Professional Documents
Culture Documents
Phosphate Buffer (PB)
Phosphate Buffer (PB)
0.1 M
NaH2PO4 * H2O
Na2HPO4 (anhydrous)
3.1 g
10.9 g
Distilled water to make a total volume of 1 liter. The pH of the final solution will be 7.4.
This buffer can be stored for up to 1 month in 4C.
0.2 M
NaH2PO4 * 2 H2O
Na2HPO4 (anhydrous)
5.93 g
23.0 g