Test method

Developed for

Process

BioGents Cage Test

Impregnated textiles,
crmes, lotions, liquid
formulations

Volunteer exposes arm via

window to mosquitoes

EPA Petri-Dish Bioassay

to establish baseline
susceptibility data under
lab/greenhouse conditions

To investigate the toxicity of

other impregnated surfaces than
bed nets

Time

Test every 30min, up to

8hours after application

Number of
Mosquitoes

30 Mosquitoes in a cage

Treated fabric is placed

in the inverted lid of a
Petri-dish
10-15 female mosquitoes
are exposed for 2 min
and then removed
further observation

Mosquito knock-down is
documented after 15 and
60 min

Each treated fabric is tested

on at least 100 mosquitoes.

Criteria/
Problems

Recommendation
Price per
sample

Pictures of
Test method

65,- + VAT

EPA specifies 2 min for

the exposure but this
might be too short for
impregnated apparel
Knock-down in %

150,- + VAT; control sample

not included

WHO Tube Test

To verify the susceptibility of
wild mosquito populations to
certain insecticides

standard WHO plastic cone

is attached to the test
surface
5 female mosquitoes are
exposed for 3min and then
removed
further observation

no. of immobilized, knockeddown mosquitoes are

documented after 1h
mortality is checked after
24h

Each textile is tested in 10

repetitions = 50 test mosquitoes

Complete protection time

until 1st bite
Protection time until 2nd
bite (or 1st confirmed
bite)
Time until break-off
(number of bites on
treated arm exceeds 5%
of the bites on the control
arm protection less
than 95%)
5 volunteers and 3
mosquitoes species

WHO Cone Test

WHO recommends 3 min for

the evaluation of treated bed
nets but this might be too
short for impregnated
apparel
WHO gives no recommenddations for apparel
Inner surface of cone not
completely covered by
textile
Knock-down in %

textile is attached to the

inner walls of the tube
10 female mosquitoes are
exposed for 3 min and
then removed
further observation

no. of immobilized,
knocked-down mosquitoes
are documented after 1h
mortality is checked after
24h

Each textile is tested in 5

repetitions = 50 test
mosquitoes

Test according to
TL 8305-0331
Impregnated battle dress
uniforms and is widely
used by the Military
100 times washed
sample is attached to
the inner walls of
WHO tubes
10 female insects are
introduced
observation of the
mosquitoes
Times of knock-down
of the 1st, 5th and 10th
insects are
documented.
71,512,0 min is
maximum for yellow
fever mosquitoes
Each textile is tested in 10
repetitions = 100 test
mosquitoes

WHO recommends 3min

BioGents defines the
exposition time after
consultation with the
customer
Knock-down in %

All mosquito species possible

All mosquito species possible

125,- + VAT; control sample

not included

125,- + VAT; control sample

not included

480,- + VAT; above 5

samples 10% discount