Methods of gene isolation.

Eukaryotic organelles and
accessing the genome

4,5

Methods of inserting of foreign

DNA,
Gene
transfer
technology.
Transformation , transfection
and somatic cell cloning
Primary and secondary cell
culture
Continuous cell lines.
Application of cell culture
technology in animal viral
vaccines and pharmaceutical
proteins

6
2

Activities/tasks

Structure of sperm and ovum

Cryopreservation of sperm and
ova
Artificial insemination
Superovulation

-Synchronization of estrous
Advantages and disadvantages

the lesson treated,

-Take part in
reading
assignment,
-Assignment
submission
-Forward all the
confusion or
doubts students
may have in
relation to the
given lecture
-Literature review
and
presentati
on
(seminar)
-Listen to a lecture
and take notes on
the lesson treated,
-Take part in
reading
assignment,
-Assignment
submission
-Forward all the

Cryopreservation of embryos
Ethics of embryo manipulation

8,9

10

confusion or
doubts students
may have in
Transgenic manipulation of -Listen to a lecture
animal embryos
and take notes on
Different
applications
of the lesson treated,
transgenic animal technology
-Take part in
Animal Viral vectors
reading
assignment,
-Forward all the
confusion or
doubts students
may have in
relation to the
given lecture
-Literature review
and
presentati
on
(seminar)
Animal cloning basics
Cloning from embryonic stem
cells and adult cells
Cloning different animals
Cloning for conservation and
conservation of endangered
species
Social and ethical issues of
cloning
Biochemical
and
DNA
polymorphism
Different
methods
in
characterization of animal
genomes

11

12

STR, QTL, RFLP, RAPD

Genetic basis of disease
resistance

13

Transgenic animal production

and expression of therapeutic
proteins

Listen to a lecture
and take notes on
the lesson treated,
-Take part in
reading
assignment,
-Assignment
submission
-Forward all the
confusion or
doubts students
may have in
relation to the
given lecture

14

Immunological and nucleic

acid based detection methods
for identification of animal
species. PCR

15

Marker assisted selection in

animal breeding

16

Embryonic stem cell and

applications in animal breeding

Course teaching/learning activities

The mode of the delivery of the course combines lectures, discussion, questioning and
answering, readings, assignments, individual and /or group works and presentation.
Assessment Methods
Evaluation will be carried out based on continuous assessment which comprises:

midterm
Assignment
seminar
Final exam

Grading: As per the universitys regulation

COURSE POLICY
All students are expected to abide by the code of conduct of students
(article 166 and 166.1.1, of The Senate Legislation of Bahir Dar University
May 20, 2005) throughout this course. Academic dishonesty, including
cheating, fabrication, and plagiarism will not be tolerated and will be
reported to concerned bodies for action. Class activities will vary day to
day, ranging from lectures to discussions. Students will be active
participants in the course. You need to ask questions and raise issues. You
are expected to do all the assignments submit and present you are
supposed to accomplish. You are expected to attend class regularly. If you

miss more than 85% of the class attendance you will not sit for final
exams. Please try to be on time for class. Cell phones MUST be turned of
before entering the class as they are disruptive and annoying to all of us
in the class. You are responsible for all class announcements and changes.
REFERENCES
Reproduction in farm animals-Hafeez and Hafeez 2008
Principles of genome analysis and genomics-SP primrose and RM twyman 2008
Discovering Genomics , proteomics and Bioinformatics- A Malcome Campbell and Laurie
heyer 2010
Understanding Biotechnology- Aluizio Borem, Fabricio R santos and David Brown 2009
Genes VI Benjamin Lewin.

Approval section
Course instructor:

Dilip Garikipati

___________________________

Course chair:

Damitie Kabede

____________________________

Department Head:

Fentahun Meheret

____________________________

ViCe Dean

Hailu Mazengia

___________________________