Analyzing R2

Using NMRView to obtain R2 values

1. Obtaining R2 values using NMRView is very similar to how R1 values were
obtained.
2. Open NMRView, and open all your R2 .nv spectra (ctrl allows you to select
multiple files). Then open and draw your smallest timescale (e.g. 10msa).

3. Open your peaklist, and add it to your spectra and change the color of the
boxes to blue. Then align the centers of the boxes to their respective peaks
by using the selector cursor.

4. Once all the peaks are aligned, open up the Rate Analysis window. The
setup is similar as before; however instead of T1 in the case of R1, you want
the relaxation type to be T2.

5. After you have all the settings set, time tables loaded, and have measured all
the values and fit the table, save the table in the folder with all your R2 data.

Using LibreOffice to Graph Data

6. Now you want to open up the data you saved in LibreOffice. Again the
method of opening it up is very similar, and you will see a similar display as
you did with R1 (e.g. Peak, N, timescales, residue, a, etc.)

7. You want to copy both your b and bsigma values, and paste them in a new
sheet, and multiply them both by 1000. Your b*1000 value will be R2 and
your bsigma*1000 will be your error.

8. Just as with R1 youll want to calculate the 10% average, standard deviation,
and +/- of your average. The data will still be in terms of peak number, so
youll have to change it in terms of residue number.

9. Graphing R2 is identical to graphing R1. Youll have 3 lines, you average

and the +/-. R1 ranges from .5-3. R2 ranges from 10-40.