Discussion Questions for Isoelectric Point Lab

1. What is the molecular basis for the differences in the electrophoretic

mobilities of the four proteins analyzed in this exercise?
2. Explain how your results would be affected if the electrophoretic
separation was carried out at pH 10.5.
3. at pH 6.0?
4. At a pH of 7, acidic amino acids are __________ charged so they move
toward the __________ electrode. At a pH of 7, basic amino acids are
__________ charged to they move toward the __________ electrode.
5. Why would a protein remain stationary in an electrical field if the pH of the
electrophoresis buffer is equal to its isoelectric point?