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Determination of The Isoelectric Points of Casein
Determination of The Isoelectric Points of Casein
Points of Casein
Principle*
Using acetate buffer of different PH values the
approximate position of the isoelectric point of
casein can be obtained by determining the PH
of minimum solubility.
The PH of any solution can be calculated from
Handerson-hasselbalch equation.
PH = Pka + log [Sodium acetate]
[acetic acid]
Tube #
8.4
1
8.8
1
8.5
1
8
0.2
0.5
1
7
7.4
0.6
1
-
1
2
1.6
* Results
Tube #
1
2
3
4
5
6
7
ppt at 0
min
ppt at 10
min
ppt at 30
min
PH
* Results
PK acetic acid = 4.5
PH = PKa + log
[CH3COONa]
[CH3COOH]
Samar A. Damiati