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  • Region Orient. 5'position Sequence: Page 1 / 2

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    The document describes primer sequences used for amplifying different genomic regions of human rhinovirus C (HRV-C), including the 5'UTR, VP4, VP1, and 3Dpol regions. It provides the orientation, 5' position, and sequence for each primer. A figure is referenced that shows a comparison of HRV-C phylogenies based on the 5' and 3' ends of the 3Dpol fragment.

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    Tester 021

    Original Title

    Supplementary Data

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    The document describes primer sequences used for amplifying different genomic regions of human rhinovirus C (HRV-C), including the 5'UTR, VP4, VP1, and 3Dpol regions. It provides the orientation, 5' position, and sequence for each primer. A figure is referenced that shows a comparison of HRV-C phylogenies based on the 5' and 3' ends of the 3Dpol fragment.

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    9 views2 pages

    Region Orient. 5'position Sequence: Page 1 / 2

    Uploaded by

    Academe Home Tuition
    The document describes primer sequences used for amplifying different genomic regions of human rhinovirus C (HRV-C), including the 5'UTR, VP4, VP1, and 3Dpol regions. It provides the orientation, 5' position, and sequence for each primer. A figure is referenced that shows a comparison of HRV-C phylogenies based on the 5' and 3' ends of the 3Dpol fragment.

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    © All Rights Reserved
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    of 2

    TABLE S1

    PRIMER SEQUENCES USED FOR AMPLIFICATION


    OF 5UTR, VP4, VP1 AND 3Dpol REGIONS
    Region

    Orient.a

    3DPol
    OS
    3DPol
    OAS
    3DPol
    IS
    3DPol
    IAS
    UTR-C(1) S
    UTR-A(1) S
    UTR(1) OAS
    UTR-A(1) OAS
    UTR-C(1) OAS
    UTR-A(1) IAS
    UTR-C(1) IAS
    UTR(2) S
    UTR(2) OAS
    UTR(2) IAS
    UTR(3) S
    UTR(3) OAS
    UTR(3) IAS
    VP1
    OS
    VP1
    OAS
    VP1(1)
    IS
    VP1(1)
    IASa
    VP1(1)
    IASb
    VP1(2)
    IS
    VP1(2)
    IAS
    VP4/VP2 OS
    VP4/VP2 OAS
    VP4/VP2 IS
    VP4/VP2 IAS

    5Positionb

    Sequence

    6300
    6901
    6360
    6877
    1
    1
    375
    375
    375
    355
    354
    164
    560
    550
    445
    1066
    868
    1986
    3449
    2256
    2852
    2852
    2745
    3151
    445
    1104
    533
    1066

    TGT GAT CCW GAY RTY TTY TGG TCA C


    ACR TGR TCT TSW GTR TTC TTT GG
    TTT GAY TAY ACM AAY TAT GAT GG
    TCY YTD GTC CAT CTR ATT GAY TCA
    TTM AAA CAG CSG BNN GGT TGY ACC CA
    TTM AAA CTG RRW BYR GGT TGY TCC CA
    GCA GGS MRC CAC GCR GGC T
    GCA GGC AGC CAC GCA GGC T
    GCA GGG CAC CAC GCG GGC T
    GAH CAC TGT YRC CAG TGG GG
    GAC ACC GTC GCC GGT GGG G
    HCA AGY ACT TCT GTY WCC CCS G
    RGA AAC ACG GAC ACC CAA AGT AGT
    ACA CCC AAA GTA GTY GGT YCC R
    CCG GCC CCT GAA TGY GGC TAA
    TCW GGH ARY TTC CAM CAC CAN CC
    ATA GTR ATT TGY TTD AGC CTA TCD GAV A
    ATR GCM TAC ACM CCH CCW GG
    YTT VCC WCC ACA RTC HCC WGG
    ATG HTR CGW GAY ACW CCH ATG AT
    ATC ATA HCC ATC ATA RAA VAT GTA RTA
    ATC ATA HCC ATC ATA RAA VAC ATA RTA
    GCY TCH AAC CCH AGT GTN TTY T
    GTR TGN ACA WAH AKR TCA CTR GGT CC
    CCG GCC CCT GAA TGY GGC TAA
    ACA TRT TYT SNC CAA ANA YDC CCA T
    ACC RAC TAC TTT GGG TGT CCG TG
    TCW GGH ARY TTC CAM CAC CAN CC

    Orientation: OS: outer sense; OAS: outer antisense; IS: inner sense; IAS: inner antisense
    5 base position numbered according to the EF582385 reference sequence

    Page 1 / 2

    FIGURE S1
    COMPARISON OF HRV-C PHYLOGENIES AT THE
    5 AND 3 ENDS OF THE 3Dpol FRAGMENT

    Page 2 / 2

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